cDNA Clone PPP1R10 (PNUTS)

• Plasmid: pET43 DU 6xHis PPP1R10
• Parent Plasmid: pGEX6P1
• Expressed: NUS-6xHis-S.TAG-Prec-6xHis-PPP1R10
• Genbank: NM_002714.2
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGGTTCGGGTCCCATAGACCCCAAAGAACTTCTCAAGGGCCTGGACAGCTTCCTTAACCGAGATGGGGAAGTCAAAAGTGTGGATGGGATTTCCAAGATCTTCAGTTTGATGAAGGAAGCACGAAAGATGGTGAGTCGATGCACTTACTTGAACATTCTCCTGCAGACCCGTTCACCAGAAATATTGGTCAAATTTATTGACGTTGGCGGCTACAAACTTCTTAACAATTGGCTGACGTATTCAAAGACAACCAACAACATTCCCCTCCTCCAGCAAATTCTACTGACCCTGCAGCATCTACCGCTCACTGTAGACCATCTCAAGCAGAACAACACAGCTAAACTGGTGAAGCAGCTGAGCAAGTCAAGTGAGGATGAAGAGCTCCGGAAATTGGCCTCAGTCCTTGTCAGCGACTGGATGGCTGTCATCCGCTCTCAGAGCAGTACCCAGCCTGCTGAGAAAGATAAGAAGAAACGTAAAGATGAAGGAAAAAGTCGAACTACCCTTCCTGAGCGACCTTTGACAGAGGTGAAGGCTGAGACCCGGGCTGAGGAGGCCCCAGAGAAGAAGAGGGAGAAGCCCAAGTCTCTTCGCACCACAGCACCCAGTCATGCCAAGTTCCGTTCCACTGGACTAGAGCTGGAGACACCATCCTTGGTGCCTGTGAAGAAGAATGCCAGCACAGTGGTGGTTTCTGACAAGTACAACCTTAAACCCATCCCCCTCAAACGTCAGAGCAACGTAGCTGCTCCAGGAGATGCCACTCCCCCTGCAGAGAAGAAATACAAGCCACTCAACACAACACCTAATGCCACCAAAGAGATCAAAGTGAAGATCATCCCGCCACAGCCTATGGAGGGCCTGGGCTTTCTGGATGCTCTTAATTCAGCCCCTGTTCCAGGCATCAAAATTAAGAAGAAAAAAAAAGTACTGTCACCTACGGCTGCCAAGCCAAGCCCCTTTGAAGGGAAAACGAGCACAGAACCAAGCACAGCCAAACCTTCTTCCCCAGAACCAGCACCACCTTCTGAGGCAATGGACGCAGACCGTCCAGGCACCCCGGTTCCCCCTGTTGAAGTCCCGGAGCTCATGGATACAGCCTCTTTGGAGCCAGGAGCTCTGGATGCCAAGCCAGTGGAGAGTCCTGGAGATCCTAACCAACTGACCCGGAAAGGCAGGAAGAGGAAAAGTGTGACATGGCCTGAGGAAGGCAAACTGAGAGAATATTTCTATTTTGAATTGGATGAAACTGAACGAGTAAATGTGAATAAGATCAAGGACTTTGGTGAGGCGGCTAAGCGAGAGATACTGTCAGACCGACATGCATTTGAGACAGCGCGGCGTCTGAGCCATGATAACATGGAGGAGAAGGTGCCCTGGGTGTGCCCCCGGCCCCTGGTTCTGCCCTCACCTCTTGTCACCCCTGGAAGCAATAGTCAGGAGCGATATATCCAGGCTGAGCGGGAGAAGGGAATCCTTCAGGAGCTCTTCCTGAACAAGGAGAGTCCTCATGAGCCTGATCCTGAGCCCTACGAGCCCATACCCCCTAAACTCATCCCCCTAGATGAGGAGTGTTCCATGGATGAGACTCCGTATGTTGAGACTCTGGAACCTGGGGGGTCAGGTGGCTCACCTGATGGGGCAGGAGGCTCCAAGTTGCCTCCAGTTCTGGCCAATCTTATGGGAAGCATGGGTGCTGGAAAGGGCCCCCAAGGCCCTGGAGGAGGAGGCATTAATGTCCAAGAGATCCTCACCTCCATCATGGGTAGCCCAAACAGTCATCCTTCAGAGGAACTACTGAAACAACCAGACTATTCGGACAAGATCAAGCAGATGCTGGTGCCACATGGACTCCTAGGCCCTGGCCCAATAGCCAATGGTTTCCCACCAGGGGGTCCTGGGGGCCCCAAGGGCATGCAGCACTTTCCCCCTGGACCTGGGGGACCTATGCCAGGTCCCCATGGAGGCCCTGGTGGGCCAGTGGGTCCACGTCTTCTGGGTCCTCCACCCCCTCCCCGGGGAGGTGATCCCTTCTGGGATGGCCCGGGCGACCCTATGCGGGGTGGCCCAATGCGGGGGGGTCCAGGACCAGGTCCTGGACCATACCATAGAGGCCGAGGTGGCCGAGGAGGAAACGAACCTCCTCCTCCTCCTCCTCCATTCCGAGGCGCCAGAGGAGGTCGCTCTGGAGGAGGACCCCCAAATGGACGAGGGGGCCCTGGTGGGGGCATGGTTGGAGGTGGTGGGCATCGTCCTCACGAAGGCCCTGGTGGGGGCATGGGCAACAGCAGTGGACATCGTCCCCACGAAGGCCCTGGCGGTGGCATGGGAAGTGGGCATCGCCCCCATGAAGGCCCTGGTGGTAGCATGGGTGGGGGTGGAGGACATCGTCCCCACGAAGGCCCTGGCGGTGGCATCAGTGGTGGCAGTGGCCATCGTCCCCATGAAGGCCCTGGCGGAGGAATGGGTGCCGGTGGTGGACATCGCCCCCACGAAGGCCCTGGCGGAAGCATGGGTGGAAGTGGTGGACATCGTCCCCATGAAGGCCCTGGACACGGGGGGCCCCATGGCCACCGGCCTCATGATGTCCCTGGTCACCGAGGCCATGACCATCGAGGGCCGCCACCTCATGAGCACCGTGGCCATGATGGTCCTGGCCACGGGGGAGGGGGCCACCGAGGGCACGATGGAGGCCACAGCCATGGAGGAGACATGTCAAACCGCCCTGTCTGCCGACATTTCATGATGAAGGGCAACTGCCGCTATGAGAACAACTGTGCCTTCTACCACCCGGGTGTCAATGGGCCCCCCCTGCCCTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGSGPIDPKELLKGLDSFLNRDGEVKSVDGISKIFSLMKEARKMVSRCTYLNILLQTRSPEILVKFIDVGGYKLLNNWLTYSKTTNNIPLLQQILLTLQHLPLTVDHLKQNNTAKLVKQLSKSSEDEELRKLASVLVSDWMAVIRSQSSTQPAEKDKKKRKDEGKSRTTLPERPLTEVKAETRAEEAPEKKREKPKSLRTTAPSHAKFRSTGLELETPSLVPVKKNASTVVVSDKYNLKPIPLKRQSNVAAPGDATPPAEKKYKPLNTTPNATKEIKVKIIPPQPMEGLGFLDALNSAPVPGIKIKKKKKVLSPTAAKPSPFEGKTSTEPSTAKPSSPEPAPPSEAMDADRPGTPVPPVEVPELMDTASLEPGALDAKPVESPGDPNQLTRKGRKRKSVTWPEEGKLREYFYFELDETERVNVNKIKDFGEAAKREILSDRHAFETARRLSHDNMEEKVPWVCPRPLVLPSPLVTPGSNSQERYIQAEREKGILQELFLNKESPHEPDPEPYEPIPPKLIPLDEECSMDETPYVETLEPGGSGGSPDGAGGSKLPPVLANLMGSMGAGKGPQGPGGGGINVQEILTSIMGSPNSHPSEELLKQPDYSDKIKQMLVPHGLLGPGPIANGFPPGGPGGPKGMQHFPPGPGGPMPGPHGGPGGPVGPRLLGPPPPPRGGDPFWDGPGDPMRGGPMRGGPGPGPGPYHRGRGGRGGNEPPPPPPPFRGARGGRSGGGPPNGRGGPGGGMVGGGGHRPHEGPGGGMGNSSGHRPHEGPGGGMGSGHRPHEGPGGSMGGGGGHRPHEGPGGGISGGSGHRPHEGPGGGMGAGGGHRPHEGPGGSMGGSGGHRPHEGPGHGGPHGHRPHDVPGHRGHDHRGPPPHEHRGHDGPGHGGGGHRGHDGGHSHGGDMSNRPVCRHFMMKGNCRYENNCAFYHPGVNGPPLP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU37545
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 125882.24 Daltons