cDNA Clone SAP97 (rat)

• Plasmid: pGEX6P-3-SAP97
• Parent Plasmid: pGEX6P3
• Expressed: GST-SAP97
• Genbank: U14950
• Tag: GST
• Species: Rat

Sequence of Insert

GAATTCGCCCTTGCGGTACCCGGTCCGGAAGCAAGATACCCAGAGAGCAT
TGCATCTGTTGGAAGAATATCGGTCGAAACTAAACCAAACCGAAGACAGA
CAGCTCAGGAGTTCCATAGAGCGGGTTATTAGCATATTTCAGAGCAACCT
CTTTCAGGCTTTAATAGACATTCAAGAATTTTATGAAGTGACCTTACTTG
ATAATCCAAAATGTGTGGATCATTCAAAGCAGTGTGAACCGGTCCAACCC
GGGAATCCTTGGGAGAGTGGCAGCCTTTCAAGTGCTGCTGTGACTTCAGA
GAGCCTGCCCGGCGGCCTTAGCCCTCCAGTAGAGAAATACCGGTATCAGG
ACGAAGAGGTGCTGCCTTCAGAGCGGATTTCCCCGCAAGTCCCAAATGAG
GTGCTGGGTCCGGAGCTGGTTCACGTCTCGGAGAAGAGCCTGTCAGAGAT
TGAGAATGTCCACGGGTTTGTCTCTCACTCTCATATCTCACCCATAAAGG
CAAATCCTCCTCCTGTGCTGGTCAACACAGACAGCTTGGAGACACCAACT
TATGTTAATGGTACTGATGCAGATTATGAATATGAGGAAATCACACTTGA
AAGGGGAAATTCAGGCCTTGGTTTCAGCATTGCAGGAGGTACAGACAACC
CACACATTGGAGATGACTCAAGTATTTTCATCACCAAAATTATCACGGGA
GGAGCAGCTGCCCAGGATGGAAGATTGCGGGTAAATGACTGTATACTGAG
AGTAAATGAAGCAGATGTTCGTGATGTAACCCACAGCAAAGCAGTTGAAG
CATTGAAAGAAGCTGGATCGATAGTGCGATTATATGTGAAAAGGCGGAAG
CCAGCCTCAGAAAAAATCATGGAAATAAAACTTATTAAAGGCCCTAAAGG
TCTTGGGTTCAGCATTGCTGGAGGTGTTGGAAACCAGCACATTCCTGGGG
ATAACAGCATCTATGTAACCAAAATAATTGAAGGAGGTGCAGCACACAAG
GACGGCAAACTTCAGATTGGAGACAAGCTTCTAGCAGTGAACAGTGTCTG
TTTAGAAGAAGTTACTCATGAAGAAGCAGTGACTGCCTTAAAGAATACAT
CTGATTTTGTTTATTTGAAAGTGGCAAAACCAACAAGTATGTATATAAAT
GATGGCTATGCACCACCCGACATCACTAATTCTTCTTCTCAGTCTGTTGA
CAACCATGTTAGCCCGTCTTCATACTTGGGCCAGACTCCAGCGTCACCAG
CCAGATACTCACCCATTTCTAAAGCAGTGCTTGGAGATGATGAGATCACT
AGGGAACCTAGAAAAGTTGTTCTTCATCGTGGCTCAACGGGACTTGGTTT
CAACATTGTGGGAGGCGAAGATGGAGAAGGGATTTTTATCTCTTTCATCC
TTGCTGGGGGACCTGCTGACCTAAGCGGAGAGCTCAGAAAAGGAGATCGC
ATTATATCGGTAAACAGTGTTGACCTCAGAGCTGCAAGTCATGAACAAGC
AGCTGCCGCATTAAAAAACGCAGGCCAAGCTGTCACTATTGTCGCACAGT
ATCGGCCTGAAGAGTACAGTCGTTTTGAAGCTAAAATTCACGACTTGCGG
GAGCAGATGATGAACAGCAGTGTCAGCTCAGGGTCAGGGTCTCTCCGAAC
CAGCCAAAAGCGGTCCCTCTATGTCAGAGCCCTTTTTGATTACGACAAGA
CTAAAGACAGTGGCCTTCCCAGTCAAGGGTTGAACTTCAAATTCGGAGAT
ATTCTCCATGTTATCAATGCTTCTGATGACGAGTGGTGGCAGGCCAGACA
