cDNA Clone PP1 alpha

• Plasmid: pCW PP1alpha
• Parent Plasmid: pCW
• Expressed: PP1alpha
• Genbank: X14832
• Tag: HA
• Species: Rabbit

Sequence of Insert

ATGTCCGACAGCGAGAAGCTCAACCTGGACTCTATCATCGGGCGCCTGCT
GGAAGTGCAGGGCTCGCGGCCCGGAAAGAATGTGCAGCTGACGGAGAACG
AGATCCGTGGTCTGTGCCTCAAATCCCGGGAGATCTTCCTGAGCCAGCCC
ATTCTGCTGGAGCTGGAGGCGCCCCTCAAGATCTGCGGTGACATCCACGG
CCAGTACTACGACCTGCTGCGGCTGTTCGAGTACGGCGGCTTCCCCCCGG
AGAGCAACTACCTGTTCCTGGGTGACTACGTGGACCGCGGCAAGCAGTCC
CTGGAAACCATCTGCCTGCTGCTGGCCTACAAGATCAAGTACCCCGAGAA
CTTCTTCCTGCTGCGCGGGAACCACGAGTGCGCCAGCATCAACCGCATCT
ACGGCTTCTACGACGAGTGCAAGAGACGCTACAACATCAAGCTGTGGAAG
ACGTTCACCGACTGCTTCAACTGCCTGCCCATCGCGGCCATTGTGGACGA
GAAGATATTCTGCTGCCATGGCGGCCTCTCCCCCGACCTGCAGTCCATGG
AGCAGATCCGGCGCATCATGCGGCCCACGGACGTGCCCGACCAGGGCCTG
CTGTGTGACCTGCTGTGGTCTGACCCCGACAAGGACGTGCAGGGCTGGGG
CGAGAACGACCGCGGCGTCTCCTTCACCTTCGGCGCGGAGGTGGTGGCCA
AGTTCCTGCACAAGCATGACCTGGACCTCATCTGCCGGGCGCACCAGGTG
GTGGAGGACGGCTATGAGTTCTTTGCCAAGCGGCAGCTGGTGACACTTTT
CTCAGCCCCCAACTACTGTGGCGAGTTCGACAACGCCGGAGCCATGATGA
GCGTGGACGAGACCCTCATGTGCTCCTTCCAGATCCTTAAGCCGGCCGAC
AAGAACAAGGGCAAGTACGGGCAGTTCAGTGGCCTGAACCCTGGAGGCCG
ACCCATCACCCCACCCCGCAACTCTGCCAAAGCCAAGAAATAG

Amino Acid Sequence

MSDSEKLNLDSIIGRLLEVQGSRPGKNVQLTENEIRGLCLKSREIFLSQP
ILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQS
LETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWK
TFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGL
LCDLLWSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQV
VEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPAD
KNKGKYGQFSGLNPGGRPITPPRNSAKAKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU938
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for native PP1alpa expression. n.b. Rabbit protein is same sequence as human