cDNA Clone TPS5

• Plasmid: pGEX6P-1-TPS5 delta N
• Parent Plasmid: pGEX6P1
• Genbank: NM_117886
• Species: Arabidopsis thaliana

Sequence of Insert

GGATCCATGGTATCAAGATCTTATTCAAACCTCTTGGATCTTGCTTCTGG
TAACTTCCATTCGTTTTCTCGAGAAAAGAAGAGGTTTCCAAGAGTAGCAA
CTGTCACTGGTGTCTTATCTGAGCTAGATGATGATAACAACAGCAACAGT
GTCTGCTCTGATGCTCCTTCTTCCGTCACACAAGATCGAATCATCATCGT
TGGAAACCAGCTTCCTATTAAATCACATCGGAACTCTGCTGGTAAATTGA
GTTTTAGTTGGGACAATGACTCGCTTCTCTTGCAGCTTAAAGATGGTATG
CGTGAAGATATGGAAGTTGTCTACATTGGTTGCCTTAAGGAACAGATTGA
TACAGTTGAGCAAGATGATGTTTCTCAAAGGTTGCTTGAGAATTTCAAAT
GTGTTCCTGCTTATATCCCACCTGAGCTATTCACTAAGTACTATCATGGA
TTCTGTAAGCAACATCTATGGCCTTTGTTTCACTACATGCTTCCCTTAAC
TCCTGATCTTGGTGGTAGATTTGATCGTTCTTTATGGCAAGCTTATCTTT
CGGTTAACAAGATCTTTGCTGATAAAGTGATGGAAGTGATTAGTCCTGAT
GATGATTTTGTTTGGGTTCATGACTATCACTTAATGGTTTTGCCTACATT
TCTAAGGAAGAGGTTTAATAGAGTAAAGCTTGGTTTTTTCCTTCATAGCC
CGTTCCCTTCCTCTGAGATTTACCGTACTCTTCCAGTGAGAAATGAGCTC
TTACGTGCACTCCTCAACGCTGATTTGATTGGCTTTCATACCTTTGACTA
TGCAAGACACTTCCTTTCTTGTTGTAGCAGGATGCTTGGTTTATCCTATC
AGTCCAAACGTGGAACCATAGGGCTTGAGTATTATGGTCGAACGGTTAGT
ATCAAGATTCTTCCTGTGGGGATTCATATCAGCCAGCTTCAGTCAATTTT
AAACCTCCCAGAGACTCAGACCAAAGTTGCTGAGCTAAGAGATCAGTTCT
TGGATCAGAAAGTTCTTCTCGGTGTTGATGACATGGACATCTTCAAAGGA
ATCAGCCTCAAACTCTTGGCAATGGAACAACTTCTCACACAGCATCCCGA
GAAGAGAGGTCGAGTTGTACTTGTCCAGATTGCAAACCCTGCAAGAGGTC
GTGGGAAAGACGTTCAGGAGGTTCAGTCTGAAACTGAAGCCACGGTTAAA
AGGATCAATGAAATGTTTGGAAGGCCGGGCTACCAACCCGTGGTTCTGAT
TGATACACCGCTTCAATTCTTTGAGAGGATTGCTTACTATGTCATTGCAG
AGTGTTGTCTTGTTACAGCGGTAAGAGATGGTATGAATCTTATACCTTAT
GAGTACATTATCTGCAGGCAAGGTAATCCGAAACTCAACGAGACTATAGG
CCTTGACCCTTCTGCTGCAAAGAAGAGTATGCTTGTTGTCTCTGAGTTTA
TTGGTTGTTCTCCTTCTTTAAGCGGCGCCATTAGAGTAAATCCGTGGAAC
ATTGATGCTGTGACTGAAGCAATGGACTATGCATTGATAGTTTCAGAAGC
AGAGAAGCAAATGCGTCACGAGAAGCATCACAAATATGTTAGCACACATG
ATGTTGCTTATTGGGCGCGTAGCTTTATACAAGATCTTGAAAGGGCTTGC
GGGGATCATGTGAGGAAGAGGTGTTGGGGGATTGGATTCGGGTTAGGCTT
TAGAGTTGTGGCGCTTGATCCGAGTTTTAAAAAGCTTTCGATTGAGCACA
TTGTCTCAGCTTATAAGAGAACCAAGAACCGAGCCATTTTGTTGGATTAT
GATGGCACAATGGTGCAGCCAGGTTCCATTAGGACAACACCAACCCGCGA
AACAATCGAAATCTTGAACAACCTGTCTAGTGATCCCAAGAATATCGTGT
ACCTCGTCAGTGGGAAAGACAGAAGGACACTAACTGAATGGTTTTCTTCA
TGTGACGATCTCGGTTTGGGTGCAGAGCACGGGTATTTTATAAGGCCAAA
TGATGGAACAGACTGGGAAACGTCGAGTTTGGTATCAGGTTTTGAGTGGA
AACAAATAGCAGAGCCAGTGATGAGACTTTACACTGAGACTACAGATGGA
TCAACAATAGAGACTAAAGAGACTGCTCTCGTTTGGAATTACCAATTCGC
AGATCCTGATTTTGGATCTTGTCAAGCCAAAGAGCTTATGGAACACCTCG
AAAGCGTGCTTACCAATGATCCAGTCTCTGTCAAGACTGGACAACAACTC
GTTGAAGTTAAACCACAGGGTGTGAACAAAGGTCTTGTGGCAGAGAGGCT
TCTAACAACGATGCAAGAAAAAGGGAAACTTTTGGATTTCATTCTCTGCG
TCGGTGATGATCGGTCTGATGAGGATATGTTTGAGGTGATAATGAGTGCT
AAAGATGGTCCAGCTTTGTCTCCTGTGGCTGAGATATTCGCTTGCACCGT
TGGACAAAAGCCAAGCAAAGCAAAATACTATTTAGACGATACTGCGGAGA
TAATCAGAATGCTGGACGGTCTAGCCGCTACCAACACAACTATCTCTGAT
CAAACTGATTCAACCGCCACTGTTCCAACTAAAGATCTGTTTTAAGAATT
CGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMVSRSYSNLLDLASGNFHS
FSREKKRFPRVATVTGVLSELDDDNNSNSVCSDAPSSVTQDRIIIVGNQL
PIKSHRNSAGKLSFSWDNDSLLLQLKDGMREDMEVVYIGCLKEQIDTVEQ
DDVSQRLLENFKCVPAYIPPELFTKYYHGFCKQHLWPLFHYMLPLTPDLG
GRFDRSLWQAYLSVNKIFADKVMEVISPDDDFVWVHDYHLMVLPTFLRKR
FNRVKLGFFLHSPFPSSEIYRTLPVRNELLRALLNADLIGFHTFDYARHF
LSCCSRMLGLSYQSKRGTIGLEYYGRTVSIKILPVGIHISQLQSILNLPE
TQTKVAELRDQFLDQKVLLGVDDMDIFKGISLKLLAMEQLLTQHPEKRGR
VVLVQIANPARGRGKDVQEVQSETEATVKRINEMFGRPGYQPVVLIDTPL
QFFERIAYYVIAECCLVTAVRDGMNLIPYEYIICRQGNPKLNETIGLDPS
AAKKSMLVVSEFIGCSPSLSGAIRVNPWNIDAVTEAMDYALIVSEAEKQM
RHEKHHKYVSTHDVAYWARSFIQDLERACGDHVRKRCWGIGFGLGFRVVA
LDPSFKKLSIEHIVSAYKRTKNRAILLDYDGTMVQPGSIRTTPTRETIEI
LNNLSSDPKNIVYLVSGKDRRTLTEWFSSCDDLGLGAEHGYFIRPNDGTD
WETSSLVSGFEWKQIAEPVMRLYTETTDGSTIETKETALVWNYQFADPDF
GSCQAKELMEHLESVLTNDPVSVKTGQQLVEVKPQGVNKGLVAERLLTTM
QEKGKLLDFILCVGDDRSDEDMFEVIMSAKDGPALSPVAEIFACTVGQKP
SKAKYYLDDTAEIIRMLDGLAATNTTISDQTDSTATVPTKDLF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1679
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag.
• Low Temp: No