cDNA Clone SENP1

• Plasmid: pFBHTa SENP1
• Parent Plasmid: pSC-A
• Expressed: 6His SENP1
• Genbank: AK292060.1
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCATGGATGATATTGCTGATAGGATGAGGATGGATGCTGGAGAAGT
GACTTTAGTGAACCACAACTCCGTATTCAAAACCCACCTCCTGCCACAAA
CAGGTTTTCCAGAGGACCAGCTTTCGCTTTCTGACCAGCAGATTTTATCT
TCCAGGCAAGGACATTTGGACCGATCTTTTACATGTTCCACAAGAAGTGC
AGCTTATAATCCAAGCTATTACTCAGATAATCCTTCCTCAGACAGTTTTC
TTGGCTCAGGCGATTTAAGAACCTTTGGCCAGAGTGCAAATGGCCAATGG
AGAAATTCTACCCCATCGTCAAGCTCATCTTTACAAAAATCAAGAAACAG
CCGAAGTCTTTACCTCGAAACCCGAAAGACCTCAAGTGGATTATCAAACA
GTTTTGCGGGAAAGTCAAACCATCACTGCCATGTATCTGCATATGAAAAA
TCTTTTCCTATTAAACCTGTTCCAAGTCCATCTTGGAGTGGTTCATGTCG
TCGAAGTCTCTTGAGCCCCAAGAAAACTCAGAGGCGACATGTTAGTACAG
CAGAAGAGACAGTTCAAGAAGAAGAAAGAGAGATTTACAGACAGCTGCTA
CAGATGGTCACAGGGAAACAGTTTACTATAGCCAAACCCACCACACATTT
TCCTTTACACCTGTCTCGATGTCTTAGTTCCAGTAAAAATACTTTGAAAG
ACTCACTGTTTAAAAATGGAAACTCTTGTGCATCTCAGATCATTGGCTCT
GATACTTCATCATCTGGATCTGCCAGCATTTTAACTAACCAGGAACAGCT
GTCCCACAGTGTATATTCCCTATCTTCTTATACCCCAGATGTTGCATTTG
GATCCAAAGATTCTGGTACTCTTCATCATCCCCATCATCACCACTCTGTT
CCACATCAGCCAGATAACTTAGCAGCTTCAAATACACAATCTGAAGGATC
AGACTCTGTGATTTTACTGAAAGTGAAAGATTCCCAGACTCCAACTCCCA
GTTCTACTTTCTTCCAGGCAGAGCTGTGGATCAAAGAATTAACTAGTGTT
TATGATTCTCGAGCACGAGAAAGATTGCGCCAGATTGACGAACAGAAGGC
ATTGGCCTTACAGCTTCAAAACCAGAGATTGCAGGAGCGGGAACATTCAG
TACATGATTCAGTAGAACTACATCTTCGTGTACCTCTTGAAAAGGAGATT
CCTGTTACTGTTGTCCAAGAAACACAAAAAAAAGGTCATAAATTAACTGA
TAGTGAAGATGAATTTCCTGAAATTACAGAGGAAATGGAGAAAGAAATAA
AGAATGTATTTCGTAATGGGAATCAGGATGAAGTTCTCAGTGAAGCATTT
CGCCTGACCATTACACGCAAAGATATTCAAACTCTAAACCATCTGAATTG
GCTCAATGATGAGATCATCAATTTCTACATGAATATGCTGATGGAGCGAA
GTAAAGAGAAGGGCTTGCCAAGTGTGCATGCATTTAATACCTTTTTCTTC
ACTAAATTAAAAACGGCTGGTTATCAGGCAGTGAAACGTTGGACAAAGAA
AGTAGATGTATTTTCTGTTGACATTCTTTTGGTGCCCATTCACCTGGGAG
TACACTGGTGTCTAGCTGTTGTGGACTTTAGAAAGAAGAATATTACCTAT
TACGACTCCATGGGTGGGATAAACAATGAAGCCTGCAGAATACTCTTGCA
ATACCTAAAGCAAGAAAGCATTGACAAGAAAAGGAAAGAGTTTGACACCA
ATGGCTGGCAGCTTTTCAGCAAGAAAAGCCAGGAGATTCCTCAGCAGATG
AATGGAAGTGACTGTGGGATGTTTGCCTGCAAATATGCTGACTGTATTAC
CAAAGACAGACCAATCAACTTCACACAGCAACACATGCCATACTTCCGGA
AGCGGATGGTCTGGGAGATCCTCCACCGAAAACTCTTGTGAGTCGAC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMDDIADRMRMDAGEVTLVNH
NSVFKTHLLPQTGFPEDQLSLSDQQILSSRQGHLDRSFTCSTRSAAYNPS
YYSDNPSSDSFLGSGDLRTFGQSANGQWRNSTPSSSSSLQKSRNSRSLYL
ETRKTSSGLSNSFAGKSNHHCHVSAYEKSFPIKPVPSPSWSGSCRRSLLS
PKKTQRRHVSTAEETVQEEEREIYRQLLQMVTGKQFTIAKPTTHFPLHLS
RCLSSSKNTLKDSLFKNGNSCASQIIGSDTSSSGSASILTNQEQLSHSVY
SLSSYTPDVAFGSKDSGTLHHPHHHHSVPHQPDNLAASNTQSEGSDSVIL
LKVKDSQTPTPSSTFFQAELWIKELTSVYDSRARERLRQIDEQKALALQL
QNQRLQEREHSVHDSVELHLRVPLEKEIPVTVVQETQKKGHKLTDSEDEF
PEITEEMEKEIKNVFRNGNQDEVLSEAFRLTITRKDIQTLNHLNWLNDEI
INFYMNMLMERSKEKGLPSVHAFNTFFFTKLKTAGYQAVKRWTKKVDVFS
VDILLVPIHLGVHWCLAVVDFRKKNITYYDSMGGINNEACRILLQYLKQE
SIDKKRKEFDTNGWQLFSKKSQEIPQQMNGSDCGMFACKYADCITKDRPI
NFTQQHMPYFRKRMVWEILHRKLL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32813
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for insect expression. Sequence agrees with genbank except for E361D change which occurs in the original Hay construct.; ; Estimated MW of expressed protein : 77181 Daltons