cDNA Clone PPM1F

• Synonyms: CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase
• Plasmid: pGEX6P1 PPM1F
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C PPM1F
• Genbank: NM_014634
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCTCCTCTGGAGCCCCACAGAAGAGCAGCCCAATGGCCAGTGGAGC
TGAGGAGACCCCAGGCTTCCTGGACACGCTCCTGCAAGACTTCCCAGCCC
TGCTGAACCCAGAGGACCCTCTGCCATGGAAGGCCCCAGGGACGGTGCTC
AGCCAGGAGGAGGTGGAGGGCGAGCTGGCTGAGCTGGCCATGGGCTTTCT
GGGCAGCAGGAAGGCCCCGCCACCACTTGCTGCTGCTCTGGCCCACGAAG
CAGTTTCACAGCTGCTACAGACAGACCTTTCCGAATTCAGGGAGTTGCCC
AGGGAGGAAGAAGAAGAGGAGGAGGACGATGACGAGGAGGAAAAGGCCCC
TGTGACCTTGCTGGATGCCCAAAGCCTGGCACAGAGTTTCTTTAACTGCC
TTTGGGAAGTCGCCGGCCAGTGGCAGAAGCAGGTGCCATTGGCTGCCCGG
GCCTCACAGCGGCAGTGGCTGGTCTCCATCCACGCCATCCGGAACACTCG
CCGCAAGATGGAGGACCGGCACGTGTCCCTCCCTTCCTTCAACCAGCTCT
TCGGCTTGTCTGACCCTGTGAACCGCGCCTACTTTGCTGTGTTTGATGGT
CACGGAGGCGTGGATGCTGCGAGGTACGCCGCTGTCCACGTGCACACCAA
CGCTGCCCGCCAGCCAGAGCTGCCCACAGACCCTGAGGGAGCCCTCAGAG
AAGCCTTCCGGCGCACCGACCAGATGTTTCTCAGGAAAGCCAAGCGAGAG
CGGCTGCAGAGCGGCACCACAGGTGTGTGTGCGCTCATTGCAGGAGCGAC
CCTGCACGTCGCCTGGCTCGGGGATTCCCAGGTCATTTTGGTACAGCAGG
GACAGGTGGTGAAGCTGATGGAGCCACACAGACCAGAACGGCAGGATGAG
AAGGCGCGCATTGAAGCATTGGGTGGCTTTGTGTCTCACATGGACTGCTG
GAGAGTCAACGGGACCCTGGCCGTCTCCAGAGCCATCGGGGATGTCTTCC
AGAAGCCCTACGTGTCTGGGGAGGCCGATGCAGCTTCCCGGGCGCTGACG
GGCTCCGAGGACTACCTGCTGCTTGCCTGTGATGGCTTCTTTGACGTCGT
ACCCCACCAGGAAGTTGTTGGCCTGGTCCAGAGCCACCTGACCAGGCAGC
AGGGCAGCGGGCTCCGTGTCGCCGAGGAGCTGGTGGCTGCGGCCCGGGAG
CGGGGCTCCCACGACAACATCACGGTCATGGTGGTCTTCCTCAGGGACCC
CCAAGAGCTGCTGGAGGGCGGGAACCAGGGAGAAGGGGACCCCCAGGCAG
AAGGGAGGAGGCAGGACTTGCCCTCCAGCCTTCCAGAACCTGAGACCCAG
GCTCCACCAAGAAGCTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSSGAPQKSSPMASGAEETP
GFLDTLLQDFPALLNPEDPLPWKAPGTVLSQEEVEGELAELAMGFLGSRK
APPPLAAALAHEAVSQLLQTDLSEFRELPREEEEEEEDDDEEEKAPVTLL
DAQSLAQSFFNCLWEVAGQWQKQVPLAARASQRQWLVSIHAIRNTRRKME
DRHVSLPSFNQLFGLSDPVNRAYFAVFDGHGGVDAARYAAVHVHTNAARQ
PELPTDPEGALREAFRRTDQMFLRKAKRERLQSGTTGVCALIAGATLHVA
WLGDSQVILVQQGQVVKLMEPHRPERQDEKARIEALGGFVSHMDCWRVNG
TLAVSRAIGDVFQKPYVSGEADAASRALTGSEDYLLLACDGFFDVVPHQE
VVGLVQSHLTRQQGSGLRVAEELVAAARERGSHDNITVMVVFLRDPQELL
EGGNQGEGDPQAEGRRQDLPSSLPEPETQAPPRS*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 PPM1F75.37 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU3020
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression. As genbank except for R132C change.
• Low Temp: No