cDNA Clone TEV Protease

• Synonyms: -
• Plasmid: pGEX6P1 TEV Protease 6His
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C TEV Protease 6His
• Genbank: NP_734212
• Tag: 6His
• Species: Tobacco Etch Virus

Sequence of Insert

GGATCCGAAAGCTTGTTTAAGGGACCACGTGATTACAACCCGATATCGAG
CACCATTTGTCATTTGACGAATGAATCTGATGGGCACACAACATCGTTGT
ATGGTATTGGATTTGGTCCCTTCATCATTACAAACAAGCACTTGTTTAGA
AGAAATAATGGAACACTGTTGGTCCAATCACTACATGGTGTATTCAAGGT
CAAGAACACCACGACTTTGCAACAACACCTCATTGATGGGAGGGACATGA
TAATTATTCGCATGCCTAAGGATTTCCCACCATTTCCTCAAAAGCTGAAA
TTTAGAGAGCCACAAAGGGAAGAGCGCATATGTCTTGTGACAACCAACTT
CCAAACTAAGAGCATGTCTAGCATGGTGTCAGACACTAGTTGCACATTCC
CTTCATCTGATGGCATATTCTGGAAGCATTGGATTCAAACCAAGGATGGG
CAGTGTGGCAGTCCATTAGTATCAACTAGAGATGGGTTCATTGTTGGTAT
ACACTCAGCATCGAATTTCACCAACACAAACAATTATTTCACAAGCGTGC
CGAAAAACTTCATGGAATTGTTGACAAATCAGGAGGCGCAGCAGTGGGTT
AGTGGTTGGCGATTAAATGCTGACTCAGTATTGTGGGGGGGCCATAAAGT
TTTCATGAGCAAACCTGAAGAGCCTTTTCAGCCAGTTAAGGAAGCGACTC
AACTCATGAATGAATTGGTGTACTCGCAACATCATCACCATCACCATGAA
TTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSESLFKGPRDYNPISSTICH
LTNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLLVQSLHGVFKVKNTT
TLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKS
MSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSAS
NFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMSK
PEEPFQPVKEATQLMNELVYSQHHHHHH

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 TEV Protease 6His306 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU916
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST and C terminal 6His tags
• Low Temp: No