cDNA Clone ROCK1 (rat)

• Plasmid: pFBHTb HA ROCK1 delta 544-1380
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-HA ROCK1 delta 544-1380
• Genbank: NM_013022
• Tag: 6His
• Species: Rat

Sequence of Insert

GGATCCGAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCC
CGGCGCCCCCGAGGCCGCGCCGGGGGACGGGGCGGGCGCGGGTCGTCAGA
GGAAGCTGGAGGCGCTCATCCGAGACCCTCGCTCGCCCATCAACGTGGAA
AGCCTGCTGGATGGCTTAAATTCCTTGGTCCTTGATTTGGATTTTCCTGC
TTTGAGGAAAAATAAAAATATAGATAATTTCCTAAATAGATATGAGAAAA
TTGTGAAAAAAATCAGAGGTTTACAGATGAAAGCAGAAGACTATGATGTT
GTAAAAGTTATTGGAAGAGGTGCTTTTGGTGAAGTTCAGTTGGTTCGTCA
TAAGGCATCACAGAAGGTTTATGCAATGAAGCTTCTTAGTAAATTTGAAA
TGATAAAAAGATCAGATTCTGCTTTTTTCTGGGAAGAAAGAGATATTATG
GCTTTTGCTAACAGTCCGTGGGTGGTTCAGCTCTTTTGTGCCTTTCAAGA
TGACAGGTATCTGTACATGGTGATGGAGTACATGCCAGGTGGAGACCTTG
TTAACCTGATGAGCAATTATGATGTGCCTGAGAAGTGGGCCAAGTTTTAT
ACTGCTGAAGTCGTGCTTGCACTGGATGCAATACACTCCATGGGCTTAAT
ACACAGAGATGTGAAGCCCGATAACATGCTATTGGATAAACACGGACATC
TAAAATTAGCAGATTTTGGCACATGTATGAAAATGGATGAAACAGGCATG
GTGCATTGTGATACAGCAGTTGGAACTCCAGACTACATATCACCTGAGGT
TCTGAAATCACAAGGAGGTGATGGTTACTATGGACGAGAATGTGATTGGT
GGTCTGTAGGTGTTTTCCTTTTTGAGATGTTAGTAGGGGACACTCCATTT
TATGCAGATTCACTTGTAGGAACCTACAGCAAAATTATGGATCATAAAAA
TTCACTGTGTTTTCCTGAAGATACAGAAATTTCTAAACATGCGAAGAATC
TCATATGTGCCTTCTTAACAGACAGGGAGGTACGACTTGGAAGAAATGGG
GTAGAAGAAATCAAATCAGCATCCTTCTTTAAGAATGATCAGTGGAATTG
GGATAACATAAGAGAGACGGCAGCTCCAGTGGTGCCTGAGCTCAGCAGTG
ACATAGACAGCAGCAACTTTGATGACATTGAGGATGACAAAGGCGATGTA
GAGACCTTCCCGATTCCTAAAGCTTTTGTGGGAAATCAGCTGCCTTTTAT
AGGATTTACCTACTTTAGAGAAAATTTGTTACTAAGTGATTCTCCACCTT
GTAGAGAAAATGATGCAATACAAACAAGGAAAAGTGAAGAAAGTCAGGAG
ATTCAGAAAAAATTATATGCACTAGAAGAACACCTTAGCAGTGAGGTACA
AGCCAAGGAGGAACTGGAACAGAAGTGCAAGTCTATTAATACTCGTCTAG
AGAAAACAGCAAAGGAACTAGAAGAAGAGATTACCTTTAGGAAAAATGTG
GAATCAACATTAAGACAGCTAGAAAGAGAAAAGGCCCTTCTTCAGCACAA
AAATGCAGAATATCAACGGAAAGCTGATCATGAAGCTGACAAGAAACGGA
ATTTGGAAAATGATGTTAACAGCTTAAAAGATCAACTTGAAGATTTGAAG
AAAAGAAACCAGAGCTCTCAGATATAAGGATCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSEFATMYPYDVPDYAPGAPEAAP
GDGAGAGRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNI
DNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVY
AMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDRYLYMV
MEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPD
NMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGD
GYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPED
TEISKHAKNLICAFLTDREVRLGRNGVEEIKSASFFKNDQWNWDNIRETA
APVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYFRE
NLLLSDSPPCRENDAIQTRKSEESQEIQKKLYALEEHLSSEVQAKEELEQ
KCKSINTRLEKTAKELEEEITFRKNVESTLRQLEREKALLQHKNAEYQRK
ADHEADKKRNLENDVNSLKDQLEDLKKRNQSSQI*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1797
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His tag for baculovirus expression.