cDNA Clone TPL2

• Synonyms: MAP3K8, COT, EST, ESTF, FLJ10486, MEKK8, TPL2, Tpl-2, c-COT, OTTHUMP00000019392, OTTHUMP00000019393, Ewing sarcoma transformant, cot (cancer Osaka thyroid) oncogene, proto-oncogene c-Cot, proto-oncogene serine/threoine protein kinase, tumor progression locus 2
• Plasmid: pGEX6P-1-TPL2 D1-29
• Parent Plasmid: pGEX6P1
• Expressed: GST-TPL2 D1-29
• Genbank: P41279
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAAAATCTTTATGCAAGTGAAGAGCCAGCAGTTTATGAACC
CAGTCTAATGACCATGTGTCAAGACAGTAATCAAAACGATGAGCGTTCTA
AGTCTCTGCTGCTTAGTGGCCAAGAGGTACCATGGTTGTCATCAGTCAGA
TATGGAACTGTGGAGGATTTGCTTGCTTTTGCAAACCATATATCCAACAC
TGCAAAGCATTTTTATGGACAACGACCACAGGAATCTGGAATTTTATTAA
ACATGGTCATCACTCCCCAAAATGGACGTTACCAAATAGATTCCGATGTT
CTCCTGATCCCCTGGAAGCTGACTTACAGGAATATTGGTTCTGATTTTAT
TCCTCGGGGCGCCTTTGGAAAGGTATACTTGGCTCAAGATATAAAGACGA
AGAAAAGAATGGCGTGTAAACTGATCCCAGTAGATCAATTTAAGCCATCT
GATGTGGAAATTCAGGCTTGCTTCCGGCACGAGAACATCGCAGAGCTGTA
TGGCGCAGTCCTGTGGGGTGAAACTGTCCATCTCTTTATGGAAGCAGGCG
AGGGAGGGTCTGTTCTGGAGAAACTGGAGAGCTGTGGACCAATGAGAGAA
TTTGAAATTATTTGGGTGACAAAGCATGTTCTCAAGGGACTTGATTTTCT
ACACTCAAAGAAAGTGATCCATCATGATATTAAACCTAGCAACATTGTTT
TCATGTCCACAAAAGCTGTTTTGGTGGATTTTGGCCTAAGTGTTCAAATG
ACCGAAGATGTCTATTTTCCTAAGGACCTCCGAGGAACAGAGATTTACAT
GAGCCCAGAGGTCATCCTGTGCAGGGGCCATTCAACCAAAGCAGACATCT
ACAGCCTGGGGGCCACGCTCATCCACATGCAGACGGGCACCCCACCCTGG
GTGAAGCGCTACCCTCGCTCAGCCTATCCCTCCTACCTGTACATAATCCA
CAAGCAAGCACCTCCACTGGAAGACATTGCAGATGACTGCAGTCCAGGGA
TGAGAGAGCTGATAGAAGCTTCCCTGGAGAGAAACCCCAATCACCGCCCA
AGAGCCGCAGACCTACTAAAACATGAGGCCCTGAACCCGCCCAGAGAGGA
TCAGCCACGCTGTACGAGTCTGGACTCTGCCCTCTTGGAGCGCAAGAGGC
TGCTGAGTAGGAAGGAGCTGGAACTTCCTGAGAACATTGCTGATTCTTCG
TGCACAGGAAGCACCGAGGAATCTGAGATGCTCAAGAGGCAACGCTCTCT
CTACATCGACCTCGGCGCTCTGGCTGGCTACTTCAATCTTGTTCGGGGAC
CACCAACGCTTGAATATGGCTGAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMENLYASEEPAVYEPSLMT
MCQDSNQNDERSKSLLLSGQEVPWLSSVRYGTVEDLLAFANHISNTAKHF
YGQRPQESGILLNMVITPQNGRYQIDSDVLLIPWKLTYRNIGSDFIPRGA
FGKVYLAQDIKTKKRMACKLIPVDQFKPSDVEIQACFRHENIAELYGAVL
WGETVHLFMEAGEGGSVLEKLESCGPMREFEIIWVTKHVLKGLDFLHSKK
VIHHDIKPSNIVFMSTKAVLVDFGLSVQMTEDVYFPKDLRGTEIYMSPEV
ILCRGHSTKADIYSLGATLIHMQTGTPPWVKRYPRSAYPSYLYIIHKQAP
PLEDIADDCSPGMRELIEASLERNPNHRPRAADLLKHEALNPPREDQPRC
TSLDSALLERKRLLSRKELELPENIADSSCTGSTEESEMLKRQRSLYIDL
GALAGYFNLVRGPPTLEYG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1812
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GSTtag