cDNA Clone TNNI3K

• Plasmid: pGEX6P-1-TNNI3K
• Parent Plasmid: pGEX6P1
• Expressed: GST TNNI3K
• Genbank: AAH32865.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCAGCTGCTAGGGACCCTCCGGAAGTATCGCTGCGAGAAGC
CACCCAGCGAAAATTGCGGAGGTTTTCCGAGCTAAGAGGCAAACTTGTAG
CACGTGGAGAATTCTGGGACATAGTTGCAATAACAGCGGCTGATGAAAAA
CAGGAACTTGCTTACAACCAACAGCTGTCAGAAAAGCTGAAAAGAAAGGA
GTTACCCCTTGGAGTTCAATATCACGTTTTTGTAGATCCTGCTGGAGCCA
AAATTGGAAATGGAGGATCAACACTTTGTGCCCTTCAATGTTTGGAAAAG
CTATATGGAGATAAATGGAATTCTTTTACCATCTTATTAATTCACTCTGA
TGAATGGAAGAAAAAAGTCAGTGAATCATATGTTATCACAATAGAAAGAT
TAGAAGATGACCTGCAGATCAAGGAAAAAGAACTGACAGAACTAAGGAAT
ATATTTGGCTCTGATGAAGCCTTCAGTAAAGTCAATTTAAATTACCGCAC
TGAAAATGGGCTGTCTCTACTTCATTTATGTTGCATTTGTGGAGGCAAGA
AATCACATATTCGAACTCTTATGTTGAAAGGGCTCCGCCCATCTCGACTG
ACAAGAAATGGATTTACAGCCTTGCATTTAGCAGTTTACAAGGATAATGC
AGAATTGATCACTTCTCTGCTTCACAGTGGAGCTGATGTACAGCAGGTTG
GATACGGTGGCCTCACTGCCCTCCATATTGCTACAATAGCTGGCCACCTA
GAGGCTGCTGATGTGCTGTTGCAACATGGAGCTAATGTCAATATTCAAGA
TGCAGTTTTTTTCACTCCATTGCATATTGCAGCGTACTATGGACATGAAC
AGGTAACTCGCCTTCTTTTGAAATTTGGTGCTGATGTAAATGTAAGTGGT
GAAGTTGGAGATAGACCCCTCCACCTAGCATCTGCAAAAGGATTCTTGAA
TATTGCAAAACTCTTGATGGAAGAAGGCAGCAAAGCAGATGTGAATGCTC
AAGATAATGAAGACCATGTCCCACTCCATTTCTGTTCTCGATTTGGACAC
CATGATATAGTTAAGTATCTGCTGCAAAGTGATTTGGAAGTTCAACCTCA
TGTTGTTAATATCTATGGAGATACCCCCTTACACCTGGCATGCTACAATG
GCAAATTTGAAGTTGCCAAGGAAATCATCCAAATATCAGGAACAGAAAGT
CTGACTAAGGAAAACATCTTCAGTGAAACAGCTTTTCATAGTGCTTGTAC
CTATGGCAAGAGCATTGACCTAGTCAAATTTCTTCTTGATCAGAATGTCA
TAAACATCAACCACCAAGGAAGGGATGGGCACACTGGATTACACTCTGCT
TGCTACCACGGTCACATTCGCCTGGTTCAGTTCTTACTGGATAATGGAGC
TGATATGAATCTAGTGGCTTGTGATCCCAGCAGGTCTAGTGGTGAAAAAG
ATGAGCAGACATGTTTGATGTGGGCTTATGAAAAAGGGCATGATGCCATT
GTCACACTCCTGAAGCATTATAAGAGACCACAAGATGAATTGCCCTGTAA
TGAATATTCTCAGCCTGGAGGAGATGGCTCCTATGTGTCTGTTCCATCAC
CCTTGGGGAAGATTAAAAGCATGACAAAAGAGAAGGCAGATATTCTCCTC
CTAAGAGCTGGATTGCCTTCACATTTCCATCTTCAGCTCTCAGAAATTGA
