cDNA Clone TOPK

• Plasmid: pCMV5-HA TOPK
• Parent Plasmid: pCMV5
• Expressed: HA-TOPK
• Genbank: AAF69107
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGAAGGGAT
CAGTAATTTCAAGACACCAAGCAAATTATCAGAAAAAAAGAAATCTGTAT
TATGTTCAACTCCAACTATAAATATCCCGGCCTCTCCATTTATGCAGAAG
CTTGGCTTTGGTACTGGGGTAAATGTGTACCTAATGAAAAGATCTCCAAG
AGGTTTGTCTCATTCTCCTTGGGCTGTAAAAAAGATTAATCCTATATGTA
ATGATCATTATCGAAGTGTGTATCAAAAGAGACTAATGGATGAAGCTAAG
ATTTTGAAAAGCCTTCATCATCCAAACATTGTTGGTTATCGTGCTTTTAC
TGAAGCCAATGATGGCAGTCTGTGTCTTGCTATGGAATATGGAGGTGAAA
AGTCTCTAAATGACTTAATAGAAGAACGATATAAAGCCAGCCAAGATCCT
TTTCCAGCAGCCATAATTTTAAAAGTTGCTTTGAATATGGCAAGAGGGTT
AAAGTATCTGCACCAAGAAAAGAAACTGCTTCATGGAGACATAAAGTCTT
CAAATGTTGTAATTAAAGGCGATTTTGAAACAATTAAAATCTGTGATGTA
GGAGTCTCTCTACCACTGGATGAAAATATGACTGTGACTGACCCTGAGGC
TTGTTACATTGGCACAGAGCCATGGAAACCCAAAGAAGCTGTGGAGGAGA
ATGGTGTTATTACTGACAAGGCAGACATATTTGCCTTTGGCCTTACTTTG
TGGGAAATGATGACTTTATCGATTCCACACATTAATCTTTCAAATGATGA
TGATGATGAAGATAAAACTTTTGATGAAAGTGATTTTGATGATGAAGCAT
ACTATGCAGCGTTGGGAACTAGGCCACCTATTAATATGGAAGAACTGGAT
GAATCATACCAGAAAGTAATTGAACTCTTCTCTGTATGCACTAATGAAGA
CCCTAAAGATCGTCCTTCTGCTGCACACATTGTTGAAGCTCTGGAAACAG
ATGTCTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAEGISNFKTPSKLSEKKKSVLCSTPTINIPASPFMQKLGFG
TGVNVYLMKRSPRGLSHSPWAVKKINPICNDHYRSVYQKRLMDEAKILKS
LHHPNIVGYRAFTEANDGSLCLAMEYGGEKSLNDLIEERYKASQDPFPAA
IILKVALNMARGLKYLHQEKKLLHGDIKSSNVVIKGDFETIKICDVGVSL
PLDENMTVTDPEACYIGTEPWKPKEAVEENGVITDKADIFAFGLTLWEMM
TLSIPHINLSNDDDDEDKTFDESDFDDEAYYAALGTRPPINMEELDESYQ
KVIELFSVCTNEDPKDRPSAAHIVEALETDV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1801
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression.; Estimated MW of expressed protein : 37167 Daltons