cDNA Clone UBA3 (yeast)

• Synonyms: UBA3, Ubiquitin-Activating Enzyme E1 Homolog
• Plasmid: pGEX6P-1-UBA3 yeast
• Parent Plasmid: pGEX6P1
• Expressed: GST UBA3
• Genbank: 856179
• Tag: GST
• Species: Saccharomyces cerevisiae

Sequence of Insert

ATGGACTGCAAGATATTGGTGTTAGGCGCAGGTGGTCTAGGATGCGAAATCCTGAAGAATTTGACAATGTTAAGCTTTGTTAAACAGGTTCATATTGTAGATATAGACACAATCGAGCTAACCAACCTTAATAGACAATTTCTTTTTTGTGATAAGGACATAGGCAAACCGAAAGCTCAAGTGGCAGCCCAGTATGTAAACACGCGGTTCCCTCAATTGGAAGTTGTGGCGCACGTACAGGACCTGACCACTTTGCCCCCGAGCTTTTACAAAGACTTCCAGTTCATAATCTCGGGGCTGGACGCCATTGAACCTCGTCGCTTCATCAATGAGACTCTCGTGAAGCTTACCTTGGAGTCCAATTATGAAATTTGCATCCCGTTCATAGATGGTGGAACAGAGGGGCTCAAGGGACATGTAAAAACAATCATTCCAGGCATCACTGCATGCTGGGAGTGCTCCATTGATACCTTGCCCTCGCAGCAGGATACTGTGCCAATGTGTACAATCGCCAACAATCCTCGCTGCATTGAGCATGTTGTGGAGTATGTCTCAACTATACAATACCCTGATTTAAATATTGAGTCTACGGCGGATATGGAATTTCTGCTGGAGAAGTGCTGCGAAAGGGCTGCCCAATTTAGCATCTCAACAGAAAAATTATCAACAAGTTTTATCCTGGGTATAATAAAGAGTATCATACCTAGTGTAAGCACTACGAATGCGATGGTTGCAGCGACATGCTGTACGCAGATGGTTAAGATTTATAATGATTTAATCGACCTGGAAAACGGCAATAATTTCACTTTGATTAACTGCTCAGAAGGGTGTTTTATGTACAGTTTCAAGTTCGAGCGGTTACCCGACTGTACTGTTTGTTCAAATTCCAATTCCAACTAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDCKILVLGAGGLGCEILKNLTMLSFVKQVHIVDIDTIELTNLNRQFLFCDKDIGKPKAQVAAQYVNTRFPQLEVVAHVQDLTTLPPSFYKDFQFIISGLDAIEPRRFINETLVKLTLESNYEICIPFIDGGTEGLKGHVKTIIPGITACWECSIDTLPSQQDTVPMCTIANNPRCIEHVVEYVSTIQYPDLNIESTADMEFLLEKCCERAAQFSISTEKLSTSFILGIIKSIIPSVSTTNAMVAATCCTQMVKIYNDLIDLENGNNFTLINCSEGCFMYSFKFERLPDCTVCSNSNSN

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32450
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 60089 Daltons