GTTCCATGAGATTATTGGCTCAGGTTCTTTTGGGAAAGTATATAAAGGAC
GATGCAGAAATAAAATAGTGGCTATAAAACGTTATCGAGCCAATACCTAC
TGCTCCAAGTCAGATGTGGATATGTTTTGCCGAGAGGTGTCCATTCTCTG
CCAGCTCAATCATCCCTGCGTAATTCAGTTTGTGGGTGCTTGCTTGAATG
ATCCCAGCCAGTTTGCCATTGTCACTCAATACATATCAGGGGGTTCTCTG
TTCTCCCTCCTTCATGAGCAGAAGAGGATTCTTGATTTGCAGTCTAAATT
AATTATTGCAGTAGATGTTGCCAAAGGCATGGAGTACCTTCACAACCTGA
CACAGCCAATTATACATCGTGACTTGAACAGTCACAATATTCTTCTCTAT
GAGGATGGGCATGCTGTGGTGGCAGATTTTGGAGAATCAAGATTTCTACA
GTCTCTGGATGAAGACAACATGACAAAACAACCTGGGAACCTCCTTTGGA
TGGCTCCTGAGGTGTTCACGCAGTGCACTCGGTACACCATCAAAGCAGAT
GTCTTCAGCTATGCTCTGTGTCTGTGGGAAATTCTCACTGGCGAAATTCC
ATTCGCTCATCTCAAGCCAGCGGCTGCGGCAGCAGACATGGCTTACCACC
ACATCAGACCTCCCATTGGCTATTCCATTCCCAAGCCCATATCATCTCTG
CTGATACGAGGGTGGAACGCATGTCCTGAAGCAAAATCCAGACCAAGTCA
TTACCCAGTCTCATCTGTGTACACAGAAACTCTTAAGAAGAAAAATGAAG
ATCGTTTTGGGATGTGGATTGAGTATCTCAGAAGATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAAARDPPEVSLREATQRK
LRRFSELRGKLVARGEFWDIVAITAADEKQELAYNQQLSEKLKRKELPLG
VQYHVFVDPAGAKIGNGGSTLCALQCLEKLYGDKWNSFTILLIHSDEWKK
KVSESYVITIERLEDDLQIKEKELTELRNIFGSDEAFSKVNLNYRTENGL
SLLHLCCICGGKKSHIRTLMLKGLRPSRLTRNGFTALHLAVYKDNAELIT
SLLHSGADVQQVGYGGLTALHIATIAGHLEAADVLLQHGANVNIQDAVFF
TPLHIAAYYGHEQVTRLLLKFGADVNVSGEVGDRPLHLASAKGFLNIAKL
LMEEGSKADVNAQDNEDHVPLHFCSRFGHHDIVKYLLQSDLEVQPHVVNI
YGDTPLHLACYNGKFEVAKEIIQISGTESLTKENIFSETAFHSACTYGKS
IDLVKFLLDQNVININHQGRDGHTGLHSACYHGHIRLVQFLLDNGADMNL
VACDPSRSSGEKDEQTCLMWAYEKGHDAIVTLLKHYKRPQDELPCNEYSQ
PGGDGSYVSVPSPLGKIKSMTKEKADILLLRAGLPSHFHLQLSEIEFHEI
IGSGSFGKVYKGRCRNKIVAIKRYRANTYCSKSDVDMFCREVSILCQLNH
PCVIQFVGACLNDPSQFAIVTQYISGGSLFSLLHEQKRILDLQSKLIIAV
DVAKGMEYLHNLTQPIIHRDLNSHNILLYEDGHAVVADFGESRFLQSLDE
DNMTKQPGNLLWMAPEVFTQCTRYTIKADVFSYALCLWEILTGEIPFAHL
KPAAAAADMAYHHIRPPIGYSIPKPISSLLIRGWNACPEAKSRPSHYPVS
SVYTETLKKKNEDRFGMWIEYLRR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35150
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 121280 Daltons