cDNA Clone TRIM65

• Plasmid: pGEX6P-1-TRIM65d2
• Parent Plasmid: pGEX6P1
• Expressed: GST-TRIM65d2
• Genbank: NM_173547 (wild type)
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGGCCGCGCAGCTGCTGGAGGAGAAGCTGACCTGCGCCATCTGCCTGGGGCTCTACCAGGACCCAGTGACGCTGCCCTGCGGCCACAACTTCTGCGGGGCCTGCATCCGGGACTGGTGGGACCGCTGCGGAAAGGCGTGCCCCGAGTGCCGGGAGCCCTTTCCCGACGGCGCCGAGCTGCGCCGCAACGTGGCCCTCAGCGGCGTGCTGGAGGTGGTGCGCGCCGGGCCCGCCCGGGATCCCGGCCCCGATCCCGGCCCCGGCCCCGACCCTGCCGCGCGCTGCCCCCGCCACGGGCGGCCGCTGGAGCTCTTCTGCCGGACCGAGGGCCGCTGTGTGTGCAGCGTGTGCACCGTGCGCGAGTGTCGCCTCCACGAGCGGGCGCTGCTGGATGCCGAGCGCCTCAAGCGCGAGAACTCGGCCTGCATCTTGGCCTCCTGGGTCTCCGGCAAGTTCAGCAGCCTGCTACAGGCCCTGGAAATACAGCACACGACAGCACTGAGGAGCATCGAGGTGGCCAAGACGCAGGCGCTGGCACAGGCTCGAGACGAGGAGCAGCGGCTGCGGGTCCATTTGGAGGCTGTGGCTCGCCATGGCTGCAGGATCCGGGAGCTCCTGGAGCAGGTGGATGAGCAGACCTTCCTGCAGGAATCGCAGCTCCTCCAGCCCCCAGGGCCTCTTGGGCCACTGACCCCTCTGCAGTGGGATGAAGACCAACAGCTGGGTGACCTGAAGCAGTTGCTAAGCCGGCTGTGTGGCCTCCTCTTGGAAGAGGGGAGCCACCCTGGGGCACCAGCCAAGCCTGTGGACTTAGCCCCCGTGGAGGCCCCAGGTCCCCTGGCACCGGTCCCAAGCACAGTTTGTCCACTGAGGAGGAAACTCTGGCAGAATTATCGCAATCTGACCTTTGATCCAGTCAGCGCCAACCGTCACTTCTATCTGTCGCGCCAGGACCAGCAGGTGAAGCACTGTCGTCAGTCCCGGGGCCCAGGCGGGCCCGGCAGCTTTGAGCTCTGGCAGGTGCAATGTGCCCAGAGCTTCCAGGCCGGGCACCACTACTGGGAGGTGCGCGCGTCAGACCACTCGGTGACACTGGGCGTCTCCTACCCGCAACTGCCACGGTGCAGGCTGGGGCCCCACACAGACAACATTGGCCGGGGACCCTGCTCCTGGGGGCTCTGCGTCCAGGAGGACAGCCTCCAGGCCTGGCACAACGGGGAAGCCCAGCGCCTCCCAGGGGTGTCAGGGCGGCTCCTGGGCATGGATTTGGACCTGGCCTCAGGCTGCCTCACCTTCTACAGCCTGGAGCCCCAGACCCAGCCCCTGTACACCTTCCATGCCCTCTTCAACCAGCCCCTCACCCCCGTCTTCTGGCTCCTCGAGGGTAGGACCCTGACCCTGTGCCATCAGCCAGGGGCTGTGTTCCCTCTGGGGCCCCAGGAAGAGGTGCTCAGCTGAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAAQLLEEKLTCAICLGLYQDPVTLPCGHNFCGACIRDWWDRCGKACPECREPFPDGAELRRNVALSGVLEVVRAGPARDPGPDPGPGPDPAARCPRHGRPLELFCRTEGRCVCSVCTVRECRLHERALLDAERLKRENSACILASWVSGKFSSLLQALEIQHTTALRSIEVAKTQALAQARDEEQRLRVHLEAVARHGCRIRELLEQVDEQTFLQESQLLQPPGPLGPLTPLQWDEDQQLGDLKQLLSRLCGLLLEEGSHPGAPAKPVDLAPVEAPGPLAPVPSTVCPLRRKLWQNYRNLTFDPVSANRHFYLSRQDQQVKHCRQSRGPGGPGSFELWQVQCAQSFQAGHHYWEVRASDHSVTLGVSYPQLPRCRLGPHTDNIGRGPCSWGLCVQEDSLQAWHNGEAQRLPGVSGRLLGMDLDLASGCLTFYSLEPQTQPLYTFHALFNQPLTPVFWLLEGRTLTLCHQPGAVFPLGPQEEVLS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU19441
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express TRIM65 with N-ter GST.