cDNA Clone POL12 (yeast)

• Plasmid: pRS415 POL12 c-6H3M
• Parent Plasmid: pRS415
• Genbank: NP_009518
• Species: Yeast

Sequence of Insert

ACTAGTAGAAGTATGGGAACAGATCCTTTTAATGCTTAATAAATTTTCTGATTATGGAAACGAAATCTATAAATTAGCGCAAGAGTTCAGGGACAGTCTAATGTTATCGCACTTTTTCAAAAAACATTCGGCAACAAGAATTCTAAGCATGTTGGTTACTGAAATTCAGCCTGACACCGCTGGTGTGAAAGAAACATTTTTAATTGAGACCTTATGGAAGATGCTGCAATCTCCTACCATTTGCCAACAATTCAAGAAATCTAATATATCAGAGATTATCCAAACAATGAGCTATTTCATTATGGGGACTGATAATACTTCGTGGAACTTCACCAGTGCCGTGGTATTAGCTCGTTGTTTGAGAGTCCTCCAAACTACTCCAGATTATACTGAGCAAGAAACCGAACGATTATTCGATTGTTTGCCTAAGAATGTCTTTAAAATGATCATGTTCATCGCCTCAAACGAATAGAGAATAGCGTGGTAGACGCGTAATTTTTTTTTACCGTGACGCGTCTCACCAGAGTTTCTTAGTTCTTTCTCTTTTCTAAAACTGTAATAATATGAGCTCTTTGTAGACACATATAACATTTTGTTTCATATACATAATATATTTGCATATAAAAATATCAAGATAATTGATTGTCAATTGGTTCAACAAAGCTTGAAGGTAAATTTAGGCAGGAGCAAGTGACTGAAAAAAAAAAAAATGAGCGGATCTATCGATGTCATCACTCATTTTGGGCCTGATGCAGACAAGCCGGAAATCATCACTGCATTAGAGAATTTAACTAAACTGCATGCATTGTCCGTCGAGGATCTATACATTAAATGGGAGCAGTTCTCCAATCAAAGGCGTCAAACTCACACAGATCTGACTTCCAAGAACATAGATGAGTTTAAACAATTTTTACAATTACAAATGGAAAAGCGTGCTAACCAAATTTCATCGTCCTCTAAGGTAAACACATCCACAAAAAAACCAGTGATTAAAAAAAGTTTGAATTCAAGTCCCTTGTTTGGCCTTAGTATTCCAAAGACTCCGACTTTAAAAAAAAGAAAGTTACATGGTCCCTTTTCACTCAGTGATTCCAAGCAAACATATAATGTAGGCTCTGAGGCAGAAACAAATGAAAAAGGAAATAGTTCTTTAAAATTGGAGTTTACTCCAGGCATGGCAGAAGATGCTGTGGGTGACAGTGCTCCATTGTCCCACGCTAAAAGCTCGGATGCCAAGACTCCCGGCTCGTCGACATTCCAAACTCCAACAACAAACACTCCTACCACATCGAGGCAAAATGTTCCTGCTGGTGAAATTTTGGATTCTTTGAACCCTGAAAATATTGAAATATCATCTGGTAATCCAAATGTAGGCCTTCTTTCGACTGAAGAACCATCATATAACCAGGTCAAAGTAGAACCATTTTATGATGCTAAAAAGTATAAATTCAGAACAATGAGGCAAAACTTACAGGAAGCGTCTGATGTCCTCGATGATCAAATTGAATCGTTTACTAAGATTATTCAAAATCACTATAAGTTATCTCCAAACGACTTCGCTGATCCAACTATTCAATCTCAGTCCGAAATTTATGCTGTTGGAAGGATAGTTCCAGATTCGCCGACCTACGACAAGTTTTTGAATCCAGAATCGCTTTCCCTAGAGACCTCAAGAATGGGCGGCGTTGGAAGAAGGGTAAGGTTAGATCTATCGCAGGTCAACGAGCTTTCGTTTTTCTTAGGCCAAATTGTGGCATTCAAAGGTAAAAATGCTAATGGGGATTACTTCACCGTTAACTCTATTTTGCCGCTACCTTATCCAAACTCCCCAGTGTCCACATCGCAGGAACTGCAGGAGTTTCAAGCAAATTTGGAAGGTTCTTCTTTGAAGGTTATCGTGACCTGTGGCCCATACTTTGCAAATGATAACTTCTCCCTCGAATTGTTGCAAGAATTTATCGATAGTATAAATAATGAGGTAAAACCTCACGTCTTGATAATGTTTGGCCCATTTATAGATATCACCCATCCTCTGATAGCGAGTGGTAAATTGCCAAATTTTCCCCAGTTCAAGACACAGCCCAAAACATTGGACGAGCTTTTTTTGAAGTTATTTACTCCTATTCTAAAAACCATATCACCACATATCCAAACCGTATTGATACCCTCCACAAAAGATGCAATTTCTAATCACGCCGCATATCCACAAGCTTCTTTAATTAGAAAGGCTTTACAGCTGCCTAAAAGGAACTTCAAATGCATGGCTAATCCTTCATCTTTTCAAATAAATGAGATATACTTCGGTTGCTCAAACGTTGATACATTCAAAGATCTAAAGGAAGTCATAAAAGGCGGTACTACCTCATCAAGATATAGATTAGATCGTGTTTCTGAGCATATTTTACAACAGCGCAGGTATTACCCCATATTTCCAGGTAGTATTCGTACAAGAATAAAACCAAAAGACGTATCTACGAAGAAGGAAACCAATGATATGGAAAGCAAAGAAGAAAAAGTTTATGAACATATATCTGGTGCAGATTTAGACGTGAGTTATCTGGGACTAACAGAGTTTGTTGGTGGGTTCTCGCCTGACATAATGATTATACCCAGTGAATTACAACACTTTGCAAGAGTTGTCCAAAACGTAGTTGTTATAAATCCCGGAAGATTTATTAGAGCAACAGGTAACAGGGGATCCTATGCACAAATTACTGTCCAATGCCCTGATCTTGAAGACGGGAAATTGACGCTTGTTGAAGGTGAAGAGCCAGTTTATCTACACAACGTGTGGAAGCGCGCTAGAGTTGACTTGATTGCTAGTGCGGCCGCACACCATCACCATCACCACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTGAACGGACTCGACGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGGGTTAAGCGGCCGC

