cDNA Clone UHRF1

• Plasmid: pCMV5-HA UHRF1
• Parent Plasmid: pCMV5 HA2
• Expressed: HA-UHRF1
• Genbank: NM_001048201
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCTATGTGGATCCAGGTTCGGACCATGGATGGGAGGCAGACCCACACGGTGGACTCGCTGTCCAGGCTGACCAAGGTGGAGGAGCTGAGGCGGAAGATCCAGGAGCTGTTCCACGTGGAGCCAGGCCTGCAGAGGCTGTTCTACAGGGGCAAACAGATGGAGGACGGCCATACCCTCTTCGACTACGAGGTCCGCCTGAATGACACCATCCAGCTCCTGGTCCGCCAGAGCCTCGTGCTCCCCCACAGCACCAAGGAGCGGGACTCCGAGCTCTCCGACACCGACTCCGGCTGCTGCCTGGGCCAGAGTGAGTCAGACAAGTCCTCCACCCACGGTGAGGCGGCAGCCGAGACTGACAGCAGGCCAGCCGATGAGGACATGTGGGATGAGACGGAATTGGGGCTGTACAAGGTCAATGAGTACGTCGATGCTCGGGACACGAACATGGGGGCGTGGTTTGAGGCGCAGGTGGTCAGGGTGACGCGGAAGGCCCCCTCCCGGGACGAGCCCTGCAGCTCCACGTCCAGGCCGGCGCTGGAGGAGGACGTCATTTACCACGTGAAATACGACGACTACCCGGAGAACGGCGTGGTCCAGATGAACTCCAGGGACGTCCGAGCGCGCGCCCGCACCATCATCAAGTGGCAGGACCTGGAGGTGGGCCAGGTGGTCATGCTCAACTACAACCCCGACAACCCCAAGGAGCGGGGCTTCTGGTACGACGCGGAGATCTCCAGGAAGCGCGAGACCAGGACGGCGCGGGAACTCTACGCCAACGTGGTGCTGGGGGATGATTCTCTGAACGACTGTCGGATCATCTTCGTGGACGAAGTCTTCAAGATTGAGCGGCCGGGTGAAGGGAGCCCCATGGTTGACAACCCCATGAGACGGAAGAGCGGGCCGTCCTGCAAGCACTGCAAGGACGACGTGAACAGACTCTGCCGGGTCTGCGCCTGCCACCTGTGCGGGGGCCGGCAGGACCCCGACAAGCAGCTCATGTGCGATGAGTGCGACATGGCCTTCCACATCTACTGCCTGGACCCGCCCCTCAGCAGTGTTCCCAGCGAGGACGAGTGGTACTGCCCTGAGTGCCGGAATGATGCCAGCGAGGTGGTACTGGCGGGAGAGCGGCTGAGAGAGAGCAAGAAGAAGGCGAAGATGGCCTCGGCCACATCGTCCTCACAGCGGGACTGGGGCAAGGGCATGGCCTGTGTGGGCCGCACCAAGGAATGTACCATCGTCCCGTCCAACCACTACGGACCCATCCCGGGGATCCCCGTGGGCACCATGTGGCGGTTCCGAGTCCAGGTCAGCGAGTCGGGTGTCCATCGGCCCCACGTGGCTGGCATACACGGCCGGAGCAACGACGGAGCGTACTCCCTAGTCCTGGCGGGGGGCTATGAGGATGACGTGGACCATGGGAATTTTTTCACATACACGGGTAGTGGTGGTCGAGATCTTTCCGGCAACAAGAGGACCGCGGAACAGTCTTGTGATCAGAAACTCACCAACACCAACAGGGCGCTGGCTCTCAACTGCTTTGCTCCCATCAATGACCAAGAAGGGGCCGAGGCCAAGGACTGGCGGTCGGGGAAGCCGGTCAGGGTGGTGCGCAATGTCAAGGGTGGCAAGAATAGCAAGTACGCCCCCGCTGAGGGCAACCGCTATGATGGCATCTACAAGGTTGTGAAATACTGGCCCGAGAAGGGGAAGTCCGGGTTTCTCGTGTGGCGCTACCTTCTGCGGAGGGACGATGATGAGCCTGGCCCTTGGACGAAGGAGGGGAAGGACCGGATCAAGAAGCTGGGGCTGACCATGCAGTATCCAGAAGGCTACCTGGAAGCCCTGGCCAACCGAGAGCGAGAGAAGGAGAACAGCAAGAGGGAGGAGGAGGAGCAGCAGGAGGGGGGCTTCGCGTCCCCCAGGACGGGCAAGGGCAAGTGGAAGCGGAAGTCGGCAGGAGGTGGCCCGAGCAGGGCCGGGTCCCCGCGCCGGACATCCAAGAAAACCAAGGTGGAGCCCTACAGTCTCACGGCCCAGCAGAGCAGCCTCATCAGAGAGGACAAGAGCAACGCCAAGCTGTGGAATGAGGTCCTGGCGTCACTCAAGGACCGGCCGGCGAGCGGCAGCCCGTTCCAGTTGTTCCTGAGTAAAGTGGAGGAGACGTTCCAGTGTATCTGCTGTCAGGAGCTGGTGTTCCGGCCCATCACGACCGTGTGCCAGCACAACGTGTGCAAGGACTGCCTGGACAGATCCTTTCGGGCACAGGTGTTCAGCTGCCCTGCCTGCCGCTACGACCTGGGCCGCAGCTATGCCATGCAGGTGAACCAGCCTCTGCAGACCGTCCTCAACCAGCTCTTCCCCGGCTACGGCAATGGCCGGTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMWIQVRTMDGRQTHTVDSLSRLTKVEELRRKIQELFHVEPGLQRLFYRGKQMEDGHTLFDYEVRLNDTIQLLVRQSLVLPHSTKERDSELSDTDSGCCLGQSESDKSSTHGEAAAETDSRPADEDMWDETELGLYKVNEYVDARDTNMGAWFEAQVVRVTRKAPSRDEPCSSTSRPALEEDVIYHVKYDDYPENGVVQMNSRDVRARARTIIKWQDLEVGQVVMLNYNPDNPKERGFWYDAEISRKRETRTARELYANVVLGDDSLNDCRIIFVDEVFKIERPGEGSPMVDNPMRRKSGPSCKHCKDDVNRLCRVCACHLCGGRQDPDKQLMCDECDMAFHIYCLDPPLSSVPSEDEWYCPECRNDASEVVLAGERLRESKKKAKMASATSSSQRDWGKGMACVGRTKECTIVPSNHYGPIPGIPVGTMWRFRVQVSESGVHRPHVAGIHGRSNDGAYSLVLAGGYEDDVDHGNFFTYTGSGGRDLSGNKRTAEQSCDQKLTNTNRALALNCFAPINDQEGAEAKDWRSGKPVRVVRNVKGGKNSKYAPAEGNRYDGIYKVVKYWPEKGKSGFLVWRYLLRRDDDEPGPWTKEGKDRIKKLGLTMQYPEGYLEALANREREKENSKREEEEQQEGGFASPRTGKGKWKRKSAGGGPSRAGSPRRTSKKTKVEPYSLTAQQSSLIREDKSNAKLWNEVLASLKDRPASGSPFQLFLSKVEETFQCICCQELVFRPITTVCQHNVCKDCLDRSFRAQVFSCPACRYDLGRSYAMQVNQPLQTVLNQLFPGYGNGR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU19658
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N-ter HA.; ; Estimated MW of expressed protein : 92178.82 Daltons