cDNA Clone 14-3-3 zeta

• Synonyms: YWHAZ
• Plasmid: pETBlue 6His 14-3-3 zeta
• Parent Plasmid: pETBlue2 original
• Expressed: 6His 14-3-3 zeta
• Genbank: NM_145690
• Tag: 6His
• Species: Human

Sequence of Insert

CCATGGGACACCATCACCATCACCATCTGGAAGTTCTGTTCCAGGGGCCC
GATAAAAATGAGCTGGTTCAGAAGGCCAAACTGGCCGAGCAGGCTGAGCG
ATATGATGACATGGCAGCCTGCATGAAGTCTGTAACTGAGCAAGGAGCTG
AATTATCCAATGAGGAGAGGAATCTTCTCTCAGTTGCTTATAAAAATGTT
GTAGGAGCCCGTAGGTCATCTTGGAGGGTCGTCTCAAGTATTGAACAAAA
GACGGAAGGTGCTGAGAAAAAACAGCAGATGGCTCGAGAATACAGAGAGA
AAATTGAGACGGAGCTAAGAGATATCTGCAATGATGTACTGTCTCTTTTG
GAAAAGTTCTTGATCCCCAATGCTTCACAAGCAGAGAGCAAAGTCTTCTA
TTTGAAAATGAAAGGAGATTACTACCGTTACTTGGCTGAGGTTGCCGCTG
GTGATGACAAGAAAGGGATTGTCGATCAGTCACAACAAGCATACCAAGAA
GCTTTTGAAATCAGCAAAAAGGAAATGCAACCAACACATCCTATCAGACT
GGGTCTGGCCCTTAACTTCTCTGTGTTCTATTATGAGATTCTGAACTCCC
CAGAGAAAGCCTGCTCTCTTGCAAAGACAGCTTTTGATGAAGCCATTGCT
GAACTTGATACATTAAGTGAAGAGTCATACAAAGACAGCACGCTAATAAT
GCAATTACTGAGAGACAACTTGACATTGTGGACATCGGATACCCAAGGAG
ACGAAGCTGAAGCAGGAGAAGGAGGGGAAAATTAAGGATCC

Amino Acid Sequence

MGHHHHHHLEVLFQGPDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAE
LSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREK
IETELRDICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAG
DDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSP
EKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGD
EAEAGEGGEN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1762
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His tag for bacterial expression with precission protease site.