Amino Acid Sequence

MSGSIDVITHFGPDADKPEIITALENLTKLHALSVEDLYIKWEQFSNQRRQTHTDLTSKNIDEFKQFLQLQMEKRANQISSSSKVNTSTKKPVIKKSLNSSPLFGLSIPKTPTLKKRKLHGPFSLSDSKQTYNVGSEAETNEKGNSSLKLEFTPGMAEDAVGDSAPLSHAKSSDAKTPGSSTFQTPTTNTPTTSRQNVPAGEILDSLNPENIEISSGNPNVGLLSTEEPSYNQVKVEPFYDAKKYKFRTMRQNLQEASDVLDDQIESFTKIIQNHYKLSPNDFADPTIQSQSEIYAVGRIVPDSPTYDKFLNPESLSLETSRMGGVGRRVRLDLSQVNELSFFLGQIVAFKGKNANGDYFTVNSILPLPYPNSPVSTSQELQEFQANLEGSSLKVIVTCGPYFANDNFSLELLQEFIDSINNEVKPHVLIMFGPFIDITHPLIASGKLPNFPQFKTQPKTLDELFLKLFTPILKTISPHIQTVLIPSTKDAISNHAAYPQASLIRKALQLPKRNFKCMANPSSFQINEIYFGCSNVDTFKDLKEVIKGGTTSSRYRLDRVSEHILQQRRYYPIFPGSIRTRIKPKDVSTKKETNDMESKEEKVYEHISGADLDVSYLGLTEFVGGFSPDIMIIPSELQHFARVVQNVVVINPGRFIRATGNRGSYAQITVQCPDLEDGKLTLVEGEEPVYLHNVWKRARVDLIASAAAHHHHHHGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLG*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU19013
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression plasmid to express POL12 with 6xHIS and 3xMyc c-terminal tags.