cDNA Clone ROCK2 (rat)

• Synonyms: ROCK2
• Plasmid: pFBHTb ROCK2 D544-1380
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-ROCK2 D544-1380
• Genbank: NM_013022
• Tag: 6His
• Species: Rat

Sequence of Insert

GGATCCCCCGGCGCCCCCGAGGCCGCGCCGAGGGACGGGGCGGGCGCGGG
TCGTCAGAGGAAGCTGGAGGCGCTCATCCGAGACCCTCGCTCGCCCATCA
ACGTGGAAAGCCTGCTGGATGGCTTAAATTCCTTGGTCCTTGATTTGGAT
TTTCCTGCTTTGAGGAAAAATAAAAATATAGATAATTTCCTAAATAGATA
TGAGAAAATTGTGAAAAAAATCAGAGGTTTACAGATGAAAGCAGAAGACT
ATGATGTTGTAAAAGTTATTGGAAGAGGTGCTTTTGGTGAAGTTCAGTTG
GTTCGTCATAAGGCATCACAGAAGGTTTATGCAATGAAGCTTCTTAGTAA
ATTTGAAATGATAAAAAGATCAGATTCTGCTTTTTTCTGGGAAGAAAGAG
ATATTATGGCTTTTGCTAACAGTCCGTGGGTGGTTCAGCTCTTTTGTGCC
TTTCAAGATGACAGGTATCTGTACATGGTGATGGAGTACATGCCAGGTGG
AGACCTTGTTAACCTGATGAGCAATTATGATGTGCCTGAGAAGTGGGCCA
AGTTTTATACTGCTGAAGTCGTGCTTGCACTGGATGCAATACACTCCATG
GGCTTAATACACAGAGATGTGAAGCCCGATAACATGCTATTGGATAAACA
CGGACATCTAAAATTAGCAGATTTTGGCACATGTATGAAAATGGATGAAA
CAGGCATGGTGCATTGTGATACAGCAGTTGGAACTCCAGACTACATATCA
CCTGAGGTTCTGAAATCACAAGGAGGTGATGGTTACTATGGACGAGAATG
TGATTGGTGGTCTGTAGGTGTTTTCCTTTTTGAGATGTTAGTAGGGGACA
CTCCATTTTATGCAGATTCACTTGTAGGAACCTACAGCAAAATTATGGAT
CATAAAAATTCACTGTGTTTTCCTGAAGATACAGAAATTTCTAAACATGC
GAAGAATCTCATATGTGCCTTCTTAACAGACAGGGAGGTACGACTTGGAA
GAAATGGGGTAGAAGAAATCAAATCAGCATCCTTCTTTAAGAATGATCAG
TGGAATTGGGATAACATAAGAGAGACGGCAGCTCCAGTGGTGCCTGAGCT
CAGCAGTGACATAGACAGCAGCAACTTTGATGACATTGAGGATGACAAAG
GCGATGTAGAGACCTTCCCGATTCCTAAAGCTTTTGTGGGAAATCAGCTG
CCTTTTATAGGATTTACCTACTTTAGAGAAAATTTGTTACTAAGTGATTC
TCCACCTTGTAGAGAAAATGATGCAATACAAACAAGGAAAAGTGAAGAAA
GTCAGGAGATTCAGAAAAAATTATATGCACTAGAAGAACACCTTAGCAGT
GAGGTACAAGCCAAGGAGGAACTGGAACAGAAGTGCAAGTCTATTAATAC
TCGTCTAGAGAAAACAGCAAAGGAACTAGAAGAAGAGATTACCTTTAGGA
AAAATGTGGAATCAACATTAAGACAGCTAGAAAGAGAAAAGGCCCTTCTT
CAGCACAAAAATGCAGAATATCAACGGAAAGCTGATCATGAAGCTGACAA
GAAACGGAATTTGGAAAATGATGTTAACAGCTTAAAAGATCAACTTGAAG
ATTTGAAGAAAAGAAACCAGAGCTCTCAGATATAAGGATCC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSPGAPEAAPRDGAGAGRQRKLEA
LIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKI
RGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRS
DSAFFWEERDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVNLMS
NYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLAD
FGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGV
FLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLICAF
LTDREVRLGRNGVEEIKSASFFKNDQWNWDNIRETAAPVVPELSSDIDSS
NFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYFRENLLLSDSPPCREND
AIQTRKSEESQEIQKKLYALEEHLSSEVQAKEELEQKCKSINTRLEKTAK
ELEEEITFRKNVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLEND
VNSLKDQLEDLKKRNQSSQI*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1875
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His tag for baculovirus expression