cDNA Clone SENP2

• Plasmid: pFBDual 6P-1 SENP2
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST SENP2
• Genbank: AK304402.1
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGTACAGATGGCTGGTTAGGATTCTCGGCACCATTTTCCGTTT
CTGCGACCGGTCGGTGCCCCCTGCCCGGGCCCTCCTGAAGAGGCGGCGCT
CAGACAGCACTCTGTTTTCTACAGTGGACACTGATGAAATACCAGCCAAA
AGACCAAGATTAGATTGCTTTATTCACCAAGTGAAAAACAGTCTCTACAA
TGCTGCCAGCTTATTTGGATTCCCATTCCAGCTGACCACAAAGCCCATGG
TAACTTCTGCTTGTAATGGAACACGGAATGTGGCCCCTTCAGGAGAGGTA
TTTTCGAACTCTTCATCTTGTGAACTGACAGGTTCTGGATCCTGGAACAA
CATGCTGAAACTGGGTAATAAATCTCCTAATGGAATAAGTGACTATCCAA
AGATCAGAGTGACAGTTACCCGAGATCAGCCACGCAGAGTCCTGCCTTCC
TTTGGTTTTACTTTGAACTCAGAAGGCTGTAATAGAAGACCAGGTGGCCG
TCGCCATAGCAAAGGTAATCCAGAGAGTTCTTTAATGTGGAAACCTCGGG
AACAGGCTGTAACAGAGATGATTTCTGAAGAGAGTGGCAAGGGTCTGAGG
CGTCCCCATTGTACTGTGGAGGAGGGTGTTCAAAAAGAGGAAAGAGAGAA
GTACCGAAAGTTATTGGAACGACTTAAAGAAAGTGGTCATGGAAACTCTG
TCTGTCCTGTAACTTCAAATTATCACAGTTCTCAAAGAAGTCAGATGGAC
ACATTAAAGACCAAAGGCTGGGGGGAAGAGCAAAATCACGGAGTCAAAAC
AACTCAGTTTGTTCCAAAACAATATAGACTTGTTGAAACAAGGGGACCTC
TATGTTCATTGAGAAGTGAAAAGAGGTGTTCAAAGGGGAAAATTACTGAT
ACAGAGACGATGGTCGGAATCAGATTTGAAAATGAAAGTAGGAGGGGATA
CCAACTGGAGCCTGACCTATCAGAAGAAGTGTCGGCCCGACTCCGCCTGG
GCAGTGGAAGCAATGGCTTACTCAGGAGGAAAGTGTCAATAATTGAGACA
AAGGAAAAGAATTGCTCAGGCAAAGAGAGGGACAGAAGAACGGACGATCT
CCTTGAACTTACAGAGGACATGGAAAAGGAAATCAGTAATGCCCTAGGCC
ATGGCCCACAGGATGAAATCCTAAGTAGTGCTTTCAAATTGCGAATTACT
CGAGGAGATATTCAGACATTAAAGAACTATCACTGGCTCAATGATGAAGT
CATTAATTTTTACATGAATCTTCTGGTGGAAAGAAATAAAAAGCAAGGCT
ATCCAGCACTTCATGTATTCAGTACTTTCTTCTATCCTAAATTAAAGTCT
AGGGGTTACCAAGCAGTGAAACGATGGACCAAAGGGGTAAATCTCTTTGA
ACAAGAAATTATTCTGGTGCCTATTCATCGGAAGGTACATTGGAGCCTGG
TGGTGATTGACCTAAGAAAAAAGTGTCTTAAATATCTGGATTCTATGGGA
CAAAAGGGCCACAGGATCTGTGAGATTCTCCTTCAGTATTTACAGGATGA
AAGTAAGACCAAAAGAAATAGTGATCTGAATCTTTTAGAGTGGACCCATC
ACAGCATGAAACCACACGAGATTCCTCAACAGCTGAATGGGAGTGATTGT
GGAATGTTTACTTGTAAATATGCAGATTATATTTCTAGGGACAAACCTAT
CACATTTACTCAGCACCAGATGCCTCTCTTCCGGAAGAAGATGGTGTGGG
AAATCCTTCATCAGCAGTTGCTGTGAGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMYRWLVRILGTIFRFC
DRSVPPARALLKRRRSDSTLFSTVDTDEIPAKRPRLDCFIHQVKNSLYNA
ASLFGFPFQLTTKPMVTSACNGTRNVAPSGEVFSNSSSCELTGSGSWNNM
LKLGNKSPNGISDYPKIRVTVTRDQPRRVLPSFGFTLNSEGCNRRPGGRR
HSKGNPESSLMWKPREQAVTEMISEESGKGLRRPHCTVEEGVQKEEREKY
RKLLERLKESGHGNSVCPVTSNYHSSQRSQMDTLKTKGWGEEQNHGVKTT
QFVPKQYRLVETRGPLCSLRSEKRCSKGKITDTETMVGIRFENESRRGYQ
LEPDLSEEVSARLRLGSGSNGLLRRKVSIIETKEKNCSGKERDRRTDDLL
ELTEDMEKEISNALGHGPQDEILSSAFKLRITRGDIQTLKNYHWLNDEVI
NFYMNLLVERNKKQGYPALHVFSTFFYPKLKSRGYQAVKRWTKGVNLFEQ
EIILVPIHRKVHWSLVVIDLRKKCLKYLDSMGQKGHRICEILLQYLQDES
KTKRNSDLNLLEWTHHSMKPHEIPQQLNGSDCGMFTCKYADYISRDKPIT
FTQHQMPLFRKKMVWEILHQQLL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32815
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for baculovirus expression. Sequence agrees with genbank except for G449R change which occurs in the original Hay construct.; ; Estimated MW of expressed protein : 95178 Daltons