cDNA Clone PP4C; PPP4R3A

• Plasmid: pFBDM PP4C, GST PPP4R3A
• Parent Plasmid: pFBDM
• Expressed: untagged PP4C, GST PPP4R3A
• Tag: untagged
• Species: Human

Sequence of Insert

DNA sequence of PP4C insert
GTCGACGAAACCATGGCGGAGATCAGCGACCTGGACCGGCAGATCGAGCA
GCTGCGTCGCTGCGAGCTCATCAAGGAGAGCGAAGTCAAGGCCCTGTGCG
CTAAGGCCAGAGAGATCTTGGTAGAGGAGAGCAACGTGCAGAGGGTGGAC
TCGCCAGTCACAGTGTGCGGCGACATCCATGGACAATTCTATGACCTCAA
AGAGCTGTTCAGAGTAGGTGGCGACGTCCCTGAGACCAACTACCTCTTCA
TGGGGGACTTTGTGGACCGTGGCTTCTATAGCGTCGAAACGTTCCTCCTG
CTGCTGGCACTTAAGGTTCGCTATCCTGATCGCATCACACTGATCCGGGG
CAACCATGAGAGTCGCCAGATCACGCAGGTCTATGGCTTCTACGATGAGT
GCCTGCGCAAGTACGGCTCGGTGACTGTGTGGCGCTACTGCACTGAGATC
TTTGACTACCTCAGCCTGTCAGCCATCATCGATGGCAAGATCTTCTGCGT
GCACGGGGGCCTCTCCCCCTCCATCCAGACCCTGGATCAGATTCGGACAA
TCGACCGAAAGCAAGAGGTGCCTCATGATGGGCCCATGTGTGACCTCCTC
TGGTCTGACCCAGAAGACACCACAGGCTGGGGCGTGAGCCCCCGAGGAGC
CGGCTACCTATTTGGCAGTGACGTGGTGGCCCAGTTCAACGCAGCCAATG
ACATTGACATGATCTGCCGTGCCCACCAACTGGTGATGGAAGGTTACAAG
TGGCACTTCAATGAGACGGTGCTCACTGTGTGGTCGGCACCCAACTACTG
CTACCGCTGTGGGAATGTGGCAGCCATCTTGGAGCTGGACGAGCATCTCC
AGAAAGATTTCATCATCTTTGAGGCTGCTCCCCAAGAGACACGGGGCATC
CCCTCCAAGAAGCCCGTGGCCGACTACTTCCTGTGAGTCGAC

