cDNA Clone PPM1E

• Plasmid: pGEX6P-1 6His-TEV-PPM1E D435N
• Parent Plasmid: pGEX6P1
• Expressed: GST P-6HIS TEV PPM1E D435N
• Genbank: NM_014906.4 GI:387157887 with silent changes g96a and g99c
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCATCACCATCACCATCACGATTACGATATCCCAACGACCGAAAACCTGTATTTTCAGATGGCCGGCTGCATCCCTGAGGAGAAAACTTACCGGCGCTTCCTGGAGCTATTCCTGGGCGAGTTTCGCGGACCGTGCGGCGGCGGCGAGCCGGAACCCGAACCCGAACCCGAACCCGAACCCGAACCCGAGTCCGAGCCCGAGCCCGAACCTGAACTGGTAGAAGCTGAGGCGGCCGAGGCTTCGGTAGAGGAACCCGGGGAGGAGGCGGCCACGGTAGCCGCGACGGAGGAGGGGGACCAGGAGCAAGACCCGGAGCCCGAGGAGGAGGCGGCGGTTGAGGGTGAGGAGGAGGAGGAGGGCGCGGCGACGGCGGCGGCAGCCCCGGGGCACTCGGCCGTGCCGCCGCCGCCGCCCCAGCTGCCGCCTTTGCCCCCGCTCCCGCGACCGCTGTCAGAGCGCATCACCCGCGAGGAGGTGGAGGGCGAAAGCCTGGACCTGTGCCTGCAGCAGCTCTACAAATATAATTGCCCTTCCTTTTTGGCTGCTGCTTTAGCCAGAGCCACATCAGATGAAGTCCTTCAGAGTGATCTTTCTGCACATTATATCCCAAAGGAAACGGATGGCACAGAAGGGACTGTGGAGATTGAGACAGTGAAATTGGCCCGTTCTGTCTTCAGCAAACTACACGAGATTTGCTGCAGCTGGGTGAAAGACTTCCCCCTCCGCAGGAGACCCCAGCTTTATTATGAGACATCAATCCATGCCATCAAAAACATGCGCAGGAAAATGGAGGACAAACATGTCTGCATTCCTGACTTTAATATGCTCTTCAACCTAGAGGACCAGGAAGAACAAGCTTACTTTGCAGTGTTTGATGGCCATGGGGGAGTAGATGCTGCTATTTATGCCTCCATTCACCTCCACGTTAACTTAGTCCGCCAGGAGATGTTCCCCCATGATCCTGCTGAGGCCCTGTGCAGGGCCTTCCGGGTCACTGATGAGCGGTTTGTGCAGAAAGCAGCCAGGGAGAGCTTAAGATGTGGGACCACAGGAGTGGTGACTTTCATCAGAGGCAACATGCTACATGTGGCCTGGGTGGGTGATTCCCAGGTTATGCTTGTGAGAAAGGGCCAAGCTGTTGAACTAATGAAGCCACACAAACCAGACAGAGAGGATGAAAAGCAGAGAATTGAGGCCCTTGGAGGTTGCGTAGTCTGGTTTGGTGCCTGGAGGGTGAATGGAAGTCTGTCGGTTTCCAGAGCTATTGGAGATGCTGAACATAAGCCATATATCTGTGGGGATGCAGATTCTGCCTCCACTGTTCTGGATGGGACCGAAGACTACCTCATTCTGGCCTGTAATGGCTTCTATGACACCGTGAACCCTGATGAGGCAGTGAAAGTTGTGTCCGACCACCTGAAAGAGAATAATGGAGACAGCAGCATGGTTGCCCACAAATTAGTGGCATCAGCTCGTGATGCTGGGTCAAGTGATAACATCACGGTTATTGTGGTATTCCTGAGGGACATGAACAAAGCTGTAAATGTTAGTGAGGAATCAGATTGGACAGAGAACTCTTTTCAAGGAGGGCAAGAAGATGGTGGGGATGATAAGGAGAATCATGGAGAGTGCAAACGCCCTTGGCCTCAGCACCAGTGCTCAGCACCAGCCGACCTAGGCTATGATGGGCGTGTGGATTCATTCACTGATAGAACTAGCCTGAGCCCAGGGTCCCAAATCAACGTGCTGGAAGACCCAGGCTACCTAGATCTCACACAAATAGAAGCAAGCAAACCTCACAGTGCCCAGTTTTTGCTACCAGTTGAGATGTTTGGTCCTGGTGCACCAAAGAAAGCAAATCTTATTAATGAGTTAATGATGGAGAAAAAATCAGTTCAGTCATCATTGCCTGAATGGAGTGGTGCTGGAGAGTTTCCCACTGCTTTCAATTTGGGTTCAACAGGGGAGCAGATATACAGAATGCAGAGCTTGTCTCCTGTCTGTTCAGGGTTGGAAAATGAACAGTTCAAATCCCCGGGAAACAGAGTTTCTAGATTGTCTCATTTACGCCACCACTACTCAAAGAAGTGGCACAGATTCAGGTTTAATCCAAAGTTTTATTCATTTCTCTCTGCTCAAGAGCCTTCCCACAAAATAGGCACTAGCCTGTCCTCACTTACTGGAAGTGGGAAGAGAAATAGGATAAGAAGTTCTCTGCCATGGAGGCAAAATAGTTGGAAAGGGTACAGTGAAAACATGAGGAAGCTCAGAAAGACTCATGATATTCCATGCCCAGATCTTCCTTGGAGCTATAAAATAGAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSHHHHHHDYDIPTTENLYFQ
MAGCIPEEKTYRRFLELFLGEFRGPCGGGEPEPEPEPEPEPEPESEPEPE
PELVEAEAAEASVEEPGEEAATVAATEEGDQEQDPEPEEEAAVEGEEEEE
GAATAAAAPGHSAVPPPPPQLPPLPPLPRPLSERITREEVEGESLDLCLQ
QLYKYNCPSFLAAALARATSDEVLQSDLSAHYIPKETDGTEGTVEIETVK
LARSVFSKLHEICCSWVKDFPLRRRPQLYYETSIHAIKNMRRKMEDKHVC
IPDFNMLFNLEDQEEQAYFAVFDGHGGVDAAIYASIHLHVNLVRQEMFPH
DPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDS
QVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVS
RAIGDAEHKPYICGDADSASTVLDGTEDYLILACNGFYDTVNPDEAVKVV
SDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRDMNKAVNVSEE
SDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFT
DRTSLSPGSQINVLEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKA
NLINELMMEKKSVQSSLPEWSGAGEFPTAFNLGSTGEQIYRMQSLSPVCS
GLENEQFKSPGNRVSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKI
GTSLSSLTGSGKRNRIRSSLPWRQNSWKGYSENMRKLRKTHDIPCPDLPW
SYKIE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35239
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee