cDNA Clone PKB

• Synonyms: AKT1
• Plasmid: pGEX-PKB 1-149
• Parent Plasmid: pGEX4T1
• Expressed: GST-PH
• Genbank: NP_005154.2
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGAGCGACGTGGCTATTGTGAAGGAGGGTTGGCTGCACAAACG
AGGGGAGTACATCAAGACCTGGCGGCCACGCTACTTCCTCCTCAAGAATG
ATGGCACCTTCATTGGCTACAAGGAGCGGCCGCAGGATGTGGACCAACGT
GAGGCTCCCCTCAACAACTTCTCTGTGGCGCAGTGCCAGCTGATGAAGAC
GGAGCGGCCCCGGCCCAACACCTTCATCATCCGCTGCCTGCAGTGGACCA
CTGTCATCGAACGCACCTTCCATGTGGAGACTCCTGAGGAGCGGGAGGAG
TGGACAACCGCCATCCAGACTGTGGCTGACGGCCTCAAGAAGCAGGAGGA
GGAGGAGATGGACTTCCGGTCGGGCTCACCCAGTGACAACTCAGGGGCTG
AAGAGATGGAGGTGTCCCTGGCCAAGCCCAAGCACCGCGTGACCATGAAC
GAGTGA

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPEFMSDVAIVKEGWLHKRGEYIKT
WRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPN
TFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFR
SGSPSDNSGAEEMEVSLAKPKHRVTMNE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1537
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for bacterial expression