cDNA Clone SLX4 (mouse)

• Plasmid: pFB HTA mSLX4
• Parent Plasmid: pSC-A
• Expressed: 6xHis-mSLX4
• Genbank: NM_177472.4
• Tag: 6His
• Species: Mouse

Sequence of Insert

GAATTCATGGTTCCAGAGAGTGCTCCCAATGGCAACTCCCAGCCTCTTCCTTCCTGTTTCACGACAACTGGTGTACCAAGTCCCTCCAAACCCAGAGTCTCAGAGCTGGTTCTGCAACGGATGAAGCAGTTCAAGAGAGCAGACCCTGAGCGCCTGAGACACGCCTCAGAAGAATCCCCCCAAAAGACTGCACTGGGAGACGATGTCCCAAGGAGCCCTCCAGAGGAGACAGTGGGAGAAAATGAGTATAAACTTGATGCCACAGACAGTGATGCTGCAATGGCCCTGGCCTTACAGCAGGAGTTTAGAAGGGAGGAAGCATCCTCACACCATGACAGCCTGGAGGAGAAGGGGTTGTTCTTCTGTCAAATGTGTCAGAAAAACCTCTCAGCAATGAATGTAACCCGGAGGGAGCAACATGTGAACAGATGCCTAGATGAGGCAGAGAAGGCACAGAGACCTGCTTCACCTCGGATCCCTGATTGCCCAATATGTGGGAAACCGTTCCTCACCACCAAGAGCCGAATCAGTCACTTGAAGCAGTGTGCGGTGAGGATGGAAGTGGGTCCCCAACTCCTGCTTCAGGCTGTGAGGCTACAGACAGCTCAGCCAGAGGTGGATGGCAGCCCTCAGGTACCCAGTTTCAGCAACAACGTTGGAGGTTTGAAACGGAAAGGAGTCACCACCAAAAGGGAGCCAAGGAGGAGGAAAGTCAATAAGCCTGAGGCTCCGTCTGAAGATCTGTTGGTGGCCATGGCTCTGTCTCGCTCTGAGGTGGAGCACTGTCCAGTTGTGCCCCCGCTCAGACTGGAAAATGCTTTTTCTGAGAAGATAAGGCTAGGAGCAGAGAAGAAAAGTCGCAAGAAGAGACCCCCGGTGTGTCCCCCACAGTTGGTAACCCAAGACTCTGAGACCACAGGTAGACAGATTGAAGATCGTGTTGCCCAGCTTCTGTCAGAGGAAGCAGAACTATCTTGCACCCCACCACTTCTTGCCAGCAAGATTTCTAAGGAAGAATTGGAACCTGCAGGCTGGCGAGCACGGCTGCCTGAAGGGAAGCGGAACTTTCTGTGGGAACTCAGCGCTCTCACAGGAGCCTGGGCTGAGGAGTCCTTCTACACAGTGGGACTGTTTCCTCCAATTGTGTCCCAATGCCCTTCCAAGGAACCCCAGCTACCATTGGAGCTGCCCAAGCAAGGAGAGCCGAGTCCACGGCGGCCACCTGCTTCCCAGAGCAGCCTTCCTGTGAGCCACAGCCCCAAGATCCGGCTGCTGTCCTCCAGCCAGAGGGAGCGTCAAGCCCTTCAGGACCTTGTGGACCTGGCAGTGGAAGGGCTGAGCTCTAGCCCACAGCCCGGGAGTCGGGGCGTACCCACAGGGTTGGACCTGGTACCTAGCAGCCTTCCGCTGACGGGGTTTGTCCTTCCATGTAAGAAGACCCTGAAGAAGGATGACAGTGCCTCACTCTCTCTTGGGTTATTGGTGACAGACTTTGGGGCCATGGTCAATAATCCACACCTGAGTGATGTCCAGTTCCAGCTTGACAGTGGAGAGGTGCTTTATGCACACAAGTTTGTTCTTTATGCGAGATGCCCACTTCTTATTCAGTATGTAAGCACTGAAAGCTTTTCTTCTGAAGAGGACGGGGATCTGACCCAGCGTGCTCTGCTGAGTGATGTGAGCTCGGAGGCCGCCCATGCATTTCTGAACTACCTCTACATGGCAGACACTGACATGCCTCCCAGCCTGGTTCCGGATCTGCGTTCCCTGGCCCTAAGGTTTGGTGTGAGTGACCTTGTTCAGCTGTGTGAACAAGTGCCCGCCGTGGTGGACTTAGAGGGAGAACAGCCAGAAGAGACCAGTGAGGACTGTGAAAGCCGAGCAGAGACTTTCCTGGAACTCCTGAGGTCGGTGTGGGTAGACAATGAGGAGGAAGTGGAGACTCTGCTGAAACCTGAGCTCTGTGAAGAAGAGAGAGAAAGAGTGAATGAAGCAGAAATGGAAGAGATATATGAATTTGCTGCTACGCAGAGGAAGCTTCTCCAGTGGGGGAGAGCAGCAGACCCAGATGGGAGCACTAACCCGCATGGGGAAGACGGTGCAGTTTCTGAGCCTTCCCTGGCAGGTGTCCAGAGTAACAGACAGCTAGAAAATACAGAGCACATGGAGTCATCTGGGTTAGAAAAAGAAGAGGCACTAGCCAGCTGGGAACAAGAAGGACATTCCACTCCACTGCAGGACCAGTGCCCAGACTGGGCAGGGAAAGCAGAGGCCCAGGATGCATTGGGGGAGGCAACTGACGACCCCAGCTTCTGCAGCCGCCACAGGAGGGGGAAAGAGTGCTTGCCTTTGCACCCAAACAAGGCCCATGGCTGCAAACAGCCCCTTCCATCAAATCCAAGAGTGTCATCTGAACTGTCACAAATAACAGTTGATCATGAAGAGCAGAGTGACCATGTCAGGGAGACGCAGGCAGACATGGCCCAGGCACCTACTCCACACTCCTGCAGCCTTGTGTCACAGTCTTCTGTGGATGGAAGTCCCAGCCAGTCATGGCTTCACCTATACCACACTAGTCATTTGTCCCCCTCAGTGTCCCAGTCACACAGTAGCATTTCCAGGGTGGCATCCCCAAGATCACTGTCTCCAACTACACCAACTAAGCAGAGGAGGGGCAGTAACATTGTCACATTACGGAAAGATGCTGGCCACCACAGAGGCCAACAAAGTAGCCCCATAGCGGGACACAGAAATAGGGGCATCCTGATTTCCCCAGCAAAGTCTCCACCCATTGACCTCACACAGTCAGTACCCGAGCCCCTGAGCCCCAGGGCCCAGGATCCTCTGCATTTCGTGAAAAAGGAGGATGAGGTCATCCTGTTACTGGACTCAGATGAGGAGCTGGAGCACACCAAGACAGAGTCAGTTTCTAAAGATTCCCCAGAAGGAAGGAAAGTTCCAGAATTTAGCCCCAGGTCCTCTGAACTGTTTTCAGTCATTGATGTTGAAGAAGATCATGAACATTTCCAAAGCCCACTGAAGAGAGAGGCCGGGCTACAGCACGGGGAGGAAGGACAGCTGGGAAACCAGTCTGCGCTGGGCTGCAGAGACATCCCCTGGCTGCTCTGCAGCCAGAAAACCAGCCTGGATGAGGACAGTGCTACAGACACCTCTTGGCTGGTGCCTGCCACTCCAGGGGTCAGCAGGAGTCGTGATTGCTCATCACAGACCCAAATCAAAAGCCTTAAGACCAGGATTCCGTCAGATGAGACTGCTCAGCAAACACCTAGACCTAACTTAGAACGCAGGACCATGCTGGAAACAGCCCAGCAGTTCTCTGTGATCATGCCACACACACAGCCTATTACTCTAGGAGCCTTTGACAGCGGAAGACAGGCCTACAGAAGCCCTTCCCATCCCTACCCCAGGCACCACAGACTCTCTTCTTCACAGCCATCATGTCCTGGCCCTGATTTTACCAGGTGGTCCCAGAAATCTTCCGCACCTAGACCATGCCTGCCAAATCTGCCTGCAGCTGATGATGTGGTAGAAGTTGGGGACAGTGATGATGAGGTGGCTTCCCATCAAGGAAACAGCAGCCCCGTGCTAGATGGTGACCCCCCAGGTCCCATGGGTGACTACTGTTGGAATGAGCCTCTCTCACCAATTCCAATTGACCACTTGAATCTGGAGCGGACCGGCCCCCTGACCACAAGCAGTCCCAGCAGTCAGGTCCTGGAGGCTTTGCACAGTGATGACTGCCACTCCCCGGGACTTGGTACCACCCCCATCCGAGGAAGCTGTGGTACGCTGAGAGAGTCTCAGGAACGGTCCTCACTGGCTGGCTCTCCAGAGGCTCTGTGGGACGACTGGAATGAGGAGGAAGGACAGTCTCCAGAGGCCCCACCTGTGGCTCAAATGCTGAGTACCAGAACTCGGAAGCCAGACAGGCCAGAGACACCAAAAGGTGCTAACCAGAAAAAGAACTTGCCTCCCAAAGTGCCTATAACTCCAATGCCAAGGTATTCGATCATGGAGACCCCGGTGCTGAAGAAAGAACTGGACAGGTTTGGAGTCCGTGCTCTGCCCAAACGCCAGATGGTTCTAAAACTGAAGGAGATTTTCCAGTACACTCACCAGACTCTGGAGTCGGACTCTGAGGATGAGGTCCAGTCCCCCCAGATACCCGCAGAACTACCTTGTCGCCAGGCATCTACCACTGAGACCTGCAACCCTTCAAGGTTGCCAACTGGGGAGCCTTCACATCCTGATGGTGACGCCCAGCTCCCAGCCTCCCAGGAATCCATGGCTACCTCTGTGGACGGCAGCGACAACTCCTTTAGCTCTAAAAGCTCCTCTGCTGAGTTTGGAGCTGCGTTTGAGTATTCAGATGAAGACAAGGATGAGGAGGTCGGGGTCACTGCATCACAGGCCGCAATCCAGGCCGCAGACACAGAGGAGGCAGTGAGGCGCTATATCCGCTCTAAGCCTGCCCTGCACAGGCAGGTCCTGAGGTACCAGCCTGTGGAGCTGGCCGAGCTGCAGGCTGAGCTGAAACAGAATGGCATCCCTGTGGCCATGGGCAAGCTGTCGGACATCCTGGACGCTCAGTGCATCACCTTCACCACTGCTGCAGCCCGAAAGGAGAAACTCAAACACAAGAGACGGCAGCCCTCAGGCCGAAAGAAAAAGGATCAGAAATGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMVPESAPNGNSQPLPSCFTTTGVPSPSKPRVSELVLQRMKQFKRADPERLRHASEESPQKTALGDDVPRSPPEETVGENEYKLDATDSDAAMALALQQEFRREEASSHHDSLEEKGLFFCQMCQKNLSAMNVTRREQHVNRCLDEAEKAQRPASPRIPDCPICGKPFLTTKSRISHLKQCAVRMEVGPQLLLQAVRLQTAQPEVDGSPQVPSFSNNVGGLKRKGVTTKREPRRRKVNKPEAPSEDLLVAMALSRSEVEHCPVVPPLRLENAFSEKIRLGAEKKSRKKRPPVCPPQLVTQDSETTGRQIEDRVAQLLSEEAELSCTPPLLASKISKEELEPAGWRARLPEGKRNFLWELSALTGAWAEESFYTVGLFPPIVSQCPSKEPQLPLELPKQGEPSPRRPPASQSSLPVSHSPKIRLLSSSQRERQALQDLVDLAVEGLSSSPQPGSRGVPTGLDLVPSSLPLTGFVLPCKKTLKKDDSASLSLGLLVTDFGAMVNNPHLSDVQFQLDSGEVLYAHKFVLYARCPLLIQYVSTESFSSEEDGDLTQRALLSDVSSEAAHAFLNYLYMADTDMPPSLVPDLRSLALRFGVSDLVQLCEQVPAVVDLEGEQPEETSEDCESRAETFLELLRSVWVDNEEEVETLLKPELCEEERERVNEAEMEEIYEFAATQRKLLQWGRAADPDGSTNPHGEDGAVSEPSLAGVQSNRQLENTEHMESSGLEKEEALASWEQEGHSTPLQDQCPDWAGKAEAQDALGEATDDPSFCSRHRRGKECLPLHPNKAHGCKQPLPSNPRVSSELSQITVDHEEQSDHVRETQADMAQAPTPHSCSLVSQSSVDGSPSQSWLHLYHTSHLSPSVSQSHSSISRVASPRSLSPTTPTKQRRGSNIVTLRKDAGHHRGQQSSPIAGHRNRGILISPAKSPPIDLTQSVPEPLSPRAQDPLHFVKKEDEVILLLDSDEELEHTKTESVSKDSPEGRKVPEFSPRSSELFSVIDVEEDHEHFQSPLKREAGLQHGEEGQLGNQSALGCRDIPWLLCSQKTSLDEDSATDTSWLVPATPGVSRSRDCSSQTQIKSLKTRIPSDETAQQTPRPNLERRTMLETAQQFSVIMPHTQPITLGAFDSGRQAYRSPSHPYPRHHRLSSSQPSCPGPDFTRWSQKSSAPRPCLPNLPAADDVVEVGDSDDEVASHQGNSSPVLDGDPPGPMGDYCWNEPLSPIPIDHLNLERTGPLTTSSPSSQVLEALHSDDCHSPGLGTTPIRGSCGTLRESQERSSLAGSPEALWDDWNEEEGQSPEAPPVAQMLSTRTRKPDRPETPKGANQKKNLPPKVPITPMPRYSIMETPVLKKELDRFGVRALPKRQMVLKLKEIFQYTHQTLESDSEDEVQSPQIPAELPCRQASTTETCNPSRLPTGEPSHPDGDAQLPASQESMATSVDGSDNSFSSKSSSAEFGAAFEYSDEDKDEEVGVTASQAAIQAADTEEAVRRYIRSKPALHRQVLRYQPVELAELQAELKQNGIPVAMGKLSDILDAQCITFTTAAARKEKLKHKRRQPSGRKKKDQK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU33967
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag; ; Estimated MW of expressed protein : 176127.92 Daltons; ; All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.