cDNA Clone PYGM (mouse)

• Plasmid: pET24a PYGM P49T
• Parent Plasmid: pET24a original
• Expressed: PYGM P49T
• Genbank: AAD30476
• Species: Mouse

Sequence of Insert

ACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTGCGCGGCCGCGAT
GTCCAGGCCTCTTTCAGACCAGGATAAGAGAAAGCAAATCAGCGTTCGTG
GCTTAGCCGGAGTGGAAAATGTGTCGGAGCTAAAAAAGAACTTCAACCGC
CACCTGCATTTCACCTTGGTCAAGGATCGCAATGTGGCTACTACGAGAGA
TTACTATTTTGCACTGGCCCACACTGTCCGGGACCACCTCGTGGGACGCT
GGATCCGCACACAGCAGCATTACTACGAAAAGGACCCCAAGAGGATCTAC
TACCTGTCTTTGGAGTTCTACATGGGCCGGACACTGCAGAACACCATGGT
GAACCTGGCCTTGGAGAACGCCTGTGATGAGGCCACTTACCAGCTGGGCT
TGGACATGGAGGAGTTGGAGGAGATCGAGGAGGATGCAGGGTTGGGCAAT
GGGGGCCTGGGACGCCTGGCAGCTTGCTTTTTGGACTCCATGGCTACCCT
GGGGCTCGCTGCCTATGGCTATGGGATCCGCTATGAGTTTGGGATTTTTA
ATCAGAAGATCTGTGGGGGCTGGCAGATGGAAGAAGCTGATGACTGGCTT
CGCTATGGCAACCCCTGGGAGAAGGCCCGCCCAGAGTTCACGCTGCCTGT
GCATTTCTATGGCCGAGTGGAGCACACCAGCCAGGGTGCCAAGTGGGTGG
ACACACAGGTAGTGCTGGCTATGCCCTATGACACTCCTGTGCCCGGCTAT
CGGAACAACGTCGTCAACACCATGCGCCTCTGGTCGGCCAAGGCACCTAA
CGACTTCAACCTCAAGGACTTCAATGTTGGCGGCTACATCCAGGCTGTGC
TGGACCGAAACCTGGCTGAGAACATCTCGCGTGTTTTGTACCCCAATGAT
AATTTCTTTGAAGGGAAGGAGCTGCGGCTGAAGCAGGAGTACTTTGTGGT
GGCTGCCACCCTCCAGGACATCATCCGGCGTTTCAAGTCCTCCAAGTTTG
GCAGCCGTGATCCTGTGCGCACAAACTTCGATGCCTTCCCTGATAAGGTG
GCCATCCAGCTCAATGACACCCACCCCTCTTTGGCCATCCCAGAGCTGAT
GAGGATTCTGGTGGACCTGGAACGACTGGACTGGGACAAGGCCTGGGACG
TAACAGTGAAGACCTGTGCCTACACCAACCACACGGTGCTGCCTGAAGCC
CTGGAGCGCTGGCCAGTGCACCTCATGGAGACACTGCTGCCCCGCCACTT
GCAGATCATTTACGAGATCAACCAGCGTTTCCTTAACCGGGTGGCGGCTG
CATTCCCTGGGGACGTAGATAGGCTGCGGCGCATGTCACTCGTGGAGGAA
GGAGCAGTGAAACGCATCAACATGGCACACCTGTGCATTGCTGGCTCGCA
CGCTGTCAACGGCGTGGCTCGCATCCATTCGGAGATCCTCAAGAAAACCA
TCTTCAAGGACTTTTATGAGCTGGAGCCTCACAAGTTCCAGAACAAGACC
AATGGCATCACTCCTCGGCGCTGGCTGGTTCTGTGTAACCCTGGGCTGGC
AGAGGTCATTGCTGAGCGCATTGGTGAGGACTACATCTCAGACCTGGACC
AGCTGCGCAAGCTGCTCTCCTATGTGGATGATGAAGCCTTTATCCGTGAT
GTGGCCAAAGTGAAACAGGAAAACAAGTTGAAGTTCTCTGCATACCTCGA
GAGGGAATACAAAGTCCACATCAACCCCAACTCGCTCTTCGACGTCCAGG
TGAAAAGGATTCATGAGTACAAGCGGCAGCTCCTCAACTGCCTGCACATC
ATCACCTTGTATAACCGCATCAAAAGGGAGCCCAATAGGTTTATGGTGCC
GAGGACTATCATGATTGGAGGCAAGGCTGCACCCGGGTACCACATGGCTA
AGATGATCATCAAGCTTATCACTGCCATTGGGGATGTGGTCAACCACGAC
CCTGCGGTAGGAGACCGCCTCCGAGTGATCTTCCTGGAGAACTACCGAGT
CTCCCTTGCTGAGAAAGTTATTCCTGCCGCCGACCTCTCAGAGCAGATCT
CCACCGCGGGCACTGAGGCCTCGGGCACTGGCAACATGAAGTTCATGCTC
AATGGGGCTCTGACCATCGGCACCATGGACGGTGCCAACGTGGAGATGGC
AGAGGAGGCGGGGGAGGAGAACTTCTTCATCTTCGGCATGCGAGTGGAGG
ACGTGGAAAGGCTGGACCAGAGAGGGTACAATGCCCAGGAGTACTACGAC
CGAATTCCTGAGCTTCGGCAGATCATCGAACAGCTAAGCAGCGGCTTCTT
CTCCCCCAAGCAGCCTGACCTGTTCAAGGACATTGTCAATATGCTCATGC
ACCATGACCGGTTTAAAGTCTTTGCAGATTATGAGGAATACATTAAATGC
CAGGACAAAGTCAGTGAGTTGTACAAGAACCCAAGAGAGTGGACACGGAT
GGTGATCAGGAACATAGCCACCTCTGGCAAGTTTTCCAGTGACCGCACCA
TTGCCCAGTATGCCCGGGAGATCTGGGGTGTTGAACCTTCTCGCCAGCGC
CTGCCAGCCCCAGATGAGAAGATCTGAGCGGCCGCGCAAGGGCGAATTCT
GCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGA

Amino Acid Sequence

MSRPLSDQDKRKQISVRGLAGVENVSELKKNFNRHLHFTLVKDRNVATTR
DYYFALAHTVRDHLVGRWIRTQQHYYEKDPKRIYYLSLEFYMGRTLQNTM
VNLALENACDEATYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMAT
LGLAAYGYGIRYEFGIFNQKICGGWQMEEADDWLRYGNPWEKARPEFTLP
VHFYGRVEHTSQGAKWVDTQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAP
NDFNLKDFNVGGYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFV
VAATLQDIIRRFKSSKFGSRDPVRTNFDAFPDKVAIQLNDTHPSLAIPEL
MRILVDLERLDWDKAWDVTVKTCAYTNHTVLPEALERWPVHLMETLLPRH
LQIIYEINQRFLNRVAAAFPGDVDRLRRMSLVEEGAVKRINMAHLCIAGS
HAVNGVARIHSEILKKTIFKDFYELEPHKFQNKTNGITPRRWLVLCNPGL
AEVIAERIGEDYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFSAYL
EREYKVHINPNSLFDVQVKRIHEYKRQLLNCLHIITLYNRIKREPNRFMV
PRTIMIGGKAAPGYHMAKMIIKLITAIGDVVNHDPAVGDRLRVIFLENYR
VSLAEKVIPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEM
AEEAGEENFFIFGMRVEDVERLDQRGYNAQEYYDRIPELRQIIEQLSSGF
FSPKQPDLFKDIVNMLMHHDRFKVFADYEEYIKCQDKVSELYKNPREWTR
MVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAPDEKI*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU32929
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Untagged for bacterial expression; ; Estimated MW of expressed protein : 97286 Daltons