cDNA Clone SLD3 (yeast)

• Plasmid: pRS415 SLD3 c-6H3M
• Parent Plasmid: pRS415
• Genbank: NP_011402.1
• Species: Yeast

Sequence of Insert

GGATCCTCCGAGTGAGCAATTCAGGATACCGACAGCAGTAGTGTGGATAGATTGTGCAAGACTAGTAACTGGTAAAGGGCTCCCTGATAAGGCCTTGGAGTGCTCCATGATTCTCGGAGTCATATTTGCCGTTTTATCATTAATCAGAAACACTTATAGAGATTACGGATACGGGTGGATATTATATATTCCGTCTGGTGTAGCAGTCGGTGTTGGTATATTTAATTCTCCCAGTTTTACAATTGCAAGATTCATCGGCGGGTGGGCTTCGCATTTTTGGTTGAAGAATCATAGGGGTGACTTAAATGCGAAAACAAAAATGATTGTATTCAGTTCGGGGTTGGTCTTAGGTGAAGGTATCTTTAGCGTAATAAACATGCTCTTCATCTGCTTAAATGTCCCTCACTATTAGGTATATAATATTTTACTAATAAAGAATTTACAAAGTTTACAAATTTTAAATTATTTACTCATCACATAATTCGATGTCTTAGATTTCCCTGTATATATATTTCTAGGTGACAAAAATCCTAGGCAAAATGTTATATTATACGCGACCTTGAAATATCGGCTATCTTGGTGTACAACATGGTCCATTTAAGAAAAATAAAAAAAAGTGATGGGCACTGTTAAAACAGGCAGAAGCCTCATTCATAACTGCTGAAGGGTTAAGATCGTATAGGTAGGAACGATCTGACCTCTCATAATGGAAACATGGGAAGTCATAGCATCGGTAAAAGAAGCAACAAAAGGCCTTGACTTGAGCTTAGATCATCCACTTATTATTAAATCCGAGGACGTGCCCAGTAACATTCTCCAATTACTGCAACAGAAGAATCGACGTCAATTGAAACACATCTGCATGAAATCAAGAAAAGAGTATTTTCTACTAGAGGAATATGGGCCAGGATTTTGGGTTAAGTGGCCGTACAATTATTTCAATGGTTACAGCTTACCAGAAAGGCGTACGGAAGTTGTAACGACAGTTGAAAGAGAAAGAGCTAAGCGTGAAACGTTAAAAACATGGGACGAATTGAAATTTAAAGAGCTTCTTCATTTATGGTCAGAAGAACCCAAGGGCTCGTGTAAGCTTGAGAAGGACAAAGACCTTAAACTGGATATGAATCCCCCAGATATGAAAGGCGAATCAAAAATTAATGACTACTATTCAGACCCTAAAGAGTACATAGAAAGTAAGTATTATGATGCTCTTTTTTCCATACATACGCCTCTCGCATATTTCGTCAAGTCAAATTTAGTAAGGCTCAAAAATACCTGCCGAACCAAGTACGGAAGTGACAGTTACAAAATAGCCTATCAAGCCATGCTGCAAAAATTTCTTCTCTCAATTGTACAATTCAAAGATAGACATGATAACAGGCTTTTATTAGAGCCCTTTTCTAGTCCCATAGCAGATGAAAAAAGGAAGAACTGTCTCACAAAATTTGTTATCCAAGATGAAAACAAAAACAGCTCAACCATTGCTGATTTATGTGTTGTATTAAAATCCCGCGAAATAAAGCTACAAATATTATTGCTATTAGAGATAATAGGATTGAACGATTTAGATTGGAATTTTAGAGATTTTGAGAAAAAGTATAAATTAAAATTGAAGAAAAGATCACTTAATTTGACAAAAAAGGGATTAGTTCGTCGAAGATCGAAGAAAAAAACCAGCGAAAAAGACAAAGGAATCGAGAGAATAACAACATCTTTGGATTATTGTGAACAGTTAGACTTGTACTTAGATAGAGCATGCATCTTGGACATTCTACTATCAAGTGAAACGCCCAACCCAGATGCCATAGAAGCATCAAATGGAACAATACAAGAGCATAAAAAAAATATCCTAGACAAAAGCAAAGAAGCCTCATTGGTTGGGTTCATAAATTATGTTCTTATTCCATATTTCAACAAAAAGGTACCACACGCCGTTGAATTTATAATTCAAAAGCTCAAGGGACCAAGCATGAGACCAAAGAGAGCCCTGAAAAAGGTCAACGATAGCACAAATGTATCGTCACCTAATACTGTAGAAACGTATAACAGGTTATCCACGAGTCAACGTGCCTCTCGCTCTTCAATCATTAATTCCGTCCCATCTTCACCCGCATTGAGAAGGGTGGACGCTAATTTGTTCAGCAGAAAATCTATAGCTTCGCCCACCCCTGAACTTTTGAATTCTAGAACGAACTCTAACTTGAATGAATTTCTCGAAAGTGAAACTAGAAGCTTAAAGCGCCCTTCACAATTGGGAAGGACGAAGTCTGATCTAACGATGAATCATTTACAAAAACGGCAGTTTTCTGTCTCTGACTTAAGCACAACAAGAGTACCGAATTCATCAACTATCACACTCAAAACGCCTTTCTCGCACTCAACTATTAATGCATACAAAACTATGAATAACTCTTTTCGCAGAGTTGGGAAACGTAAAGATATCAATGAAACGATACGCCTACATGAACGTGTAGACTCTGAGGAAAACGTACAAGTTCAAGCCACTCCTGCTGTGAAAAAAAGAACTGTGACACCTAATAAAAAGGCGCAACTTCAAAGCATCATTGAATCGCCGCTAAATTTCAAGGATGATGATACGCATGAAGGCAGAAAAAATACCTCCAATATTACCTCTACTCCCACTAATAAGCCCCCGGAAAATAGCTCAAAAAGGAGAGTAAGAAGACGTTTATTTGCTCCAGAATCCACAGCGGCCGC

