cDNA Clone PIP5K1A

• Plasmid: pGEX6P-1-PIP5K1A
• Parent Plasmid: pGEX6P1
• Expressed: GST PIP5K1A
• Genbank: NP_003548.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGTCGGCCTCCTCCGGGCCGTCGTCTTCGGTCGGTTTTTC
ATCCTTTGATCCCGCGGTCCCTTCCTGTACCTTGTCCTCAGCATCTGGAA
TCAAGAGACCCATGGCATCTGAGGTGCCTTATGCCTCTGGCATGCCCATC
AAGAAAATAGGCCATAGAAGTGTTGATTCCTCAGGAGAGACAACATATAA
AAAGACAACCTCATCAGCCTTGAAAGGTGCCATCCAGTTAGGCATTACCC
ACACTGTGGGGAGCCTGAGTACCAAACCAGAGCGTGATGTCCTCATGCAA
GATTTCTACGTGGTTGAGAGTATCTTCTTTCCCAGTGAAGGGAGCAACCT
GACCCCTGCTCATCACTACAATGACTTTCGTTTCAAGACCTATGCACCTG
TTGCCTTCCGCTACTTCCGGGAGCTATTTGGTATCCGGCCCGATGATTAC
TTGTATTCCCTCTGCAGTGAGCCGCTGATTGAACTCTGTAGCTCTGGAGC
TAGTGGTTCCCTATTCTATGTGTCCAGCGACGATGAGTTCATTATTAAGA
CAGTCCAACATAAAGAGGCGGAATTTCTGCAGAAGCTGCTTCCAGGATAC
TACATGAACCTCAACCAGAACCCTCGGACTTTGCTGCCTAAATTCTATGG
ACTGTACTGTGTGCAGGCAGGTGGCAAGAACATTCGGATTGTGGTGATGA
ACAATCTTTTACCAAGATCGGTAAAAATGCATATCAAATATGACCTCAAA
GGCTCAACCTACAAACGGCGGGCTTCCCAGAAAGAGCGAGAGAAGCCTCT
TCCCACATTTAAAGACCTAGACTTCTTACAAGACATCCCTGATGGTCTTT
TTTTGGATGCTGACATGTACAACGCTCTCTGTAAGACCCTGCAGCGTGAC
TGTTTGGTGCTGCAGAGCTTCAAGATAATGGATTACAGCCTCTTGATGTC
AATCCATAATATAGATCATGCACAACGAGAGCCCTTAAGCAGTGAAACAC
AGTACTCAGTTGATACTCGAAGACCGGCCCCCCAAAAGGCTCTGTATTCC
ACAGCCATGGAATCCATCCAGGGAGAGGCTCGACGGGGTGGTACCATGGA
GACTGATGACCATATGGGTGGCATCCCTGCCCGGAATAGTAAAGGGGAAA
GGCTTCTGCTTTATATTGGCATCATTGACATTCTACAGTCTTACAGGTTT
GTTAAGAAGTTGGAGCACTCCTGGAAAGCCCTGGTACATGACGGAGACAC
TGTCTCAGTGCATCGCCCAGGCTTCTACGCTGAACGGTTCCAGCGCTTCA
TGTGCAACACAGTATTTAAGAAGATTCCCTTGAAGCCTTCTCCTTCCAAA
AAGTTTCGGTCTGGCTCATCTTTCTCTCGGCGAGCAGGCTCCAGTGGCAA
CTCCTGCATTACTTACCAGCCATCGGTCTCTGGGGAACACAAGGCACAAG
TGACAACAAAGGCAGAAGTGGAGCCAGGCGTTCACCTTGGTCGTCCTGAT
GTTTTACCTCAGACTCCACCTTTGGAGGAAATCAGTGAGGGCTCGCCTAT
TCCTGACCCCAGTTTCTCACCTCTAGTTGGAGAGACTTTGCAAATGCTAA
CTACAAGTACAACCTTGGAAAAGCTTGAAGTTGCAGAGTCAGAGTTCACC
CATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMASASSGPSSSVGFSSFDP
AVPSCTLSSASGIKRPMASEVPYASGMPIKKIGHRSVDSSGETTYKKTTS
SALKGAIQLGITHTVGSLSTKPERDVLMQDFYVVESIFFPSEGSNLTPAH
HYNDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCSEPLIELCSSGASGSL
FYVSSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCV
QAGGKNIRIVVMNNLLPRSVKMHIKYDLKGSTYKRRASQKEREKPLPTFK
DLDFLQDIPDGLFLDADMYNALCKTLQRDCLVLQSFKIMDYSLLMSIHNI
DHAQREPLSSETQYSVDTRRPAPQKALYSTAMESIQGEARRGGTMETDDH
MGGIPARNSKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVH
RPGFYAERFQRFMCNTVFKKIPLKPSPSKKFRSGSSFSRRAGSSGNSCIT
YQPSVSGEHKAQVTTKAEVEPGVHLGRPDVLPQTPPLEEISEGSPIPDPS
FSPLVGETLQMLTTSTTLEKLEVAESEFTH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32572
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 87954 Daltons