cDNA Clone SGK1

• Plasmid: pFB HTc SGK1delta N S422D
• Parent Plasmid: pFastbac HTc
• Expressed: 6His-SGK1delta N S422D
• Genbank: O00141
• Tag: 6His
• Species: Human

Sequence of Insert

AGATCTCCCAACCTCAGGAGCCTGAGCTTATGAATGCCAACCCTTCTCCT
CCACCAAGTCCTTCTCAGCAAATCAACCTTGGCCCGTCGTCCAATCCTCA
TGCTAAACCATCTGACTTTCACTTCTTGAAAGTGATCGGAAAGGGCAGTT
TTGGAAAGGTTCTTCTAGCAAGACACAAGGCAGAAGAAGTGTTCTATGCA
GTCAAAGTTTTACAGAAGAAAGCAATCCTGAAAAAGAAAGAGGAGAAGCA
TATTATGTCGGAGCGGAATGTTCTGTTGAAGAATGTGAAGCACCCTTTCC
TGGTGGGCCTTCACTTCTCTTTCCAGACTGCTGACAAATTGTACTTTGTC
CTAGACTACATTAATGGTGGAGAGTTGTTCTACCATCTCCAGAGGGAACG
CTGCTTCCTGGAACCACGGGCTCGTTTCTATGCTGCTGAAATAGCCAGTG
CCTTGGGCTACCTGCATTCACTGAACATCGTTTATAGAGACTTAAAACCA
GAGAATATTTTGCTAGATTCACAGGGACACATTGTCCTTACTGACTTCGG
ACTCTGCAAGGAGAACATTGAACACAACAGCACAACATCCACCTTCTGTG
GCACGCCGGAGTATCTCGCACCTGAGGTGCTTCATAAGCAGCCTTATGAC
AGGACTGTGGACTGGTGGTGCCTGGGAGCTGTCTTGTATGAGATGCTGTA
TGGCCTGCCGCCTTTTTATAGCCGAAACACAGCTGAAATGTACGACAACA
TTCTGAACAAGCCTCTCCAGCTGAAACCAAATATTACAAATTCCGCAAGA
CACCTCCTGGAGGGCCTCCTGCAGAAGGACAGGACAAAGCGGCTCGGGGC
CAAGGATGACTTCATGGAGATTAAGAGTCATGTCTTCTTCTCCTTAATTA
ACTGGGATGATCTCATTAATAAGAAGATTACTCCCCCTTTTAACCCAAAT
GTGAGTGGGCCCAACGACCTACGGCACTTTGACCCCGAGTTTACCGAAGA
GCCTGTCCCCAACTCCATTGGCAAGTCCCCTGACAGCGTCCTCGTCACAG
CCAGCGTCAAGGAAGCTGCCGAGGCTTTCCTAGGCTTTGACTATGCGCCT
CCCACGGACTCTTTCCTCTGAGTCGAC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGISQPQEPELMNANPSPPPSPSQQ
INLGPSSNPHAKPSDFHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKK
AILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGG
ELFYHLQRERCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDS
QGHIVLTDFGLCKENIEHNSTTSTFCGTPEYLAPEVLHKQPYDRTVDWWC
LGAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLL
QKDRTKRLGAKDDFMEIKSHVFFSLINWDDLINKKITPPFNPNVSGPNDL
RHFDPEFTEEPVPNSIGKSPDSVLVTASVKEAAEAFLGFDYAPPTDSFL

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1737
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express 6His N terminal fusion