Amino Acid Sequence

METWEVIASVKEATKGLDLSLDHPLIIKSEDVPSNILQLLQQKNRRQLKHICMKSRKEYFLLEEYGPGFWVKWPYNYFNGYSLPERRTEVVTTVERERAKRETLKTWDELKFKELLHLWSEEPKGSCKLEKDKDLKLDMNPPDMKGESKINDYYSDPKEYIESKYYDALFSIHTPLAYFVKSNLVRLKNTCRTKYGSDSYKIAYQAMLQKFLLSIVQFKDRHDNRLLLEPFSSPIADEKRKNCLTKFVIQDENKNSSTIADLCVVLKSREIKLQILLLLEIIGLNDLDWNFRDFEKKYKLKLKKRSLNLTKKGLVRRRSKKKTSEKDKGIERITTSLDYCEQLDLYLDRACILDILLSSETPNPDAIEASNGTIQEHKKNILDKSKEASLVGFINYVLIPYFNKKVPHAVEFIIQKLKGPSMRPKRALKKVNDSTNVSSPNTVETYNRLSTSQRASRSSIINSVPSSPALRRVDANLFSRKSIASPTPELLNSRTNSNLNEFLESETRSLKRPSQLGRTKSDLTMNHLQKRQFSVSDLSTTRVPNSSTITLKTPFSHSTINAYKTMNNSFRRVGKRKDINETIRLHERVDSEENVQVQATPAVKKRTVTPNKKAQLQSIIESPLNFKDDDTHEGRKNTSNITSTPTNKPPENSSKRRVRRRLFAPESTAAAHHHHHHGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLG*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU19032
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression plasmid to express SLD3 with 6xHIS and 3xMyc c-terminal tags.