cDNA Clone RSK1 (rat)

• Synonyms: MAPKAP-K1a, RPS6KA1
• Plasmid: pMT2 MAPKAPK1a
• Parent Plasmid: PMT2
• Expressed: HA MAPKAPK1a
• Genbank: Q63531
• Tag: HA
• Species: Rat

Sequence of Insert

CTCGAGATGCCGCTCGCCCAGCTCAAGGAACCCTGGCCGCTCATGGAGCT
GGTGCCGCTGGACCCGGAGAATGGACAGGCTTCAGGGGAAGAAGCTGGAC
TTCAGCCATCCAAGGATGAGGGCATCCTCAAGGAGATCTCTATCACACAC
CACGTCAAGGCAGGCTCTGAGAAGGCTGATCCATCCCATTTTGAGCTCCT
CAAGGTTCTGGGCCAAGGATCCTTTGGCAAAGTCTTCCTGGTACGCAAGG
TCACCCGGCCTGACAATGGGCACTTGTATGCCATGAAAGTATTAAAGAAG
GCCACGCTGAAAGTGCGTGACCGTGTTCGGACCAAGATGGAGAGAGACAT
CCTAGCTGACGTGAACCACCCCTTCGTAGTGAAACTGCACTATGCCTTCC
AGACCGAGGGCAAGCTCTATCTTATTCTGGACTTTCTGCGTGGTGGAGAC
CTGTTCACACGACTCTCAAAGGAGGTTATGTTTACAGAGGAGGATGTGAA
GTTTTACCTGGCTGAGCTGGCACTGGGCCTGGACCACCTGCACAGCTTGG
GCATCATTTACAGAGACCTCAAGCCTGAGAATATCCTTTTGGATGAGGAG
GGCCACATCAAACTCACTGACTTTGGCCTGAGCAAGGAGGCCATTGACCA
CGAAAAGAAGGCCTATTCCTTCTGCGGGACCGTGGAGTACATGGCGCCCG
AGGTTGTCAACCGCCAGGGCCACACCCACAGTGCAGATTGGTGGTCCTAT
GGGGTGTTGATGTTTGAGATGCTGACGGGCTCCCTGCCCTTCCAGGGGAA
GGACCGGAAGGAGACCATGACCTTGATTTTGAAGGCAAAGCTAGGCATGC
CCCAGTTTCTGAGCACGGAAGCCCAGAGCCTCCTGCGGGCCCTGTTCAAG
AGGAATCCTGCCAACCGGCTTGGCTCAGGCCCCGATGGGGCTGAGGAAAT
TAAGAGACATATCTTCTACTCTACCATTGACTGGAATAAGCTCTACCGCC
GTGAGATCAAGCCACCTTTCAAGCCCGCTGTGGCCCAGCCGGATGACACC
TTCTACTTTGATACCGAGTTCACGTCACGCACACCCAGGGATTCGCCGGG
CATCCCCCCCAGTGCTGGTGCCCATCAGCTCTTCCGTGGCTTCAGCTTCG
TGGCCACCGGTCTGATGGAGGATGACAGCAAGCCTCGGGCCACCCAGGCT
CCGCTGCACTCGGTGGTACAGCAACTCCACGGGAAGAACTTGGTTTTCAG
CGATGGCTACATAGTAAAGGAGACGATCGGCGTGGGCTCCTACTCTGTGT
GTAAGCGCTGTGTCCACAAGGCCACCAACATGGAGTACGCAGTCAAAGTA
ATCGACAAAAGCAAAAGAGATCCCTCCGAAGAGATCGAGATTCTTCTGCG
GTATGGACAGCACCCCAACATCATCACCCTGAAAGATGTGTATGACGACA
GTAAGCACGTATACCTGGTGACAGAGCTGATGAGGGGCGGGGAGCTGCTG
GATAAGATCCTACGGCAGAAATTCTTCTCAGAGCGGGAGGCCAGCTTCGT
CCTGTACACCATCAGCAAGACTGTGGAATACTTGCACTCCCAAGGGGTCG
TCCACAGGGACCTCAAACCCAGTAACATCCTGTATGTGGATGAGTCTGGG
AACCCCGAATGCCTACGAATATGCGACTTTGGCTTTGCCAAGCAGCTACG
GGCTGAGAACGGGCTTCTCATGACACCTTGCTACACAGCCAACTTTGTGG
CACCTGAGGTGCTGAAGCGTCAGGGCTACGATGAAGGCTGTGACATATGG
AGCCTGGGCGTTCTGCTGTACACGATGCTGGCAGGATACACTCCATTTGC
CAATGGGCCCAGTGATACCCCAGAGGAGATCCTCACCCGGATCAGCAGTG
GGAAGTTCACCCTCAGTGGGGGAAACTGGAACACGGTTTCAGAGACAGCC
AAGGACTTAGTATCTAAGATGCTGCATGTGGACCCCCACCAGCGCCTCAC
AGCCAAACAGGTTCTGCAGCACCCGTGGATCACCCAGAAAGACAAGCTCC
CCCAGAGCCAGTTGTCCCACCAAGACCTGCAGCTTGTGAAGGGGGGCATG
GCAGCTACATATTCTGCACTCAGTAGCTCCAAACCCACCCCCCAGCTCAA
GCCAATCGAGTCGTCCATCCTGGCCCAGCGGCGGGTGAGGAAGCTGCCAT
CCACCACCCTGTGAACGACAGTGCGAGCAAACTCCTCTGAGGCAGAGTCC
TTCCAGAGGGAGCAAGCCTGAGTCACAGACCAAGTGGAATGGAGTCCTAA
AGGAAGCAACTAGCCCAGCTCACCCGTGCGGGTGTGAAGTGCCTTCCTCC
CCAGGACGGGCTCTTCTGGGCTCAGGCTCCATTGTGTGAAATCCACTCAC
TGTACAAACTATTTTTAAGAAAGGAAAAAGAAAAAATGACATCATTTACC
ATGGATTTTTTTTTTACAAGATCCATTTGGCTTTTTGGCCATTGCAGTCC
CAGGAGGAACACCCAGTCCCATGTGTGGCCAAGACTCCCGTGATAGCTTT
GGGACTCCGCCCCTCTGTTGGTCAAGGAGCCATCTGCACCCGCCTCCGAG
CACGTTCGGCGTTGCCTCTCAGAGTTGTCGAC

Amino Acid Sequence

MYPYDVPDYALEMPLAQLKEPWPLMELVPLDPENGQASGEEAGLQPSKDE
GILKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDNG
HLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLY
LILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDL
KPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQG
HTHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTE
AQSLLRALFKRNPANRLGSGPDGAEEIKRHIFYSTIDWNKLYRREIKPPF
KPAVAQPDDTFYFDTEFTSRTPRDSPGIPPSAGAHQLFRGFSFVATGLME
DDSKPRATQAPLHSVVQQLHGKNLVFSDGYIVKETIGVGSYSVCKRCVHK
ATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDSKHVYLV
TELMRGGELLDKILRQKFFSEREASFVLYTISKTVEYLHSQGVVHRDLKP
SNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKR
QGYDEGCDIWSLGVLLYTMLAGYTPFANGPSDTPEEILTRISSGKFTLSG
GNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWITQKDKLPQSQLSH
QDLQLVKGGMAATYSALSSSKPTPQLKPIESSILAQRRVRKLPSTTL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU591
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian cell expression. Insert contains 3’ UTR sequence.