cDNA Clone RSK3

• Plasmid: pGEX6P-1-RSK3
• Parent Plasmid: pGEX6P1
• Expressed: GST-RSK3
• Genbank: CAA59427
• Tag: GST
• Species: Human

Sequence of Insert

atggacctgagcatgaagaagttcgccgtgcgcaggttcttctctgtgta
cctgcgcaggaagtcgcgctccaagagctccagcctgagccggctcgagg
aagaaggtgtcgtgaaggagatagacatcagccatcatgtgaaggagggc
tttgagaaggcagatccttcccagtttgagctgctgaaggttttaggaca
aggatcctatggaaaggtgttcctggtgaggaaggtgaaggggtccgacg
ctgggcagctctacgccatgaaggtccttaagaaagccaccctaaaagtt
cgggaccgagtgagatcgaagatggagagagacatcttggcagaagtgaa
tcaccccttcattgtgaagcttcattatgcctttcagacggaaggaaagc
tctacctgatcctggacttcctgcggggaggggacctcttcacccggctc
tccaaagaggtcatgttcacggaggaggatgtcaagttctacctggctga
gctggccttggctttagaccatctccacagcctggggatcatctacagag
atctgaagcctgagaacatcctcctggatgaagaggggcacattaagatc
acagatttcggcctgagtaaggaggccattgaccacgacaagagagcgta
ctccttctgcgggacgatcgagtacatggcacccgaggtggtgaaccggc
gaggacacacgcagagtgccgactggtggtccttcggcgtgctcatgttt
gagatgctcacggggtccctgccgttccaggggaaggacaggaaggagac
catggctctcatcctcaaagccaagctggggatgccgcagttcctcagtg
gggaggcacagagtttgctgcgagctctcttcaaacggaacccctgcaac
cggctgggtgctggcattgacggagtggaggaaattaagcgccatccctt
ctttgtgaccatagactggaacacgctgtaccggaaggagatcaagccac
cgttcaaaccagcattgggcaggcctgaggacaccttccactttgacccc
gagttcacagcgcggacgcccacagactctcctggcgtccccccgagtgc
aaacgctcatcacctgtttagaggattcagctttgtggcctcaagcctga
tccaggagccctcacagcaagatctgcacaaagtcccagttcacccaatc
gtgcagcagttacacgggaacaacatccacttcaccgatggctacgagat
caaggaggacatcggggtgggctcctactcagtgtgcaagcgatgtgtgc
ataaagccacagacaccgagtatgccgtgaagatcattgataagagcaag
agagacccctcggaagagattgagatcctcctgcggtacggccagcaccc
gaacatcatcaccctcaaggatgtctatgatgatggcaagtttgtgtacc
tggtaatggagctgatgcgtggtggggagctcctggaccgcatcctccgg
cagagatacttctcggagcgcgaagccagtgacgtcctgtgcaccatcac
caagaccatggactacctccattcccagggggttgttcatcgagacctga
agccgagtaacatcctgtacagggatgagtcggggagcccagaatccatc
cgagtctgcgacttcggctttgccaagcagctgcgcgcggggaacgggct
gctcatgacaccctgctacacggccaatttcgtggccccggaggtcctga
agcgtcaaggctatgatgcggcgtgtgacatctggagtttggggatcctg
ttgtacaccatgctggcaggatttaccccttttgcaaatgggccagacga
tacccctgaggagattctggcgcggatcggcagtgggaagtatgcccttt
ctgggggaaactgggactcgatatctgacgcagctaaagacgtcgtgtcc
aagatgctccacgtggaccctcatcagcgcctgacggcgatgcaagtgct
caaacacccgtgggtggtcaacagagagtacctgtccccaaaccagctca
gccgacaggacgtgcacctggtgaagggcgcgatggccgccacctacttt
gctctaaacagaacacctcaggccccgcggctggagcccgtgctgtcatc
caacctggctcagcgcagaggcatgaagagactcacgtccacgcggctgt
ag

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFDLSMKKFAVRRFFSVY
LRRKSRSKSSSLSRLEEEGVVKEIDISHHVKEGFEKADPSQFELLKVLGQ
GSYGKVFLVRKVKGSDAGQLYAMKVLKKATLKVRDRVRSKMERDILAEVN
HPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAE
LALALDHLHSLGIIYRDLKPENILLDEEGHIKITDFGLSKEAIDHDKRAY
SFCGTIEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGSLPFQGKDRKET
MALILKAKLGMPQFLSGEAQSLLRALFKRNPCNRLGAGIDGVEEIKRHPF
FVTIDWNTLYRKEIKPPFKPALGRPEDTFHFDPEFTARTPTDSPGVPPSA
NAHHLFRGFSFVASSLIQEPSQQDLHKVPVHPIVQQLHGNNIHFTDGYEI
KEDIGVGSYSVCKRCVHKATDTEYAVKIIDKSKRDPSEEIEILLRYGQHP
NIITLKDVYDDGKFVYLVMELMRGGELLDRILRQRYFSEREASDVLCTIT
KTMDYLHSQGVVHRDLKPSNILYRDESGSPESIRVCDFGFAKQLRAGNGL
LMTPCYTANFVAPEVLKRQGYDAACDIWSLGILLYTMLAGFTPFANGPDD
TPEEILARIGSGKYALSGGNWDSISDAAKDVVSKMLHVDPHQRLTAMQVL
KHPWVVNREYLSPNQLSRQDVHLVKGAMAATYFALNRTPQAPRLEPVLSS
NLAQRRGMKRLTSTRL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU902
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for Bacterial expression of N terminal GST tag