cDNA Clone SGK1

• Plasmid: pCMV5 HA SGK1 K127A
• Parent Plasmid: pCMV5
• Expressed: HA SGK1 K127A
• Genbank: AAH01263
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCAGATCCGCCACCATGTATCCATATGATGTTCCAGATTATGCTAC
GGTGAAAACTGAGGCTGCTAAGGGCACCCTCACTTACTCCAGGATGAGGG
GCATGGTGGCAATTCTCATCGCTTTCATGAAGCAGAGGAGGATGGGTCTG
AACGACTTTATTCAGAAGATTGCCAATAACTCCTATGCATGCAAACACCC
TGAAGTTCAGTCCATCTTGAAGATCTCCCAACCTCAGGAGCCTGAGCTTA
TGAATGCCAACCCTTCTCCTCCACCAAGTCCTTCTCAGCAAATCAACCTT
GGCCCGTCGTCCAATCCTCATGCTAAACCATCTGACTTTCACTTCTTGAA
AGTGATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTAGCAAGACACAAGG
CAGAAGAAGTGTTCTATGCAGTCGCAGTTTTACAGAAGAAAGCAATCCTG
AAAAAGAAAGAGGAGAAGCATATTATGTCGGAGCGGAATGTTCTGTTGAA
GAATGTGAAGCACCCTTTCCTGGTGGGCCTTCACTTCTCTTTCCAGACTG
CTGACAAATTGTACTTTGTCCTAGACTACATTAATGGTGGAGAGTTGTTC
TACCATCTCCAGAGGGAACGCTGCTTCCTGGAACCACGGGCTCGTTTCTA
TGCTGCTGAAATAGCCAGTGCCTTGGGCTACCTGCATTCACTGAACATCG
TTTATAGAGACTTAAAACCAGAGAATATTTTGCTAGATTCACAGGGACAC
ATTGTCCTTACTGATTTCGGACTCTGCAAGGAGAACATTGAACACAACAG
CACAACATCCACCTTCTGTGGCACGCCGGAGTATCTCGCACCTGAGGTGC
TTCATAAGCAGCCTTATGACAGGACTGTGGACTGGTGGTGCCTGGGAGCT
GTCTTGTATGAGATGCTGTATGGCCTGCCGCCTTTTTATAGCCGAAACAC
AGCTGAAATGTACGACAACATTCTGAACAAGCCTCTCCAGCTGAAACCAA
ATATTACAAATTCCGCAAGACACCTCCTGGAGGGCCTCCTGCAGAAGGAC
AGGACAAAGCGGCTCGGGGCCAAGGATGACTTCATGGAGATTAAGAGTCA
TGTCTTCTTCTCCTTAATTAACTGGGATGATCTCATTAATAAGAAGATTA
CTCCCCCTTTTAACCCAAATGTGAGTGGGCCCAACGACCTACGGCACTTT
GACCCCGAGTTTACCGAAGAGCCTGTCCCCAACTCCATTGGCAAGTCCCC
TGACAGCGTCCTCGTCACAGCCAGCGTCAAGGAAGCTGCCGAGGCTTTCC
TAGGCTTTTCCTATGCGCCTCCCACGGACTCTTTCCTCTGAGTCGAC

Amino Acid Sequence

MYPYDVPDYATVKTEAAKGTLTYSRMRGMVAILIAFMKQRRMGLNDFIQK
IANNSYACKHPEVQSILKISQPQEPELMNANPSPPPSPSQQINLGPSSNP
HAKPSDFHFLKVIGKGSFGKVLLARHKAEEVFYAVAVLQKKAILKKKEEK
HIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRE
RCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDF
GLCKENIEHNSTTSTFCGTPEYLAPEVLHKQPYDRTVDWWCLGAVLYEML
YGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDRTKRLG
AKDDFMEIKSHVFFSLINWDDLINKKITPPFNPNVSGPNDLRHFDPEFTE
EPVPNSIGKSPDSVLVTASVKEAAEAFLGFSYAPPTDSFL*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5 HA SGK1 K127A56.21 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU154
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression. Kinase dead mutant.
• Low Temp: No