cDNA Clone SLX4 (mouse)

• Synonyms: AI256635, AI426760, Btbd12, D16Bwg1016e
• Plasmid: pBabe.puro-DU SLX4m
• Parent Plasmid: pBabeD puro
• Expressed: SLX4m
• Genbank: NM_177472.4
• Species: Mouse

Sequence of Insert

GAATTCATGGTTCCAGAGAGTGCTCCCAATGGCAACTCCCAGCCTCTTCCTTCCTGTTTCACGACAACTGGTGTACCAAGTCCCTCCAAACCCAGAGTCTCAGAGCTGGTTCTGCAACGGATGAAGCAGTTCAAGAGAGCAGACCCTGAGCGCCTGAGACACGCCTCAGAAGAATCCCCCCAAAAGACTGCACTGGGAGACGATGTCCCAAGGAGCCCTCCAGAGGAGACAGTGGGAGAAAATGAGTATAAACTTGATGCCACAGACAGTGATGCTGCAATGGCCCTGGCCTTACAGCAGGAGTTTAGAAGGGAGGAAGCATCCTCACACCATGACAGCCTGGAGGAGAAGGGGTTGTTCTTCTGTCAAATGTGTCAGAAAAACCTCTCAGCAATGAATGTAACCCGGAGGGAGCAACATGTGAACAGATGCCTAGATGAGGCAGAGAAGGCACAGAGACCTGCTTCACCTCGGATCCCTGATTGCCCAATATGTGGGAAACCGTTCCTCACCACCAAGAGCCGAATCAGTCACTTGAAGCAGTGTGCGGTGAGGATGGAAGTGGGTCCCCAACTCCTGCTTCAGGCTGTGAGGCTACAGACAGCTCAGCCAGAGGTGGATGGCAGCCCTCAGGTACCCAGTTTCAGCAACAACGTTGGAGGTTTGAAACGGAAAGGAGTCACCACCAAAAGGGAGCCAAGGAGGAGGAAAGTCAATAAGCCTGAGGCTCCGTCTGAAGATCTGTTGGTGGCCATGGCTCTGTCTCGCTCTGAGGTGGAGCACTGTCCAGTTGTGCCCCCGCTCAGACTGGAAAATGCTTTTTCTGAGAAGATAAGGCTAGGAGCAGAGAAGAAAAGTCGCAAGAAGAGACCCCCGGTGTGTCCCCCACAGTTGGTAACCCAAGACTCTGAGACCACAGGTAGACAGATTGAAGATCGTGTTGCCCAGCTTCTGTCAGAGGAAGCAGAACTATCTTGCACCCCACCACTTCTTGCCAGCAAGATTTCTAAGGAAGAATTGGAACCTGCAGGCTGGCGAGCACGGCTGCCTGAAGGGAAGCGGAACTTTCTGTGGGAACTCAGCGCTCTCACAGGAGCCTGGGCTGAGGAGTCCTTCTACACAGTGGGACTGTTTCCTCCAATTGTGTCCCAATGCCCTTCCAAGGAACCCCAGCTACCATTGGAGCTGCCCAAGCAAGGAGAGCCGAGTCCACGGCGGCCACCTGCTTCCCAGAGCAGCCTTCCTGTGAGCCACAGCCCCAAGATCCGGCTGCTGTCCTCCAGCCAGAGGGAGCGTCAAGCCCTTCAGGACCTTGTGGACCTGGCAGTGGAAGGGCTGAGCTCTAGCCCACAGCCCGGGAGTCGGGGCGTACCCACAGGGTTGGACCTGGTACCTAGCAGCCTTCCGCTGACGGGGTTTGTCCTTCCATGTAAGAAGACCCTGAAGAAGGATGACAGTGCCTCACTCTCTCTTGGGTTATTGGTGACAGACTTTGGGGCCATGGTCAATAATCCACACCTGAGTGATGTCCAGTTCCAGCTTGACAGTGGAGAGGTGCTTTATGCACACAAGTTTGTTCTTTATGCGAGATGCCCACTTCTTATTCAGTATGTAAGCACTGAAAGCTTTTCTTCTGAAGAGGACGGGGATCTGACCCAGCGTGCTCTGCTGAGTGATGTGAGCTCGGAGGCCGCCCATGCATTTCTGAACTACCTCTACATGGCAGACACTGACATGCCTCCCAGCCTGGTTCCGGATCTGCGTTCCCTGGCCCTAAGGTTTGGTGTGAGTGACCTTGTTCAGCTGTGTGAACAAGTGCCCGCCGTGGTGGACTTAGAGGGAGAACAGCCAGAAGAGACCAGTGAGGACTGTGAAAGCCGAGCAGAGACTTTCCTGGAACTCCTGAGGTCGGTGTGGGTAGACAATGAGGAGGAAGTGGAGACTCTGCTGAAACCTGAGCTCTGTGAAGAAGAGAGAGAAAGAGTGAATGAAGCAGAAATGGAAGAGATATATGAATTTGCTGCTACGCAGAGGAAGCTTCTCCAGTGGGGGAGAGCAGCAGACCCAGATGGGAGCACTAACCCGCATGGGGAAGACGGTGCAGTTTCTGAGCCTTCCCTGGCAGGTGTCCAGAGTAACAGACAGCTAGAAAATACAGAGCACATGGAGTCATCTGGGTTAGAAAAAGAAGAGGCACTAGCCAGCTGGGAACAAGAAGGACATTCCACTCCACTGCAGGACCAGTGCCCAGACTGGGCAGGGAAAGCAGAGGCCCAGGATGCATTGGGGGAGGCAACTGACGACCCCAGCTTCTGCAGCCGCCACAGGAGGGGGAAAGAGTGCTTGCCTTTGCACCCAAACAAGGCCCATGGCTGCAAACAGCCCCTTCCATCAAATCCAAGAGTGTCATCTGAACTGTCACAAATAACAGTTGATCATGAAGAGCAGAGTGACCATGTCAGGGAGACGCAGGCAGACATGGCCCAGGCACCTACTCCACACTCCTGCAGCCTTGTGTCACAGTCTTCTGTGGATGGAAGTCCCAGCCAGTCATGGCTTCACCTATACCACACTAGTCATTTGTCCCCCTCAGTGTCCCAGTCACACAGTAGCATTTCCAGGGTGGCATCCCCAAGATCACTGTCTCCAACTACACCAACTAAGCAGAGGAGGGGCAGTAACATTGTCACATTACGGAAAGATGCTGGCCACCACAGAGGCCAACAAAGTAGCCCCATAGCGGGACACAGAAATAGGGGCATCCTGATTTCCCCAGCAAAGTCTCCACCCATTGACCTCACACAGTCAGTACCCGAGCCCCTGAGCCCCAGGGCCCAGGATCCTCTGCATTTCGTGAAAAAGGAGGATGAGGTCATCCTGTTACTGGACTCAGATGAGGAGCTGGAGCACACCAAGACAGAGTCAGTTTCTAAAGATTCCCCAGAAGGAAGGAAAGTTCCAGAATTTAGCCCCAGGTCCTCTGAACTGTTTTCAGTCATTGATGTTGAAGAAGATCATGAACATTTCCAAAGCCCACTGAAGAGAGAGGCCGGGCTACAGCACGGGGAGGAAGGACAGCTGGGAAACCAGTCTGCGCTGGGCTGCAGAGACATCCCCTGGCTGCTCTGCAGCCAGAAAACCAGCCTGGATGAGGACAGTGCTACAGACACCTCTTGGCTGGTGCCTGCCACTCCAGGGGTCAGCAGGAGTCGTGATTGCTCATCACAGACCCAAATCAAAAGCCTTAAGACCAGGATTCCGTCAGATGAGACTGCTCAGCAAACACCTAGACCTAACTTAGAACGCAGGACCATGCTGGAAACAGCCCAGCAGTTCTCTGTGATCATGCCACACACACAGCCTATTACTCTAGGAGCCTTTGACAGCGGAAGACAGGCCTACAGAAGCCCTTCCCATCCCTACCCCAGGCACCACAGACTCTCTTCTTCACAGCCATCATGTCCTGGCCCTGATTTTACCAGGTGGTCCCAGAAATCTTCCGCACCTAGACCATGCCTGCCAAATCTGCCTGCAGCTGATGATGTGGTAGAAGTTGGGGACAGTGATGATGAGGTGGCTTCCCATCAAGGAAACAGCAGCCCCGTGCTAGATGGTGACCCCCCAGGTCCCATGGGTGACTACTGTTGGAATGAGCCTCTCTCACCAATTCCAATTGACCACTTGAATCTGGAGCGGACCGGCCCCCTGACCACAAGCAGTCCCAGCAGTCAGGTCCTGGAGGCTTTGCACAGTGATGACTGCCACTCCCCGGGACTTGGTACCACCCCCATCCGAGGAAGCTGTGGTACGCTGAGAGAGTCTCAGGAACGGTCCTCACTGGCTGGCTCTCCAGAGGCTCTGTGGGACGACTGGAATGAGGAGGAAGGACAGTCTCCAGAGGCCCCACCTGTGGCTCAAATGCTGAGTACCAGAACTCGGAAGCCAGACAGGCCAGAGACACCAAAAGGTGCTAACCAGAAAAAGAACTTGCCTCCCAAAGTGCCTATAACTCCAATGCCAAGGTATTCGATCATGGAGACCCCGGTGCTGAAGAAAGAACTGGACAGGTTTGGAGTCCGTGCTCTGCCCAAACGCCAGATGGTTCTAAAACTGAAGGAGATTTTCCAGTACACTCACCAGACTCTGGAGTCGGACTCTGAGGATGAGGTCCAGTCCCCCCAGATACCCGCAGAACTACCTTGTCGCCAGGCATCTACCACTGAGACCTGCAACCCTTCAAGGTTGCCAACTGGGGAGCCTTCACATCCTGATGGTGACGCCCAGCTCCCAGCCTCCCAGGAATCCATGGCTACCTCTGTGGACGGCAGCGACAACTCCTTTAGCTCTAAAAGCTCCTCTGCTGAGTTTGGAGCTGCGTTTGAGTATTCAGATGAAGACAAGGATGAGGAGGTCGGGGTCACTGCATCACAGGCCGCAATCCAGGCCGCAGACACAGAGGAGGCAGTGAGGCGCTATATCCGCTCTAAGCCTGCCCTGCACAGGCAGGTCCTGAGGTACCAGCCTGTGGAGCTGGCCGAGCTGCAGGCTGAGCTGAAACAGAATGGCATCCCTGTGGCCATGGGCAAGCTGTCGGACATCCTGGACGCTCAGTGCATCACCTTCACCACTGCTGCAGCCCGAAAGGAGAAACTCAAACACAAGAGACGGCAGCCCTCAGGCCGAAAGAAAAAGGATCAGAAATGAGCGGCCGC

