cDNA Clone A20

• Plasmid: pMalP A20
• Parent Plasmid: pMAL6P
• Expressed: MBP A20
• Genbank: BC114480
• Tag: MBP
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCTGAACAAGTCCTTCCTCAGGCTTTGTATTTGAGCAA
TATGCGGAAAGCTGTGAAGATACGGGAGAGAACTCCAGAAGACATTTTTA
AACCTACTAATGGGATCATTCATCATTTTAAAACCATGCACCGATACACA
CTGGAAATGTTCAGAACTTGCCAGTTTTGTCCTCAGTTTCGGGAGATCAT
CCACAAAGCCCTCATCGACAGAAACATCCAGGCCACCCTGGAAAGCCAGA
AGAAACTCAACTGGTGTCGAGAAGTCCGGAAGCTTGTGGCGCTGAAAACG
AACGGTGACGGCAATTGCCTCATGCATGCCACTTCTCAGTACATGTGGGG
CGTTCAGGACACAGACTTGGTACTGAGGAAGGCGCTGTTCAGCACGCTCA
AGGAAACAGACACACGCAACTTTAAATTCCGCTGGCAACTGGAGTCTCTC
AAATCTCAGGAATTTGTTGAAACGGGGCTTTGCTATGATACTCGGAACTG
GAATGATGAATGGGACAATCTTATCAAAATGGCTTCCACAGACACACCCA
TGGCCCGAAGTGGACTTCAGTACAACTCACTGGAAGAAATACACATATTT
GTCCTTTGCAACATCCTCAGAAGGCCAATCATTGTCATTTCAGACAAAAT
GCTAAGAAGTTTGGAATCAGGTTCCAATTTCGCCCCTTTGAAAGTGGGTG
GAATTTACTTGCCTCTCCACTGGCCTGCCCAGGAATGCTACAGATACCCC
ATTGTTCTCGGCTATGACAGCCATCATTTTGTACCCTTGGTGACCCTGAA
GGACAGTGGGCCTGAAATCCGAGCTGTTCCACTTGTTAACAGAGACCGGG
GAAGATTTGAAGACTTAAAAGTTCACTTTTTGACAGATCCTGAAAATGAG
ATGAAGGAGAAGCTCTTAAAAGAGTACTTAATGGTGATAGAAATCCCCGT
CCAAGGCTGGGACCATGGCACAACTCATCTCATCAATGCCGCAAAGTTGG
ATGAAGCTAACTTACCAAAAGAAATCAATCTGGTAGATGATTACTTTGAA
CTTGTTCAGCATGAGTACAAGAAATGGCAGGAAAACAGCGAGCAGGGGAG
GAGAGAGGGGCACGCCCAGAATCCCATGGAACCTTCCGTGCCCCAGCTTT
CTCTCATGGATGTAAAATGTGAAACGCCCAACTGCCCCTTCTTCATGTCT
GTGAACACCCAGCCTTTATGCCATGAGTGCTCAGAGAGGCGGCAAAAGAA
TCAAAACAAACTCCCAAAGCTGAACTCCAAGCCGGGCCCTGAGGGGCTCC
CTGGCATGGCGCTCGGGGCCTCTCGGGGAGAAGCCTATGAGCCCTTGGCG
TGGAACCCTGAGGAGTCCACTGGGGGGCCTCATTCGGCCCCACCGACAGC
ACCCAGCCCTTTTCTGTTCAGTGAGACCACTGCCATGAAGTGCAGGAGCC
CCGGCTGCCCCTTCACACTGAATGTGCAGCACAACGGATTTTGTGAACGT
TGCCACAACGCCCGGCAACTTCACGCCAGCCACGCCCCAGACCACACAAG
GCACTTGGATCCCGGGAAGTGCCAAGCCTGCCTCCAGGATGTTACCAGGA
CATTTAATGGGATCTGCAGTACTTGCTTCAAAAGGACTACAGCAGAGGCC
TCCTCCAGCCTCAGCACCAGCCTCCCTCCTTCCTGTCACCAGCGTTCCAA
GTCAGATCCCTCGCGGCTCGTCCGGAGCCCCTCCCCGCATTCTTGCCACA
GAGCTGGAAACGACGCCCCTGCTGGCTGCCTGTCTCAAGCTGCACGGACT
CCTGGGGACAGGACGGGGACGAGCAAGTGCAGAAAAGCCGGCTGCGTGTA
TTTTGGGACTCCAGAAAACAAGGGCTTTTGCACACTGTGTTTCATCGAGT
ACAGAGAAAACAAACATTTTGCTGCTGCCTCAGGGAAAGTCAGTCCCACA
GCGTCCAGGTTCCAGAACACCATTCCGTGCCTGGGGAGGGAATGCGGCAC
CCTTGGAAGCACCATGTTTGAAGGATACTGCCAGAAGTGTTTCATTGAAG
CTCAGAATCAGAGATTTCATGAGGCCAAAAGGACAGAAGAGCAACTGAGA
TCGAGCCAGCGCAGAGATGTGCCTCGAACCACACAAAGCACCTCAAGGCC
CAAGTGCGCCCGGGCCTCCTGCAAGAACATCCTGGCCTGCCGCAGCGAGG
AGCTCTGCATGGAGTGTCAGCATCCCAACCAGAGGATGGGCCCTGGGGCC
CACCGGGGTGAGCCTGCCCCCGAAGACCCCCCCAAGCAGCGTTGCCGGGC
CCCCGCCTGTGATCATTTTGGCAATGCCAAGTGCAACGGCTACTGCAACG
AATGCTTTCAGTTCAAGCAGATGTATGGCTAAGCGGCCGC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSPEFPGVDSAAAMAEQVLPQALY
LSNMRKAVKIRERTPEDIFKPTNGIIHHFKTMHRYTLEMFRTCQFCPQFR
EIIHKALIDRNIQATLESQKKLNWCREVRKLVALKTNGDGNCLMHATSQY
MWGVQDTDLVLRKALFSTLKETDTRNFKFRWQLESLKSQEFVETGLCYDT
RNWNDEWDNLIKMASTDTPMARSGLQYNSLEEIHIFVLCNILRRPIIVIS
DKMLRSLESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSHHFVPLV
TLKDSGPEIRAVPLVNRDRGRFEDLKVHFLTDPENEMKEKLLKEYLMVIE
IPVQGWDHGTTHLINAAKLDEANLPKEINLVDDYFELVQHEYKKWQENSE
QGRREGHAQNPMEPSVPQLSLMDVKCETPNCPFFMSVNTQPLCHECSERR
QKNQNKLPKLNSKPGPEGLPGMALGASRGEAYEPLAWNPEESTGGPHSAP
PTAPSPFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHNARQLHASHAPD
HTRHLDPGKCQACLQDVTRTFNGICSTCFKRTTAEASSSLSTSLPPSCHQ
RSKSDPSRLVRSPSPHSCHRAGNDAPAGCLSQAARTPGDRTGTSKCRKAG
CVYFGTPENKGFCTLCFIEYRENKHFAAASGKVSPTASRFQNTIPCLGRE
CGTLGSTMFEGYCQKCFIEAQNQRFHEAKRTEEQLRSSQRRDVPRTTQST
SRPKCARASCKNILACRSEELCMECQHPNQRMGPGAHRGEPAPEDPPKQR
CRAPACDHFGNAKCNGYCNECFQFKQMYG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35224
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter MBP for bacterial expression; ; Estimated MW of expressed protein : 137391 Daltons