cDNA Clone PYGM (mouse)

• Plasmid: pCMV-FLAG-2 PYGM P49G
• Parent Plasmid: pCMV5 FLAG2
• Expressed: FLAG PYGM P49G
• Genbank: AAD30476
• Tag: FLAG
• Species: Mouse

Sequence of Insert

GCGGCCGCGATGTCCAGGCCTCTTTCAGACCAGGATAAGAGAAAGCAAAT
CAGCGTTCGTGGCTTAGCCGGAGTGGAAAATGTGTCGGAGCTAAAAAAGA
ACTTCAACCGCCACCTGCATTTCACCTTGGTCAAGGATCGCAATGTGGCT
ACTGGGAGAGATTACTATTTTGCACTGGCCCACACTGTCCGGGACCACCT
CGTGGGACGCTGGATCCGCACACAGCAGCATTACTACGAAAAGGACCCCA
AGAGGATCTACTACCTGTCTTTGGAGTTCTACATGGGCCGGACACTGCAG
AACACCATGGTGAACCTGGCCTTGGAGAACGCCTGTGATGAGGCCACTTA
CCAGCTGGGCTTGGACATGGAGGAGTTGGAGGAGATCGAGGAGGATGCAG
GGTTGGGCAATGGGGGCCTGGGACGCCTGGCAGCTTGCTTTTTGGACTCC
ATGGCTACCCTGGGGCTCGCTGCCTATGGCTATGGGATCCGCTATGAGTT
TGGGATTTTTAATCAGAAGATCTGTGGGGGCTGGCAGATGGAAGAAGCTG
ATGACTGGCTTCGCTATGGCAACCCCTGGGAGAAGGCCCGCCCAGAGTTC
ACGCTGCCTGTGCATTTCTATGGCCGAGTGGAGCACACCAGCCAGGGTGC
CAAGTGGGTGGACACACAGGTAGTGCTGGCTATGCCCTATGACACTCCTG
TGCCCGGCTATCGGAACAACGTCGTCAACACCATGCGCCTCTGGTCGGCC
AAGGCACCTAACGACTTCAACCTCAAGGACTTCAATGTTGGCGGCTACAT
CCAGGCTGTGCTGGACCGAAACCTGGCTGAGAACATCTCGCGTGTTTTGT
ACCCCAATGATAATTTCTTTGAAGGGAAGGAGCTGCGGCTGAAGCAGGAG
TACTTTGTGGTGGCTGCCACCCTCCAGGACATCATCCGGCGTTTCAAGTC
CTCCAAGTTTGGCAGCCGTGATCCTGTGCGCACAAACTTCGATGCCTTCC
CTGATAAGGTGGCCATCCAGCTCAATGACACCCACCCCTCTTTGGCCATC
CCAGAGCTGATGAGGATTCTGGTGGACCTGGAACGACTGGACTGGGACAA
GGCCTGGGACGTAACAGTGAAGACCTGTGCCTACACCAACCACACGGTGC
TGCCTGAAGCCCTGGAGCGCTGGCCAGTGCACCTCATGGAGACACTGCTG
CCCCGCCACTTGCAGATCATTTACGAGATCAACCAGCGTTTCCTTAACCG
GGTGGCGGCTGCATTCCCTGGGGACGTAGATAGGCTGCGGCGCATGTCAC
TCGTGGAGGAAGGAGCAGTGAAACGCATCAACATGGCACACCTGTGCATT
GCTGGCTCGCACGCTGTCAACGGCGTGGCTCGCATCCATTCGGAGATCCT
CAAGAAAACCATCTTCAAGGACTTTTATGAGCTGGAGCCTCACAAGTTCC
AGAACAAGACCAATGGCATCACTCCTCGGCGCTGGCTGGTTCTGTGTAAC
CCTGGGCTGGCAGAGGTCATTGCTGAGCGCATTGGTGAGGACTACATCTC
AGACCTGGACCAGCTGCGCAAGCTGCTCTCCTATGTGGATGATGAAGCCT
TTATCCGTGATGTGGCCAAAGTGAAACAGGAAAACAAGTTGAAGTTCTCT
GCATACCTCGAGAGGGAATACAAAGTCCACATCAACCCCAACTCGCTCTT
CGACGTCCAGGTGAAAAGGATTCATGAGTACAAGCGGCAGCTCCTCAACT