Amino Acid Sequence

MVPESAPNGNSQPLPSCFTTTGVPSPSKPRVSELVLQRMKQFKRADPERLRHASEESPQKTALGDDVPRSPPEETVGENEYKLDATDSDAAMALALQQEFRREEASSHHDSLEEKGLFFCQMCQKNLSAMNVTRREQHVNRCLDEAEKAQRPASPRIPDCPICGKPFLTTKSRISHLKQCAVRMEVGPQLLLQAVRLQTAQPEVDGSPQVPSFSNNVGGLKRKGVTTKREPRRRKVNKPEAPSEDLLVAMALSRSEVEHCPVVPPLRLENAFSEKIRLGAEKKSRKKRPPVCPPQLVTQDSETTGRQIEDRVAQLLSEEAELSCTPPLLASKISKEELEPAGWRARLPEGKRNFLWELSALTGAWAEESFYTVGLFPPIVSQCPSKEPQLPLELPKQGEPSPRRPPASQSSLPVSHSPKIRLLSSSQRERQALQDLVDLAVEGLSSSPQPGSRGVPTGLDLVPSSLPLTGFVLPCKKTLKKDDSASLSLGLLVTDFGAMVNNPHLSDVQFQLDSGEVLYAHKFVLYARCPLLIQYVSTESFSSEEDGDLTQRALLSDVSSEAAHAFLNYLYMADTDMPPSLVPDLRSLALRFGVSDLVQLCEQVPAVVDLEGEQPEETSEDCESRAETFLELLRSVWVDNEEEVETLLKPELCEEERERVNEAEMEEIYEFAATQRKLLQWGRAADPDGSTNPHGEDGAVSEPSLAGVQSNRQLENTEHMESSGLEKEEALASWEQEGHSTPLQDQCPDWAGKAEAQDALGEATDDPSFCSRHRRGKECLPLHPNKAHGCKQPLPSNPRVSSELSQITVDHEEQSDHVRETQADMAQAPTPHSCSLVSQSSVDGSPSQSWLHLYHTSHLSPSVSQSHSSISRVASPRSLSPTTPTKQRRGSNIVTLRKDAGHHRGQQSSPIAGHRNRGILISPAKSPPIDLTQSVPEPLSPRAQDPLHFVKKEDEVILLLDSDEELEHTKTESVSKDSPEGRKVPEFSPRSSELFSVIDVEEDHEHFQSPLKREAGLQHGEEGQLGNQSALGCRDIPWLLCSQKTSLDEDSATDTSWLVPATPGVSRSRDCSSQTQIKSLKTRIPSDETAQQTPRPNLERRTMLETAQQFSVIMPHTQPITLGAFDSGRQAYRSPSHPYPRHHRLSSSQPSCPGPDFTRWSQKSSAPRPCLPNLPAADDVVEVGDSDDEVASHQGNSSPVLDGDPPGPMGDYCWNEPLSPIPIDHLNLERTGPLTTSSPSSQVLEALHSDDCHSPGLGTTPIRGSCGTLRESQERSSLAGSPEALWDDWNEEEGQSPEAPPVAQMLSTRTRKPDRPETPKGANQKKNLPPKVPITPMPRYSIMETPVLKKELDRFGVRALPKRQMVLKLKEIFQYTHQTLESDSEDEVQSPQIPAELPCRQASTTETCNPSRLPTGEPSHPDGDAQLPASQESMATSVDGSDNSFSSKSSSAEFGAAFEYSDEDKDEEVGVTASQAAIQAADTEEAVRRYIRSKPALHRQVLRYQPVELAELQAELKQNGIPVAMGKLSDILDAQCITFTTAAARKEKLKHKRRQPSGRKKKDQK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU33993
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent changes T>C 1315 and 4323 bp.; ; All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.