cDNA Clone PKNG

• Plasmid: pEGFP-C2-TEV PKNg
• Parent Plasmid: pEGFPC2
• Expressed: GFP TEV PKNg
• Genbank: ACT23439.1
• Tag: GFP
• Species: Mycobacterium tuberculosis KZN1435

Sequence of Insert

GAATTCGAGAATCTTTATTTTCAGGGCATGGCCAAAGCGTCAGAGACCGA
ACGTTCGGGCCCCGGCACCCAACCGGCGGACGCCCAGACCGCGACGTCCG
CGACGGTTCGACCCCTGAGCACCCAGGCGGTGTTCCGCCCCGATTTCGGC
GATGAGGACAACTTCCCCCATCCGACGCTCGGCCCGGACACCGAGCCGCA
AGACCGGATGGCCACCACCAGCCGGGTGCGCCCGCCGGTCAGACGGCTGG
GCGGCGGCCTGGTGGAAATCCCGCGGGCGCCCGATATCGATCCGCTTGAG
GCCCTGATGACCAACCCGGTGGTGCCGGAGTCCAAGCGGTTCTGCTGGAA
CTGTGGACGTCCCGTCGGCCGGTCCGACTCGGAGACCAAGGGAGCTTCAG
AGGGCTGGTGTCCCTATTGCGGCAGCCCGTATTCGTTCCTGCCGCAGCTA
AATCCCGGGGACATCGTCGCCGGCCAGTACGAGGTCAAAGGCTGCATCGC
GCACGGCGGACTGGGCTGGATCTACCTCGCTCTCGACCGCAATGTCAACG
GCCGTCCGGTGGTGCTCAAGGGCCTGGTGCATTCCGGTGATGCCGAAGCG
CAGGCAATGGCGATGGCCGAACGCCAGTTCCTGGCCGAGGTGGTGCACCC
GTCGATCGTGCAGATCTTCAACTTTGTCGAGCACACCGACAGGCACGGGG
ATCCGGTCGGCTACATCGTGATGGAATACGTCGGCGGGCAATCGCTCAAA
CGCAGCAAGGGTCAGAAACTGCCCGTCGCGGAGGCCATCGCCTACCTGCT
GGAGATCCTGCCGGCGCTGAGCTACCTGCATTCCATCGGCTTGGTCTACA
ACGACCTGAAGCCGGAAAACATCATGCTGACCGAGGAACAGCTCAAGCTG
ATCGACCTGGGCGCGGTATCGCGGATCAACTCGTTCGGCTACCTCTACGG
GACCCCAGGCTTCCAGGCGCCCGAGATCGTGCGGACCGGTCCGACGGTGG
CCACCGACATCTACACCGTGGGACGCACGCTCGCGGCGCTCACGCTGGAC
CTGCCCACCCGCAATGGCCGTTATGTGGATGGGCTACCCGAAGACGACCC
GGTGCTGAAAACCTACGACTCTTACGGCCGGTTGCTGCGCAGGGCCATCG
ACCCCGATCCGCGGCAACGGTTCACCACCGCCGAAGAGATGTCCGCGCAA
TTGACGGGCGTGTTGCGGGAGGTGGTCGCCCAGGACACCGGGGTGCCGCG
GCCAGGGCTATCAACGATCTTCAGTCCCAGTCGGTCGACATTTGGAGTGG
ACCTGCTGGTGGCGCACACCGACGTGTATCTGGACGGGCAGGTGCACGCG
GAGAAGCTGACCGCCAACGAGATCGTGACCGCGCTGTCGGTGCCGCTGGT
CGATCCGACCGACGTCGCAGCTTCGGTCCTGCAGGCCACGGTGCTCTCCC
AGCCGGTGCAGACCCTAGACTCGCTGCGCGCGGCCCGCCACGGTGCGCTG
GACGCCGACGGCGTCGACTTCTCCGAGTCAGTGGAGCTGCCGCTAATGGA
AGTCCGCGCGCTGCTGGATCTCGGCGATGTGGCCAAGGCCACCCGAAAAC
TCGACGATCTGGCCGAACGCGTTGGCTGGCGATGGCGATTGGTCTGGTAC
CGGGCCGTCGCCGAGCTGCTCACCGGCGACTATGACTCGGCCACCAAACA
TTTCACCGAGGTGCTGGATACCTTTCCCGGCGAGCTGGCGCCCAAGCTCG
CCCTGGCCGCCACCGCCGAACTAGCCGGCAACACCGACGAACACAAGTTC
TATCAGACGGTGTGGAGCACCAACGACGGCGTGATCTCGGCGGCTTTCGG
ACTGGCCAGAGCCCGGTCGGCCGAAGGTGATCGGGTCGGCGCCGTGCGCA
CGCTCGACGAGGTACCGCCCACTTCTCGGCATTTCACCACGGCACGGCTG
ACCAGCGCGGTGACTCTGTTGTCCGGCCGGTCAACGAGTGAAGTCACCGA
GGAACAGATCCGCGACGCCGCCCGAAGAGTGGAGGCGCTGCCCCCGACCG
AACCACGCGTGCTGCAGATCCGCGCCCTGGTGCTGGGTGGCGCGCTGGAC
TGGCTGAAGGACAACAAGGCCAGCACCAACCACATCCTCGGTTTCCCGTT
CACCAGTCACGGGCTGCGGCTGGGTGTCGAGGCGTCACTGCGCAGCCTGG
CCCGGGTAGCTCCCACTCAACGGCATCGCTACACGCTGGTGGACATGGCC
AACAAGGTCCGGCCCACCAGCACGTTCTAAGAATTC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
EFENLYFQGMAKASETERSGPGTQPADAQTATSATVRPLSTQAVFRPDFG
DEDNFPHPTLGPDTEPQDRMATTSRVRPPVRRLGGGLVEIPRAPDIDPLE
ALMTNPVVPESKRFCWNCGRPVGRSDSETKGASEGWCPYCGSPYSFLPQL
NPGDIVAGQYEVKGCIAHGGLGWIYLALDRNVNGRPVVLKGLVHSGDAEA
QAMAMAERQFLAEVVHPSIVQIFNFVEHTDRHGDPVGYIVMEYVGGQSLK
RSKGQKLPVAEAIAYLLEILPALSYLHSIGLVYNDLKPENIMLTEEQLKL
IDLGAVSRINSFGYLYGTPGFQAPEIVRTGPTVATDIYTVGRTLAALTLD
LPTRNGRYVDGLPEDDPVLKTYDSYGRLLRRAIDPDPRQRFTTAEEMSAQ
LTGVLREVVAQDTGVPRPGLSTIFSPSRSTFGVDLLVAHTDVYLDGQVHA
EKLTANEIVTALSVPLVDPTDVAASVLQATVLSQPVQTLDSLRAARHGAL
DADGVDFSESVELPLMEVRALLDLGDVAKATRKLDDLAERVGWRWRLVWY
RAVAELLTGDYDSATKHFTEVLDTFPGELAPKLALAATAELAGNTDEHKF
YQTVWSTNDGVISAAFGLARARSAEGDRVGAVRTLDEVPPTSRHFTTARL
TSAVTLLSGRSTSEVTEEQIRDAARRVEALPPTEPRVLQIRALVLGGALD
WLKDNKASTNHILGFPFTSHGLRLGVEASLRSLARVAPTQRHRYTLVDMA
NKVRPTSTF*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU35216
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GFP for mammalian expression; ; Estimated MW of expressed protein : 110762 Daltons