DNA sequence of PPP4R3A insert
GTCGACGAAACCATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCT
TGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAG
AGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTT
GAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGT
TAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACA
ACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAA
GGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAA
AGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGC
TGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGAT
CATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTT
ATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTA
AAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGC
AAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGG
CGACCATCCTCCAAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGG
GATCCCCGGAATTCATGACCGACACCCGGCGGCGGGTGAAGGTGTACACG
CTCAACGAGGACCGGCAGTGGGACGACCGGGGCACCGGGCATGTGTCGTC
TGGCTACGTGGAGCGGCTGAAGGGCATGTCCCTGCTTGTCAGGGCTGAGA
GCGACGGTTCTCTACTTTTAGAGTCGAAAATAAATCCTAACACTGCATAC
CAGAAACAACAGGACACTCTGATTGTGTGGTCTGAAGCAGAAAATTATGA
CTTGGCCCTTAGCTTTCAAGAAAAAGCTGGATGTGATGAAATTTGGGAGA
AAATATGTCAGGTTCAAGGAAAGGACCCTTCCGTGGACATCACTCAGGAC
CTTGTGGATGAATCTGAAGAGGAGCGTTTTGATGATATGTCATCGCCAGG
CTTAGAATTGCCATCTTGTGAATTAAGTCGCCTTGAAGAAATTGCAGAAC
TTGTGGCATCATCTTTACCTTCACCTCTTCGTCGTGAAAAACTTGCACTG
GCACTAGAAAATGAGGGTTATATTAAAAAGCTCCTGGAGCTTTTTCATGT
GTGTGAAGATTTGGAAAATATTGAAGGACTGCACCACTTGTATGAAATTA
TCAAAGGCATCTTTCTCTTGAATCGAACTGCTCTTTTTGAAGTTATGTTC
TCTGAAGAATGTATAATGGACGTCATTGGATGTTTAGAATATGATCCTGC
TTTATCACAACCACGAAAACACAGGGAATTTCTAACAAAAACAGCCAAGT
TTAAAGAAGTGATTCCCATATCAGATCCTGAGCTGAAACAAAAAATTCAT
CAGACATACAGAGTTCAGTATATACAAGATATGGTTCTACCAACTCCTTC
GGTCTTTGAAGAAAACATGTTATCAACACTTCACTCTTTTATCTTTTTCA
ATAAGGTAGAGATTGTTGGCATGTTGCAGGAAGATGAAAAATTTCTGACA
GATTTGTTTGCACAACTAACAGATGAAGCAACAGATGAGGAAAAAAGACA
GGAATTGGTTAACTTTTTAAAAGAATTTTGTGCGTTTTCCCAAACGCTAC
AGCCTCAAAACAGAGATGCTTTTTTCAAGACTTTGTCAAACATGGGCATA
TTACCAGCTTTAGAAGTCATCCTTGGCATGGATGATACACAGGTGCGAAG
TGCTGCTACTGATATATTCTCATACTTGGTTGAATATAATCCATCCATGG
TACGAGAGTTTGTCATGCAGGAGGCACAACAGAATGATGATGTAAGTAAG
AAGTTAACAGAGCAAAAAATAACCAGCAAGGATATTTTGCTCATCAACCT
CATTATAGAACATATGATTTGTGATACAGATCCTGAACTTGGAGGAGCAG
TCCAGCTTATGGGCCTGCTTCGAACTTTAGTTGACCCAGAGAACATGCTA
GCCACTGCCAATAAAACAGAAAAGACTGAATTTCTGGGTTTCTTCTACAA
GCACTGTATGCATGTTCTCACTGCTCCTTTACTAGCAAATACAACAGAAG
ACAAACCTAGTAAAGATGATTTTCAGACTGCCCAACTATTGGCACTTGTA
TTGGAATTGTTAACATTTTGTGTGGAGCACCATACCTACCACATAAAGAA
CTACATTATTAATAAGGATATCCTCCGGAGAGTGCTAGTTCTTATGGCCT
CGAAGCATGCTTTCTTGGCATTATGTGCCCTTCGTTTTAAAAGAAAGATT
ATTGGATTAAAAGATGAGTTTTACAACCGCTACATAATGAAAAGTTTTTT
GTTTGAACCAGTAGTGAAAGCATTTCTCAACAATGGATCCCGCTACAATC
TGATGAACTCTGCCATAATAGAGATGTTTGAATTTATTAGAGTGGAAGAT
ATAAAATCATTAACTGCTCATGTAATTGAAAATTACTGGAAAGCACTGGA
AGATGTAGATTATGTACAGACATTTAAAGGATTAAAACTGAGATTTGAAC
AACAAAGAGAAAGGCAAGATAATCCCAAACTTGACAGTATGCGTTCCATT
TTGAGGAATCACAGATATCGAAGAGATGCCAGAACACTAGAAGATGAAGA
AGAGATGTGGTTTAACACAGATGAAGATGACATGGAAGATGGAGAAGCTG
TAGTGTCTCCATCTGACAAAACTAAAAATGATGATGATATTATGGATCCA
ATAAGTAAATTCATGGAAAGGAAGAAATTAAAAGAAAGTGAGGAAAAGGA
AGTGCTTCTGAAAACAAACCTTTCTGGACGGCAGAGCCCAAGTTTCAAGC
TTTCCCTGTCCAGTGGAACGAAGACTAACCTCACCAGCCAGTCATCTACA
ACAAATCTGCCTGGTTCTCCGGGATCACCTGGATCCCCAGGATCTCCAGG
CTCTCCTGGATCCGTACCTAAAAATACATCTCAGACGGCAGCTATTACTA
CAAAGGGAGGCCTCGTGGGTCTGGTAGATTATCCTGATGATGATGAAGAT
GATGATGAGGATGAAGATAAGGAAGATACGTTACCATTGTCAAAGAAAGC
AAAATTTGATTCATAAGTCGAC

Amino Acid Sequence

Protein sequence of PP4C
MAEISDLDRQIEQLRRCELIKESEVKALCAKAREILVEESNVQRVDSPVT
VCGDIHGQFYDLKELFRVGGDVPETNYLFMGDFVDRGFYSVETFLLLLAL
KVRYPDRITLIRGNHESRQITQVYGFYDECLRKYGSVTVWRYCTEIFDYL
SLSAIIDGKIFCVHGGLSPSIQTLDQIRTIDRKQEVPHDGPMCDLLWSDP
EDTTGWGVSPRGAGYLFGSDVVAQFNAANDIDMICRAHQLVMEGYKWHFN
ETVLTVWSAPNYCYRCGNVAAILELDEHLQKDFIIFEAAPQETRGIPSKK
PVADYFL*

Protein sequence of GST PPP4R3A
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMTDTRRRVKVYTLNED
RQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQ
DTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDE
SEEERFDDMSSPGLELPSCELSRLEEIAELVASSLPSPLRREKLALALEN
EGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEVMFSEEC
IMDVIGCLEYDPALSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYR
VQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFA
QLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPAL
EVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDVSKKLTE
QKITSKDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVDPENMLATAN
KTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELL
TFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLK
DEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSL
TAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNH
RYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKF
MERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLP
GSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGGLVGLVDYPDDDEDDDED
EDKEDTLPLSKKAKFDS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32455
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Untagged PP4C and GST PPP4R3A for baculovirus expression; ; Estimated MW of PP4C : 35078 Daltons; Estimated MW of GTS PPP4R3A : 122563 Daltons