cDNA Clone SAP97 (rat)

• Plasmid: pEBG-2T-SAP97 S442A
• Parent Plasmid: pEBG2T
• Expressed: GST-SAP97 S442A
• Genbank: AM710296.2
• Tag: GST
• Species: Rat

Sequence of Insert

GGTACCATGCCGGTCCGGAAGCAAGATACCCAGAGAGCATTGCATCTGTT
GGAAGAATATCGGTCGAAACTAAACCAAACCGAAGACAGACAGCTCAGGA
GTTCCATAGAGCGGGTTATTAGCATATTTCAGAGCAACCTCTTTCAGGCT
TTAATAGACATTCAAGAATTTTATGAAGTGACCTTACTTGATAATCCAAA
ATGTGTGGATCATTCAAAGCAGTGTGAACCGGTCCAACCCGGGAATCCTT
GGGAGAGTGGCAGCCTTTCAAGTGCTGCTGTGACTTCAGAGAGCCTGCCC
GGCGGCCTTAGCCCTCCAGTAGAGAAATACCGGTATCAGGACGAAGAGGT
GCTGCCTTCAGAGCGGATTTCCCCGCAAGTCCCAAATGAGGTGCTGGGTC
CGGAGCTGGTTCACGTCTCGGAGAAGAGCCTGTCAGAGATTGAGAATGTC
CACGGGTTTGTCTCTCACTCTCATATCTCACCCATAAAGGCAAATCCTCC
TCCTGTGCTGGTCAACACAGACAGCTTGGAGACACCAACTTATGTTAATG
GTACTGATGCAGATTATGAATATGAGGAAATCACACTTGAAAGGGGAAAT
TCAGGCCTTGGTTTCAGCATTGCAGGAGGTACAGACAACCCACACATTGG
AGATGACTCAAGTATTTTCATCACCAAAATTATCACGGGAGGAGCAGCTG
CCCAGGATGGAAGATTGCGGGTAAATGACTGTATACTGAGAGTAAATGAA
GCAGATGTTCGTGATGTAACCCACAGCAAAGCAGTTGAAGCATTGAAAGA
AGCTGGATCGATAGTGCGATTATATGTGAAAAGGCGGAAGCCAGCCTCAG
AAAAAATCATGGAAATAAAACTTATTAAAGGCCCTAAAGGTCTTGGGTTC
AGCATTGCTGGAGGTGTTGGAAACCAGCACATTCCTGGGGATAACAGCAT
CTATGTAACCAAAATAATTGAAGGAGGTGCAGCACACAAGGACGGCAAAC
TTCAGATTGGAGACAAGCTTCTAGCAGTGAACAGTGTCTGTTTAGAAGAA
GTTACTCATGAAGAAGCAGTGACTGCCTTAAAGAATACATCTGATTTTGT
TTATTTGAAAGTGGCAAAACCAACAAGTATGTATATAAATGATGGCTATG
CACCACCCGACATCACTAATTCTTCTTCTCAGTCTGTTGACAACCATGTT
AGCCCGTCTTCATACTTGGGCCAGACTCCAGCGGCACCAGCCAGATACTC
ACCCATTTCTAAAGCAGTGCTTGGAGATGATGAGATCACTAGGGAACCTA
GAAAAGTTGTTCTTCATCGTGGCTCAACGGGACTTGGTTTCAACATTGTG
GGAGGCGAAGATGGAGAAGGGATTTTTATCTCTTTCATCCTTGCTGGGGG
ACCTGCTGACCTAAGCGGAGAGCTCAGAAAAGGAGATCGCATTATATCGG
TAAACAGTGTTGACCTCAGAGCTGCAAGTCATGAACAAGCAGCTGCCGCA
TTAAAAAACGCAGGCCAAGCTGTCACTATTGTCGCACAGTATCGGCCTGA
AGAGTACAGTCGTTTTGAAGCTAAAATTCACGACTTGCGGGAGCAGATGA
TGAACAGCAGTGTCAGCTCAGGGTCAGGGTCTCTCCGAACCAGCCAAAAG
CGGTCCCTCTATGTCAGAGCCCTTTTTGATTACGACAAGACTAAAGACAG
TGGCCTTCCCAGTCAAGGGTTGAACTTCAAATTCGGAGATATTCTCCATG
TTATCAATGCTTCTGATGACGAGTGGTGGCAGGCCAGACAGGTGACCCCA
GATGGTGAGAGTGACGAAGTTGGAGTAATTCCTAGTAAACGCAGAGTTGA
