cDNA Clone PHK muscle

• Plasmid: pGEX6P-1-PBKgamma muscle 1-297
• Parent Plasmid: pGEX6P1
• Expressed: GST-PBKgamma muscle 1-297
• Genbank: X80590
• Tag: GST
• Species: Human

Sequence of Insert

ggatccacccgggacgaggcactgccggactctcattctgcacaggactt
ctatgagaattatgagcccaaagagatcctgggcaggggcgttagcagtg
tggtcaggcgatgcatccacaagcccacgagccaggagtacgccgtgaag
gtcatcgacgtcaccggtggaggcagcttcagcccggaggaggtgcggga
gctgcgagaagccacgctgaaggaggtggacatcctgcgcaaggtctcag
ggcaccccaacatcatacagctgaaggacacttatgagaccaacactttc
ttcttcttggtgtttgacctgatgaagagaggggagctctttgactacct
cactgagaaggtcaccttgagtgagaaggaaaccagaaagatcatgcgag
ctctgctggaggtgatctgcaccttgcacaaactcaacatcgtgcaccgg
gacctgaagcccgagaacattctcttggatgacaacatgaacatcaagct
cacagactttggcttttcctgccagctggagccgggagagaggctgcgag
aggtctgcgggacccccagttacctggcccctgagattatcgagtgctcc
atgaatgaggaccacccgggctacgggaaagaggtggacatgtggagcac
tggcgtcatcatgtacacgctgctggccggctccccgcccttctggcacc
ggaagcagatgctgatgctgaggatgatcatgagcggcaactaccagttt
ggctcgcccgagtgggatgattactcggacaccgtgaaggacctggtctc
ccgattcctggtggtgcaaccccagaaccgctacacagcggaagaggcct
tggcacaccccttcttccagcagtacttggtggaggaagtgcggtaagaa
ttc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSTRDEALPDSHSAQDFYENY
EPKEILGRGVSSVVRRCIHKPTSQEYAVKVIDVTGGGSFSPEEVRELREA
TLKEVDILRKVSGHPNIIQLKDTYETNTFFFLVFDLMKRGELFDYLTEKV
TLSEKETRKIMRALLEVICTLHKLNIVHRDLKPENILLDDNMNIKLTDFG
FSCQLEPGERLREVCGTPSYLAPEIIECSMNEDHPGYGKEVDMWSTGVIM
YTLLAGSPPFWHRKQMLMLRMIMSGNYQFGSPEWDDYSDTVKDLVSRFLV
VQPQNRYTAEEALAHPFFQQYLVEEVR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU733
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag.