cDNA Clone PKA

• Synonyms: PRKACA, PKA C-alpha, PKACa
• Plasmid: pGEX6P-1-PKA with L274Q
• Parent Plasmid: pGEX6P1
• Expressed: GST-PKA with L274Q
• Genbank: P17612
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGGCAACGCCGCCGCCGCGATGGGCAACGCCGCCGCCGCCAAGAA
GGGCAGCGAGCAGGAGAGCGTGAAAGAATTCTTAGCCAAAGCCAAAGAAG
ATTTTCTTAAAAAATGGGAAAGTCCCGCTCAGAACACAGCCCACTTGGAT
CAGTTTGAACGAATCAAGACCCTCGGCACGGGCTCCTTCGGGCGGGTGAT
GCTGGTGAAACACAAGGAGACCGGGAACCACTATGCCATGAAGATCCTCG
ACAAACAGAAGGTGGTGAAACTGAAACAGATCGAACACACCCTGAATGAA
AAGCGCATCCTGCAAGCTGTCAACTTTCCGTTCCTCGTCAAACTCGAGTT
CTCCTTCAAGGACAACTCAAACTTATACATGGTCATGGAGTACGTGCCCG
GCGGGGAGATGTTCTCACACCTACGGCGGATCGGAAGGTTCAGTGAGCCC
CATGCCCGTTTCTACGCGGCCCAGATCGTCCTGACCTTTGAGTATCTGCA
CTCGCTGGATCTCATCTACAGGGACCTGAAGCCGGAGAATCTGCTCATTG
ACCAGCAGGGCTACATTCAGGTGACAGACTTCGGTTTCGCCAAGCGCGTG
AAGGGCCGCACTTGGACCTTGTGCGGCACCCCTGAGTACCTGGCCCCTGA
GATTATCCTGAGCAAAGGCTACAACAAGGCCGTGGACTGGTGGGCCCTGG
GGGTTCTTATCTATGAAATGGCCGCTGGCTACCCGCCCTTCTTCGCAGAC
CAGCCCATCCAGATCTATGAGAAGATCGTCTCTGGGAAGGTGCGCTTCCC
TTCCCACTTCAGCTCTGACTTGAAGGACCTGCTGCGGAACCTCCAGCAGG
TAGATCTCACCAAGCGCTTTGGGAACCTCAAGAATGGGGTCAACGATATC
AAGAACCACAAGTGGTTTGCCACAACTGACTGGATTGCCATCTACCAGAG
GAAGGTGGAAGCTCCCTTCATACCAAAGTTTAAAGGCCCTGGGGATACGA
GTAACTTTGACGACTATGAGGAAGAAGAAATCCGGGTCTCCATCAATGAG
AAGTGTGGCAAGGAGTTTTCTGAGTTTTAGGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSGNAAAAMGNAAAAKKGSEQ
ESVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKH
KETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKD
NSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDL
IYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILS
KGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFS
SDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEA
PFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU951
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST fusion for bacterial expression. Clone with L274Q corrected = DU53922
• Low Temp: No