GAAGAAAGAACGAGCCCGATTAAAAACAGTCAAATTCAATTCTAAAACAA
GAGGAGATAAAGGGTCATTCAATGACAAGCGTAAAAAGAACCTCTTTTCC
CGAAAATTTCCCTTCTACAAGAACAAGGACCAGAGTGAGCAGGAAACAAG
TGATGCTGACCAGCACGTAACTTCTAATGCCAGCGATAGCGAAAGTAGTT
ACCGTGGTCAAGAAGAATATGTTTTATCGTATGAGCCGGTGAATCAGCAA
GAAGTTAATTATACCCGACCAGTCATCATATTAGGACCTATGAAAGACAG
AGTAAATGATGACTTAATCTCAGAATTTCCTGACAAATTTGGATCCTGTG
TCCCTCATACAACTAGACCAAAGCGTGACTATGAGGTGGATGGACGAGAT
TACCATTTTGTGACTTCAAGAGAACAGATGGAGAAAGATATCCAGGAGCA
TAAATTCATTGAAGCTGGCCAGTATAACAACCATCTATACGGAACGAGTG
TCCAATCTGTGCGAGCAGTAGCAGAGAAGGGCAAGCATTGTATCCTTGAT
GTGTCTGGAAATGCCATCAAGAGGTTGCAGATTGCACAGCTCTATCCAAT
ATCTACTTTTATTAAACCCAAATCCATGGAAAATATCATGGAAATGAATA
AACGCCTAACAGACGAACAGGCCAGAAAAACGTTTGAGAGAGCAGTGAGA
CTGGAGCAGGAGTTCACTGAGCATTTCACAGCTATTGTCCAGGGAGACAC
GCTGGAGGACATTTACAATCAAGTGAAGCAGATCATAGAAGAGCAGTCTG
GTCCTTACATCTGGGTCCCAGCAAAAGAAAAATTATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSTSGTIDAAASVMDICRIRPCGTPV
RKQDTQRALHLLEEYRSKLNQTEDRQLRSSIERVISIFQSNLFQALIDIQ
EFYEVTLLDNPKCVDHSKQCEPVQPGNPWESGSLSSAAVTSESLPGGLSP
PVEKYRYQDEEVLPSERISPQVPNEVLGPELVHVSEKSLSEIENVHGFVS
HSHISPIKANPPPVLVNTDSLETPTYVNGTDADYEYEEITLERGNSGLGF
SIAGGTDNPHIGDDSSIFITKIITGGAAAQDGRLRVNDCILRVNEADVRD
VTHSKAVEALKEAGSIVRLYVKRRKPASEKIMEIKLIKGPKGLGFSIAGG
VGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAVNSVCLEEVTHEE
AVTALKNTSDFVYLKVAKPTSMYINDGYAPPDITNSSSQSVDNHVSPSSY
LGQTPAAPARYSPISKAVLGDDEITREPRKVVLHRGSTGLGFNIVGGEDG
EGIFISFILAGGPADLSGELRKGDRIISVNSVDLRAASHEQAAAALKNAG
QAVTIVAQYRPEEYSRFEAKIHDLREQMMNSSVSSGSGSLRTSQKRSLYV
RALFDYDKTKDSGLPSQGLNFKFGDILHVINASDDEWWQARQVTPDGESD
EVGVIPSKRRVEKKERARLKTVKFNSKTRGDKGSFNDKRKKNLFSRKFPF
YKNKDQSEQETSDADQHVTSNASDSESSYRGQEEYVLSYEPVNQQEVNYT
RPVIILGPMKDRVNDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVT
SREQMEKDIQEHKFIEAGQYNNHLYGTSVQSVRAVAEKGKHCILDVSGNA
IKRLQIAQLYPISTFIKPKSMENIMEMNKRLTDEQARKTFERAVRLEQEF
TEHFTAIVQGDTLEDIYNQVKQIIEEQSGPYIWVPAKEKL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU1772
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for mammalian expression. n.b. The current genbank reference differs to the expressed protein.
• Low Temp: No