GGTGACCCCAGATGGTGAGAGTGACGAAGTTGGAGTAATTCCTAGTAAAC
GCAGAGTTGAGAAGAAAGAACGAGCCCGATTAAAAACAGTCAAATTCAAT
TCTAAAACAAGAGGAGATAAAGGGTCATTCAATGACAAGCGTAAAAAGAA
CCTCTTTTCCCGAAAATTTCCCTTCTACAAGAACAAGGACCAGAGTGAGC
AGGAAACAAGTGATGCTGACCAGCACGTAACTTCTAATGCCAGCGATAGC
GAAAGTAGTTACCGTGGTCAAGAAGAATATGTTTTATCGTATGAGCCGGT
GAATCAGCAAGAAGTTAATTATACCCGACCAGTCATCATATTAGGACCTA
TGAAAGACAGAGTAAATGATGACTTAATCTCAGAATTTCCTGACAAATTT
GGATCCTGTGTCCCTCATACAACTAGACCAAAGCGTGACTATGAGGTGGA
TGGACGAGATTACCATTTTGTGACTTCAAGAGAACAGATGGAGAAAGATA
TCCAGGAGCATAAATTCATTGAAGCTGGCCAGTATAACAACCATCTATAC
GGAACGAGTGTCCAATCTGTGCGAGCAGTAGCAGAGAAGGGCAAGCATTG
TATCCTTGATGTGTCTGGAAATGCCATCAAGAGGTTGCAGATTGCACAGC
TCTATCCAATATCTACTTTTATTAAACCCAAATCCATGGAAAATATCATG
GAAATGAATAAACGCCTAACAGACGAACAGGCCAGAAAAACGTTTGAGAG
AGCAGTGAGACTGGAGCAGGAGTTCACTGAGCATTTCACAGCTATTGTCC
AGGGAGACACGCTGGAGGACATTTACAATCAAGTGAAGCAGATCATAGAA
GAGCAGTCTGGTCCTTACATCTGGGTCCCAGCAAAAGAAAAATTATGACG
CCGGCG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSPLRYPVRKQDTQRALH
LLEEYRSKLNQTEDRQLRSSIERVISIFQSNLFQALIDIQEFYEVTLLDN
PKCVDHSKQCEPVQPGNPWESGSLSSAAVTSESLPGGLSPPVEKYRYQDE
EVLPSERISPQVPNEVLGPELVHVSEKSLSEIENVHGFVSHSHISPIKAN
PPPVLVNTDSLETPTYVNGTDADYEYEEITLERGNSGLGFSIAGGTDNPH
IGDDSSIFITKIITGGAAAQDGRLRVNDCILRVNEADVRDVTHSKAVEAL
KEAGSIVRLYVKRRKPASEKIMEIKLIKGPKGLGFSIAGGVGNQHIPGDN
SIYVTKIIEGGAAHKDGKLQIGDKLLAVNSVCLEEVTHEEAVTALKNTSD
FVYLKVAKPTSMYINDGYAPPDITNSSSQSVDNHVSPSSYLGQTPASPAR
YSPISKAVLGDDEITREPRKVVLHRGSTGLGFNIVGGEDGEGIFISFILA
GGPADLSGELRKGDRIISVNSVDLRAASHEQAAAALKNAGQAVTIVAQYR
PEEYSRFEAKIHDLREQMMNSSVSSGSGSLRTSQKRSLYVRALFDYDKTK
DSGLPSQGLNFKFGDILHVINASDDEWWQARQVTPDGESDEVGVIPSKRR
VEKKERARLKTVKFNSKTRGDKGSFNDKRKKNLFSRKFPFYKNKDQSEQE
TSDADQHVTSNASDSESSYRGQEEYVLSYEPVNQQEVNYTRPVIILGPMK
DRVNDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVTSREQMEKDIQ
EHKFIEAGQYNNHLYGTSVQSVRAVAEKGKHCILDVSGNAIKRLQIAQLY
PISTFIKPKSMENIMEMNKRLTDEQARKTFERAVRLEQEFTEHFTAIVQG
DTLEDIYNQVKQIIEEQSGPYIWVPAKEKL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1858
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.