cDNA Clone TPS5

• Plasmid: pGEX6P-1-TPS5
• Parent Plasmid: pGEX6P1
• Genbank: CAB10557
• Species: Arabidopsis thaliana

Sequence of Insert

DNA sequence of insert GCGGATCCATTAGTGATTATCTTCGACATTGTATCTACTTCACTTGCTGT
AGAGATATGGTATCAAGATCTTATTCAAACCTCTTGGATCTTGCTTCTGG
TAACTTCCATTCGTTTTCTCGAGAAAAGAAGAGGTTTCCAAGAGTAGCAA
CTGTCACTGGTGTCTTATCTGAGCTAGATGATGATAACAACAGCAACAGT
GTCTGCTCTGATGCTCCTTCTTCCGTCACACAAGATCGAATCATCATCGT
TGGAAACCAGCTTCCTATTAAATCACATCGGAACTCTGCTGGTAAATTGA
GTTTTAGTTGGGACAATGACTCGCTTCTCTTGCAGCTTAAAGATGGTATG
CGTGAAGATATGGAAGTTGTCTACATTGGTTGCCTTAAGGAACAGATTGA
TACAGTTGAGCAAGATGATGTTTCTCAAAGGTTGCTTGAGAATTTCAAAT
GTGTTCCTGCTTATATCCCACCTGAGCTATTCACTAAGTACTATCATGGA
TTCTGTAAGCAACATCTATGGCCTTTGTTTCACTACATGCTTCCCTTAAC
TCCTGATCTTGGTGGTAGATTTGATCGTTCTTTATGGCAAGCTTATCTTT
CGGTTAACAAGATCTTTGCTGATAAAGTGATGGAAGTGATTAGTCCTGAT
GATGATTTTGTTTGGGTTCATGACTATCACTTAATGGTTTTGCCTACATT
TCTAAGGAAGAGGTTTAATAGAGTAAAGCTTGGTTTTTTCCTTCATAGCC
CGTTCCCTTCCTCTGAGATTTACCGTACTCTTCCAGTGAGAAATGAGCTC
TTACGTGCACTCCTCAACGCTGATTTGATTGGCTTTCATACCTTTGACTA
TGCAAGACACTTCCTTTCTTGTTGTAGCAGGATGCTTGGTTTATCCTATC
AGTCCAAACGTGGAACCATAGGGCTTGAGTATTATGGTCGAACGGTTAGT
ATCAAGATTCTTCCTGTGGGGATTCATATCAGCCAGCTTCAGTCAATTTT
AAACCTCCCAGAGACTCAGACCAAAGTTGCTGAGCTAAGAGATCAGTTCT
TGGATCAGAAAGTTCTTCTCGGTGTTGATGACATGGACATCTTCAAAGGA
ATCAGCCTCAAACTCTTGGCAATGGAACAACTTCTCACACAGCATCCCGA
GAAGAGAGGTCGAGTTGTACTTGTCCAGATTGCAAACCCTGCAAGAGGTC
GTGGGAAAGACGTTCAGGAGGTTCAGTCTGAAACTGAAGCCACGGTTAAA
AGGATCAATGAAATGTTTGGAAGGCCGGGCTACCAACCCGTGGTTCTGAT
TGATACACCGCTTCAATTCTTTGAGAGGATTGCTTACTATGTCATTGCAG
AGTGTTGTCTTGTTACAGCGGTAAGAGATGGTATGAATCTTATACCTTAT
GAGTACATTATCTGCAGGCAAGGTAATCCGAAACTCAACGAGACTATAGG
CCTTGACCCTTCTGCTGCAAAGAAGAGTATGCTTGTTGTCTCTGAGTTTA
TTGGTTGTTCTCCTTCTTTAAGCGGCGCCATTAGAGTAAATCCGTGGAAC
ATTGATGCTGTGACTGAAGCAATGGACTATGCATTGATAGTTTCAGAAGC
AGAGAAGCAAATGCGTCACGAGAAGCATCACAAATATGTTAGCACACATG
ATGTTGCTTATTGGGCGCGTAGCTTTATACAAGATCTTGAAAGGGCTTGC
GGGGATCATGTGAGGAAGAGGTGTTGGGGGATTGGATTCGGGTTAGGCTT
