cDNA Clone TLK1

• Plasmid: pcDNA5-FRT/TO-GFP TLK1
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-TLK1
• Genbank: NM_012290.4
• Tag: GFP
• Species: Human

Sequence of Insert

AGATCTATGAGTGTCCAAAGTAGCAGTGGAAGTTTGGAGGGGCCGCCATCTTGGTCCCAGCTCTCCACGTCTCCAACCCCGGGCTCGGCGGCGGCGGCCAGGTCCCTGCTGAATCACACGCCGCCATCCGGGAGGCCCAGGGAAGGTGCAATGGATGAGCTTCATAGTCTGGATCCAAGAAGGCAAGAGTTATTGGAAGCTAGATTTACTGGAGTTGCAAGTGGGAGCACTGGAAGTACGGGCAGTTGCAGTGTTGGAGCTAAAGCCTCAACAAATAACGAAAGCTCTAATCACAGTTTTGGAAGCTTGGGATCTTTAAGTGACAAAGAATCAGAGACACCGGAGAAGAAACAATCGGAATCATCCAGGGGAAGAAAGAGAAAAGCAGAAAACCAGAATGAAAGTAGTCAGGGAAAAAGTATTGGGGGACGTGGCCACAAAATTAGCGACTATTTTGAATACCAGGGTGGAAATGGCTCAAGTCCAGTAAGAGGCATACCTCCTGCAATCCGTTCTCCTCAAAATTCACATTCACATTCCACTCCTTCCTCATCTGTTCGACCGAATAGCCCTTCTCCTACTGCATTAGCATTTGGGGACCACCCTATTGTACAACCAAAGCAATTATCCTTTAAAATTATTCAGACTGATCTCACAATGCTGAAATTAGCAGCATTAGAAAGTAATAAAATCCAGGACCTGGAAAAGAAGGAGGGACGTATAGATGATTTGCTCAGGGCTAACTGTGATCTCAGACGGCAAATAGATGAACAACAAAAATTACTTGAAAAATACAAAGAACGATTAAATAAGTGCATATCAATGAGCAAGAAACTTCTTATTGAAAAGAGTACACAAGAAAAGCTGTCAAGCAGAGAGAAGAGTATGCAAGATCGATTACGCCTTGGGCACTTTACAACAGTTAGACATGGCGCTTCATTTACTGAACAATGGACAGATGGTTTTGCATTTCAGAATCTTGTGAAGCAACAAGAATGGGTGAATCAGCAAAGGGAAGATATTGAAAGGCAAAGGAAACTTCTAGCCAAACGCAAACCTCCCACAGCTAATAATTCTCAGGCACCCTCTACCAATTCTGAACCAAAACAAAGGAAAAACAAAGCAGTCAATGGAGCAGAGAATGATCCCTTTGTTAGACCAAATTTACCACAACTGTTGACTTTGGCAGAATATCATGAACAGGAAGAAATTTTCAAACTTAGACTAGGACATCTCAAAAAGGAAGAGGCAGAAATCCAGGCAGAACTTGAACGTTTGGAAAGAGTCAGAAATCTTCACATACGTGAGCTGAAAAGAATAAACAATGAAGATAATTCACAGTTCAAAGATCACCCAACATTAAATGAAAGATATTTATTACTTCATCTGCTTGGTAGAGGTGGCTTTAGTGAAGTGTATAAGGCTTTTGACCTTTATGAACAAAGATATGCTGCTGTGAAGATACATCAGCTTAATAAAAGCTGGAGAGATGAGAAGAAAGAAAACTACCACAAACATGCCTGCAGAGAGTATAGAATACACAAAGAACTGGATCACCCCAGAATAGTTAAACTCTATGATTATTTCTCCTTGGATACAGATACGTTTTGTACAGTGTTAGAATACTGTGAAGGCAATGACTTGGATTTCTATCTGAAGCAACACAAGTTAATGTCAGAGAAAGAAGCTCGGTCTATTGTAATGCAGATTGTAAATGCACTAAGATATCTCAATGAGATCAAACCCCCTATTATACATTATGATCTTAAGCCAGGAAACATCCTACTGGTAGATGGAACAGCATGTGGTGAAATCAAAATCACTGATTTTGGTCTGTCCAAGATTATGGATGATGATAGCTATGGTGTAGATGGAATGGATCTAACTTCCCAGGGGGCAGGCACTTACTGGTATTTACCTCCTGAGTGTTTTGTAGTTGGAAAAGAGCCACCAAAGATTTCCAACAAGGTTGATGTATGGTCGGTTGGAGTCATCTTCTTTCAGTGTCTTTATGGTAGAAAGCCATTTGGTCACAATCAATCTCAACAAGACATTCTTCAAGAAAATACAATATTAAAAGCCACAGAAGTCCAGTTCCCTGTAAAACCGGTTGTAAGCAGTGAAGCCAAGGCATTTATAAGACGCTGTTTGGCATATCGAAAAGAAGATCGATTTGATGTGCACCAGCTGGCAAATGACCCATACCTTCTCCCACACATGAGAAGATCAAATTCTTCAGGAAACCTACACATGGCTGGGCTGACAGCATCCCCTACACCCCCTTCTTCAAGCATAATTACTTACTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMSVQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLLNHTPPSGRPREGAMDELHSLDPRRQELLEARFTGVASGSTGSTGSCSVGAKASTNNESSNHSFGSLGSLSDKESETPEKKQSESSRGRKRKAENQNESSQGKSIGGRGHKISDYFEYQGGNGSSPVRGIPPAIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQTDLTMLKLAALESNKIQDLEKKEGRIDDLLRANCDLRRQIDEQQKLLEKYKERLNKCISMSKKLLIEKSTQEKLSSREKSMQDRLRLGHFTTVRHGASFTEQWTDGFAFQNLVKQQEWVNQQREDIERQRKLLAKRKPPTANNSQAPSTNSEPKQRKNKAVNGAENDPFVRPNLPQLLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRINNEDNSQFKDHPTLNERYLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIVNALRYLNEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVDGMDLTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATEVQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVHQLANDPYLLPHMRRSNSSGNLHMAGLTASPTPPSSSIITY*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5-FRT/TO-GFP TLK168.34 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU33029
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression of protein with N-ter GFP tag; ; Estimated MW of expressed protein : 114024.76 Daltons
• Low Temp: No