cDNA Clone SIK

• Plasmid: pEBG6P-SIK
• Parent Plasmid: pEBG6P
• Expressed: GST SIK
• Genbank: NM_173354
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGTTATCATGTCGGAGTTCAGCGCGGACCCCGCGGGCCAGGG
TCAGGGCCAGCAGAAGCCCCTCCGGGTGGGTTTTTACGACATCGAGCGGA
CCCTGGGCAAAGGCAACTTCGCGGTGGTGAAGCTGGCGCGGCATCGAGTC
ACCAAAACGCAGGTTGCAATAAAAATAATTGATAAAACACGATTAGATTC
AAGCAATTTGGAGAAAATCTATCGTGAGGTTCAGCTGATGAAGCTTCTGA
ACCATCCACACATCATAAAGCTTTACCAGGTTATGGAAACAAAGGACATG
CTTTACATCGTCACTGAATTTGCTAAAAATGGAGAAATGTTTGATTATTT
GACTTCCAACGGGCACCTGAGTGAGAACGAGGCGCGGAAGAAGTTCTGGC
AAATCCTGTCGGCCGTGGAGTACTGTCACGACCATCACATCGTCCACCGG
GACCTCAAGACCGAGAACCTCCTGCTGGATGGCAACATGGACATCAAGCT
GGCAGATTTTGGATTTGGGAATTTCTACAAGTCAGGAGAGCCTCTGTCCA
CGTGGTGTGGGAGCCCCCCGTATGCCGCCCCGGAAGTCTTTGAGGGGAAG
GAGTATGAAGGCCCCCAGCTGGACATCTGGAGCCTGGGCGTGGTGCTGTA
CGTCCTGGTCTGCGGTTCTCTCCCCTTCGATGGGCCTAACCTGCCGACGC
TGAGACAGCGGGTGCTGGAGGGCCGCTTCCGCATCCCCTTCTTCATGTCT
CAAGACTGTGAGAGCCTGATCCGCCGCATGCTGGTGGTGGACCCCGCCAG
GCGCATCACCATCGCCCAGATCCGGCAGCACCGGTGGATGCGGGCTGAGC
CCTGCTTGCCGGGACCCGCCTGCCCCGCCTTCTCCGCACACAGCTACACC
TCCAACCTGGGCGACTACGATGAGCAGGCGCTGGGTATCATGCAGACCCT
GGGCGTGGACCGGCAGAGGACGGTGGAGTCACTGCAAAACAGCAGCTATA
ACCACTTTGCTGCCATTTATTACCTCCTCCTTGAGCGGCTCAAGGAGTAT
CGGAATGCCCAGTGCGCCCGCCCCGGGCCTGCCAGGCAGCCGCGGCCTCG
GAGCTCGGACCTCAGTGGTTTGGAGGTGCCTCAGGAAGGTCTTTCCACCG
ACCCTTTCCGACCTGCCTTGCTGTGCCCGCAGCCGCAGACCTTGGTGCAG
TCCGTCCTCCAGGCCGAGATGGACTGTGAGCTCCAGAGCTCGCTGCAGTG
GCCCTTGTTCTTCCCGGTGGATGCCAGCTGCAGCGGAGTGTTCCGGCCCC
GGCCCGTGTCCCCAAGCAGCCTGCTGGACACAGCCATCAGTGAGGAGGCC
AGGCAGGGGCCGGGCCTAGAGGAGGAGCAGGACACGCAGGAGTCCCTGCC
CAGCAGCACGGGCCGGAGGCACACCCTGGCCGAGGTCTCCACCCGCCTCT
CCCCACTCACCGCGCCATGTATAGTCGTCTCCCCCTCCACCACGGCAAGT
CCTGCAGAGGGAACCAGCTCTGACAGTTGTCTGACCTTCTCTGCGAGCAA
AAGCCCCGCGGGGCTCAGTGGCACCCCGGCCACTCAGGGGCTGCTGGGCG