TAGAGTTGTGGCGCTTGATCCGAGTTTTAAAAAGCTTTCGATTGAGCACA
TTGTCTCAGCTTATAAGAGAACCAAGAACCGAGCCATTTTGTTGGATTAT
GATGGCACAATGGTGCAGCCAGGTTCCATTAGGACAACACCAACCCGCGA
AACAATCGAAATCTTGAACAACCTGTCTAGTGATCCCAAGAATATCGTGT
ACCTCGTCAGTGGGAAAGACAGAAGGACACTAACTGAATGGTTTTCTTCA
TGTGACGATCTCGGTTTGGGTGCAGAGCACGGGTATTTTATAAGGCCAAA
TGATGGAACAGACTGGGAAACGTCGAGTTTGGTATCAGGTTTTGAGTGGA
AACAAATAGCAGAGCCAGTGATGAGACTTTACACTGAGACTACAGATGGA
TCAACAATAGAGACTAAAGAGACTGCTCTCGTTTGGAATTACCAATTCGC
AGATCCTGATTTTGGATCTTGTCAAGCCAAAGAGCTTATGGAACACCTCG
AAAGCGTGCTTACCAATGATCCAGTCTCTGTCAAGACTGGACAACAACTC
GTTGAAGTTAAACCACAGGGTGTGAACAAAGGTCTTGTGGCAGAGAGGCT
TCTAACAACGATGCAAGAAAAAGGGAAACTTTTGGATTTCATTCTCTGCG
TCGGTGATGATCGGTCTGATGAGGATATGTTTGAGGTGATAATGAGTGCT
AAAGATGGTCCAGCTTTGTCTCCTGTGGCTGAGATATTCGCTTGCACCGT
TGGACAAAAGCCAAGCAAAGCAAAATACTATTTAGACGATACTGCGGAGA
TAATCAGAATGCTGGACGGTCTAGCCGCTACCAACACAACTATCTCTGAT
CAAACTGATTCAACCGCCACTGTTCCAACTAAAGATCTGTTTTAAGAATT
CGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSISDYLRHCIYFTCCRDMVS
RSYSNLLDLASGNFHSFSREKKRFPRVATVTGVLSELDDDNNSNSVCSDA
PSSVTQDRIIIVGNQLPIKSHRNSAGKLSFSWDNDSLLLQLKDGMREDME
VVYIGCLKEQIDTVEQDDVSQRLLENFKCVPAYIPPELFTKYYHGFCKQH
LWPLFHYMLPLTPDLGGRFDRSLWQAYLSVNKIFADKVMEVISPDDDFVW
VHDYHLMVLPTFLRKRFNRVKLGFFLHSPFPSSEIYRTLPVRNELLRALL
NADLIGFHTFDYARHFLSCCSRMLGLSYQSKRGTIGLEYYGRTVSIKILP
VGIHISQLQSILNLPETQTKVAELRDQFLDQKVLLGVDDMDIFKGISLKL
LAMEQLLTQHPEKRGRVVLVQIANPARGRGKDVQEVQSETEATVKRINEM
FGRPGYQPVVLIDTPLQFFERIAYYVIAECCLVTAVRDGMNLIPYEYIIC
RQGNPKLNETIGLDPSAAKKSMLVVSEFIGCSPSLSGAIRVNPWNIDAVT
EAMDYALIVSEAEKQMRHEKHHKYVSTHDVAYWARSFIQDLERACGDHVR
KRCWGIGFGLGFRVVALDPSFKKLSIEHIVSAYKRTKNRAILLDYDGTMV
QPGSIRTTPTRETIEILNNLSSDPKNIVYLVSGKDRRTLTEWFSSCDDLG
LGAEHGYFIRPNDGTDWETSSLVSGFEWKQIAEPVMRLYTETTDGSTIET
KETALVWNYQFADPDFGSCQAKELMEHLESVLTNDPVSVKTGQQLVEVKP
QGVNKGLVAERLLTTMQEKGKLLDFILCVGDDRSDEDMFEVIMSAKDGPA
LSPVAEIFACTVGQKPSKAKYYLDDTAEIIRMLDGLAATNTTISDQTDST
ATVPTKDLF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU739
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag.
• Low Temp: No