cDNA Clone Pellino2 (mouse)

• Plasmid: pGEX6P-1-Pellino2 (mouse)
• Parent Plasmid: pGEX6P1
• Expressed: GST Pellino2
• Genbank: EDL20766.1
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGTTTCAGGTGGGCAGGTCAACAGAAAGCCCCATTGACTTCGT
GGTCACAGACACGGTTTCCGGCGGTCAGAACGAAGATGCCCAGATCACAC
AGAGCACCATCTCTAGGTTCGCATGCAGGATCGTGTGTGACAGGAACGAG
CCATACACAGCACGCATATTTGCGGCAGGATTCGATTCTTCCAAAAATAT
CTTTCTTGGAGAGAAAGCAGCAAAATGGAAAAACCCTGATGGACACATGG
ATGGACTCACTACCAACGGTGTCCTGGTGATGCACCCGCAAGGAGGCTTC
ACCGAGGAATCCCAGCCTGGAGTCTGGAGAGAGATCTCTGTCTGTGGGGA
TGTGTACACCTTGCGAGAGACCAGGTCGGCCCAGCAGAGGGGAAAGCTGG
TGGAAAGTGAGACCAACGTCCTGCAAGACGGCTCCCTCATTGACCTGTGT
GGGGCCACTCTCCTCTGGAGAACCGCAGATGGCCTTTTTCACGCTCCTAC
TCAGAAGCACATAGAAGCCCTCCGGCAGGAGATCAATGCAGCCCGACCCC
AGTGCCCCGTGGGCCTTAACACCCTGGCCTTCCCCAGCATCAACCGGAAG
GAAGTGGTGGAAGAGAAGCAGCCCTGGGCATACCTGAGCTGCGGCCATGT
GCACGGCTACCACAGCTGGGGCCATCGGAGCGACGCGGAAGCCAACGAGA
GGGAGTGTCCCATGTGCAGGACTGTGGGCCCCTACGTCCCTCTCTGGCTG
GGCTGTGAGGCAGGATTTTATGTCGATGCGGGACCCCCAACTCACGCTTT
CACCCCCTGCGGGCACGTCTGTTCAGAGAAGTCTGCCAAGTACTGGTCGC
AGATCCCACTGCCCCACGGAACGCACGCGTTTCACGCCGCCTGTCCGTTC
TGCGCCACGCAGCTGGTTGGTGAACAGAACTGCATCAAATTGATTTTCCA
AGGTCCAGTGGACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMFQVGRSTESPIDFVVTDT
VSGGQNEDAQITQSTISRFACRIVCDRNEPYTARIFAAGFDSSKNIFLGE
KAAKWKNPDGHMDGLTTNGVLVMHPQGGFTEESQPGVWREISVCGDVYTL
RETRSAQQRGKLVESETNVLQDGSLIDLCGATLLWRTADGLFHAPTQKHI
EALRQEINAARPQCPVGLNTLAFPSINRKEVVEEKQPWAYLSCGHVHGYH
SWGHRSDAEANERECPMCRTVGPYVPLWLGCEAGFYVDAGPPTHAFTPCG
HVCSEKSAKYWSQIPLPHGTHAFHAACPFCATQLVGEQNCIKLIFQGPVD
*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32332
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; Estimated MW of expressed protein 61956 Daltons