cDNA Clone RUB1 (yeast)

• Plasmid: pGEX6P-1-RUB1 yeast
• Parent Plasmid: pGEX6P1
• Expressed: GST RUB1
• Genbank: 851717
• Tag: GST
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCATGATTGTTAAAGTGAAGACACTGACTGGGAAGGAGATCTCTGT
TGAGCTGAAGGAATCAGATCTCGTATATCACATCAAGGAACTTTTGGAGG
AAAAAGAAGGGATTCCACCATCTCAACAAAGACTTATATTCCAGGGAAAA
CAAATTGATGATAAATTAACAGTAACGGATGCACATCTAGTAGAGGGAAT
GCAACTCCACTTGGTATTAACACTAAGAGGTGGTAACTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMIVKVKTLTGKEISVELKESDLVYHIKELLEEKEGIPPSQQRLIFQGKQIDDKLTVTDAHLVEGMQLHLVLTLRGGN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32468
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast RUB1 – Human Nedd8; ; Estimated MW of expressed protein : 35780 Daltons