cDNA Clone TAK1-S

• Plasmid: pFB 6His-TAK1
• Parent Plasmid: pFastbac1
• Expressed: 6His-TAK1
• Genbank: NP_003179
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCACCATGCACCATCACCATCACCATATGTCTACAGCCTCTGCCGCC
TCCTCCTCCTCCTCGTCTTCGGCCGGTGAGATGATCGAAGCCCCTTCCCA
GGTCCTCAACTTTGAAGAGATCGACTACAAGGAGATCGAGGTGGAAGAGG
TTGTTGGAAGAGGAGCCTTTGGAGTTGTTTGCAAAGCTAAGTGGAGAGCA
AAAGATGTTGCTATTAAACAAATAGAAAGTGAATCTGAGAGGAAAGCGTT
TATTGTAGAGCTTCGGCAGTTATCCCGTGTGAACCATCCTAATATTGTAA
AGCTTTATGGAGCCTGCTTGAATCCAGTGTGTCTTGTGATGGAATATGCT
GAAGGGGGCTCTTTATATAATGTGCTGCATGGTGCTGAACCATTGCCATA
TTATACTGCTGCCCACGCAATGAGTTGGTGTTTACAGTGTTCCCAAGGAG
TGGCTTATCTTCACAGCATGCAACCCAAAGCGCTAATTCACAGGGACCTG
AAACCACCAAACTTACTGCTGGTTGCAGGGGGGACAGTTCTAAAAATTTG
TGATTTTGGTACAGCCTGTGACATTCAGACACACATGACCAATAACAAGG
GGAGTGCTGCTTGGATGGCACCTGAAGTTTTTGAAGGTAGTAATTACAGT
GAAAAATGTGACGTCTTCAGCTGGGGTATTATTCTTTGGGAAGTGATAAC
GCGTCGGAAACCCTTTGATGAGATTGGTGGCCCAGCTTTCCGAATCATGT
GGGCTGTTCATAATGGTACTCGACCACCACTGATAAAAAATTTACCTAAG
CCCATTGAGAGCCTGATGACTCGTTGTTGGTCTAAAGATCCTTCCCAGCG
CCCTTCAATGGAGGAAATTGTGAAAATAATGACTCACTTGATGCGGTACT
TTCCAGGAGCAGATGAGCCATTACAGTATCCTTGTCAGTATTCAGATGAA
GGACAGAGCAACTCTGCCACCAGTACAGGCTCATTCATGGACATTGCTTC
TACAAATACGAGTAACAAAAGTGACACTAATATGGAGCAAGTTCCTGCCA
CAAATGATACTATTAAGCGCTTAGAATCAAAATTGTTGAAAAATCAGGCA
AAGCAACAGAGTGAATCTGGACGTTTAAGCTTGGGAGCCTCCCGTGGGAG
CAGTGTGGAGAGCTTGCCCCCAACCTCTGAGGGCAAGAGGATGAGTGCTG
ACATGTCTGAAATAGAAGCTAGGATCGCCGCAACCACAGGCAACGGACAG
CCAAGACGTAGATCCATCCAAGACTTGACTGTAACTGGAACAGAACCTGG
TCAGGTGAGCAGTAGGTCATCCAGTCCCAGTGTCAGAATGATTACTACCT
CAGGACCAACCTCAGAAAAGCCAACTCGAAGTCATCCATGGACCCCTGAT
GATTCCACAGATACCAATGGATCAGATAACTCCATCCCAATGGCTTATCT
TACACTGGATCACCAACTACAGCCTCTAGCACCGTGCCCAAACTCCAAAG
AATCTATGGCAGTGTTTGAACAGCATTGTAAAATGGCACAAGAATATATG
AAAGTTCAAACAGAAATTGCATTGTTATTACAGAGAAAGCAAGAACTAGT
TGCAGAACTGGACCAGGATGAAAAGGACCAGCAAAATACATCTCGCCTGG
TACAGGAACATAAAAAGCTTTTAGATGAAAACAAAAGCCTTTCTACTTAC
TACCAGCAATGCAAAAAACAACTAGAGGTCATCAGAAGTCAGCAGCAGAA
ACGACAAGGCACTTCATGAGGATCC

Amino Acid Sequence

MHHHHHHMSTASAASSSSSSSAGEMIEAPSQVLNFEEIDYKEIEVEEVVG
RGAFGVVCKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLY
GACLNPVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAY
LHSMQPKALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSA
AWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAV
HNGTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYFPG
ADEPLQYPCQYSDEGQSNSATSTGSFMDIASTNTSNKSDTNMEQVPATND
TIKRLESKLLKNQAKQQSESGRLSLGASRGSSVESLPPTSEGKRMSADMS
EIEARIAATTGNGQPRRRSIQDLTVTGTEPGQVSSRSSSPSVRMITTSGP
TSEKPTRSHPWTPDDSTDTNGSDNSIPMAYLTLDHQLQPLAPCPNSKESM
AVFEQHCKMAQEYMKVQTEIALLLQRKQELVAELDQDEKDQQNTSRLVQE
HKKLLDENKSLSTYYQQCKKQLEVIRSQQQKRQGTS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1689
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for baculovirus expression expression