GCCTGCACATCATCACCTTGTATAACCGCATCAAAAGGGAGCCCAATAGG
TTTATGGTGCCGAGGACTATCATGATTGGAGGCAAGGCTGCACCCGGGTA
CCACATGGCTAAGATGATCATCAAGCTTATCACTGCCATTGGGGATGTGG
TCAACCACGACCCTGCGGTAGGAGACCGCCTCCGAGTGATCTTCCTGGAG
AACTACCGAGTCTCCCTTGCTGAGAAAGTTATTCCTGCCGCCGACCTCTC
AGAGCAGATCTCCACCGCGGGCACTGAGGCCTCGGGCACTGGCAACATGA
AGTTCATGCTCAATGGGGCTCTGACCATCGGCACCATGGACGGTGCCAAC
GTGGAGATGGCAGAGGAGGCGGGGGAGGAGAACTTCTTCATCTTCGGCAT
GCGAGTGGAGGACGTGGAAAGGCTGGACCAGAGAGGGTACAATGCCCAGG
AGTACTACGACCGAATTCCTGAGCTTCGGCAGATCATCGAACAGCTAAGC
AGCGGCTTCTTCTCCCCCAAGCAGCCTGACCTGTTCAAGGACATTGTCAA
TATGCTCATGCACCATGACCGGTTTAAAGTCTTTGCAGATTATGAGGAAT
ACATTAAATGCCAGGACAAAGTCAGTGAGTTGTACAAGAACCCAAGAGAG
TGGACACGGATGGTGATCAGGAACATAGCCACCTCTGGCAAGTTTTCCAG
TGACCGCACCATTGCCCAGTATGCCCGGGAGATCTGGGGTGTTGAACCTT
CTCGCCAGCGCCTGCCAGCCCCAGATGAGAAGATCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMSRPLSDQDKRKQISVRGLAGVENVSEL
KKNFNRHLHFTLVKDRNVATGRDYYFALAHTVRDHLVGRWIRTQQHYYEK
DPKRIYYLSLEFYMGRTLQNTMVNLALENACDEATYQLGLDMEELEEIEE
DAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKICGGWQME
EADDWLRYGNPWEKARPEFTLPVHFYGRVEHTSQGAKWVDTQVVLAMPYD
TPVPGYRNNVVNTMRLWSAKAPNDFNLKDFNVGGYIQAVLDRNLAENISR
VLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGSRDPVRTNFD
AFPDKVAIQLNDTHPSLAIPELMRILVDLERLDWDKAWDVTVKTCAYTNH
TVLPEALERWPVHLMETLLPRHLQIIYEINQRFLNRVAAAFPGDVDRLRR
MSLVEEGAVKRINMAHLCIAGSHAVNGVARIHSEILKKTIFKDFYELEPH
KFQNKTNGITPRRWLVLCNPGLAEVIAERIGEDYISDLDQLRKLLSYVDD
EAFIRDVAKVKQENKLKFSAYLEREYKVHINPNSLFDVQVKRIHEYKRQL
LNCLHIITLYNRIKREPNRFMVPRTIMIGGKAAPGYHMAKMIIKLITAIG
DVVNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTG
NMKFMLNGALTIGTMDGANVEMAEEAGEENFFIFGMRVEDVERLDQRGYN
AQEYYDRIPELRQIIEQLSSGFFSPKQPDLFKDIVNMLMHHDRFKVFADY
EEYIKCQDKVSELYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGV
EPSRQRLPAPDEKI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32919
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression; ; Estimated MW of expressed protein : 99518 Daltons