CCTGCTCCCCGGTCAGGCTGGCCTCGCCCTTCCTGGGGTCGCAGTCCGCC
ACCCCAGTGCTGCAGGCTCAGGGGGGCTTGGGAGGAGCTGTTCTGCTCCC
TGTCAGCTTCCAGGAGGGACGGCGGGCGTCGGACACCTCACTGACTCAAG
GGCTGAAGGCCTTTCGGCAGCAGCTGAGGAAGACCACGCGGACCAAAGGG
TTTCTGGGACTGAACAAAATCAAGGGGCTGGCTCGCCAGGTGTGCCAGGC
CCCCGCCAGCCGGGCCAGCAGGGGCGGCCTGAGCCCCTTCCACGCCCCTG
CACAGAGCCCAGGCCTGCACGGCGGCGCAGCCGGCAGCCGGGAGGGCTGG
AGCCTGCTGGAGGAGGTGCTAGAGCAGCAGAGGCTGCTCCAGTTACAGCA
CCACCCGGCCGCTGCACCCGGCTGCTCCCAGGCCCCCCAGCCGGCCCCTG
CCCCGTTTGTGATCGCCCCCTGTGATGGCCCTGGGGCTGCCCCGCTCCCC
AGCACCCTCCTCACGTCGGGGCTCCCGCTGCTGCCGCCCCCACTCCTGCA
GACCGGCGCGTCCCCGGTGGCCTCAGCGGCGCAGCTCCTGGACACACACC
TGCACATTGGCACCGGCCCCACCGCCCTCCCCGCTGTGCCCCCACCACGC
CTGGCCAGGCTGGCCCCAGGTTGTGAGCCCCTGGGGCTGCTGCAGGGGGA
CTGTGAGATGGAGGACCTGATGCCCTGCTCCCTAGGCACGTTTGTCCTGG
TGCAGTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMVIMSEFSADPAG
QGQGQQKPLRVGFYDIERTLGKGNFAVVKLARHRVTKTQVAIKIIDKTRL
DSSNLEKIYREVQLMKLLNHPHIIKLYQVMETKDMLYIVTEFAKNGEMFD
YLTSNGHLSENEARKKFWQILSAVEYCHDHHIVHRDLKTENLLLDGNMDI
KLADFGFGNFYKSGEPLSTWCGSPPYAAPEVFEGKEYEGPQLDIWSLGVV
LYVLVCGSLPFDGPNLPTLRQRVLEGRFRIPFFMSQDCESLIRRMLVVDP
ARRITIAQIRQHRWMRAEPCLPGPACPAFSAHSYTSNLGDYDEQALGIMQ
TLGVDRQRTVESLQNSSYNHFAAIYYLLLERLKEYRNAQCARPGPARQPR
PRSSDLSGLEVPQEGLSTDPFRPALLCPQPQTLVQSVLQAEMDCELQSSL
QWPLFFPVDASCSGVFRPRPVSPSSLLDTAISEEARQGPGLEEEQDTQES
LPSSTGRRHTLAEVSTRLSPLTAPCIVVSPSTTASPAEGTSSDSCLTFSA
SKSPAGLSGTPATQGLLGACSPVRLASPFLGSQSATPVLQAQGGLGGAVL
LPVSFQEGRRASDTSLTQGLKAFRQQLRKTTRTKGFLGLNKIKGLARQVC
QAPASRASRGGLSPFHAPAQSPGLHGGAAGSREGWSLLEEVLEQQRLLQL
QHHPAAAPGCSQAPQPAPAPFVIAPCDGPGAAPLPSTLLTSGLPLLPPPL
LQTGASPVASAAQLLDTHLHIGTGPTALPAVPPPRLARLAPGCEPLGLLQ
GDCEMEDLMPCSLGTFVLVQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32734
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; ; Estimated MW of expressed protein : 112315 Daltons