cDNA Clone PKB

• Synonyms: AKT1
• Plasmid: pFB delta PH PKB alpha S473D
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-delta PH PKB alpha S473D
• Genbank: BC000479
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGACTTCCGGTCGGGCTCACCCAGTGACAACTCAGGGGCTGA
AGAGATGGAGGTGTCCCTGGCCAAGCCCAAGCACCGCGTGACCATGAACG
AGTTTGAGTACCTGAAGCTGCTGGGCAAGGGCACTTTCGGCAAGGTGATC
CTGGTGAAGGAGAAGGCCACAGGCCGCTACTACGCCATGAAGATCCTCAA
GAAGGAAGTCATCGTGGCCAAGGACGAGGTGGCCCACACACTCACCGAGA
ACCGCGTCCTGCAGAACTCCAGGCACCCCTTCCTCACAGCCCTGAAGTAC
TCTTTCCAGACCCACGACCGCCTCTGCTTTGTCATGGAGTACGCCAACGG
GGGCGAGCTGTTCTTCCACCTGTCCCGGGAGCGTGTGTTCTCCGAGGACC
GGGCCCGCTTCTATGGCGCTGAGATTGTGTCAGCCCTGGACTACCTGCAC
TCGGAGAAGAACGTGGTGTACCGGGACCTCAAGCTGGAGAACCTCATGCT
GGACAAGGACGGGCACATTAAGATCACAGACTTCGGGCTGTGCAAGGAGG
GGATCAAGGACGGTGCCACCATGAAGACCTTTTGCGGCACACCTGAGTAC
CTGGCCCCCGAGGTGCTGGAGGACAATGACTACGGCCGTGCAGTGGACTG
GTGGGGGCTGGGCGTGGTCATGTACGAGATGATGTGCGGTCGCCTGCCCT
TCTACAACCAGGACCATGAGAAGCTTTTTGAGCTCATCCTCATGGAGGAG
ATCCGCTTCCCGCGCACGCTTGGTCCCGAGGCCAAGTCCTTGCTTTCAGG
GCTGCTCAAGAAGGACCCCAAGCAGAGGCTTGGCGGGGGCTCCGAGGACG
CCAAGGAGATCATGCAGCATCGCTTCTTTGCCGGTATCGTGTGGCAGCAC
GTGTACGAGAAGAAGCTCAGCCCACCCTTCAAGCCCCAGGTCACGTCGGA
GACTGACACCAGGTATTTTGATGAGGAGTTCACGGCCCAGATGATCACCA
TCACACCACCTGACCAAGATGACAGCATGGAGTGTGTGGACAGCGAGCGC
AGGCCCCACTTCCCCCAGTTCGACTACTCGGCCAGCGGCACGGCCTGA

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMDFRSGSPSDNSGAE
EMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRY
YAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQ
THDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSAL
DYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGA
TMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRL
PFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQ
RLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSET
DTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFDYSA
SGTA

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1850
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for baculovirus expression.