cDNA Clone PIP5K1A

• Plasmid: pFBHTb PIP5K1A
• Parent Plasmid: pFastBac HTb
• Expressed: 6His PIP5K1A
• Genbank: NP_003548.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGTCGGCCTCCTCCGGGCCGTCGTCTTCGGTCGGTTTTTC
ATCCTTTGATCCCGCGGTCCCTTCCTGTACCTTGTCCTCAGCATCTGGAA
TCAAGAGACCCATGGCATCTGAGGTGCCTTATGCCTCTGGCATGCCCATC
AAGAAAATAGGCCATAGAAGTGTTGATTCCTCAGGAGAGACAACATATAA
AAAGACAACCTCATCAGCCTTGAAAGGTGCCATCCAGTTAGGCATTACCC
ACACTGTGGGGAGCCTGAGTACCAAACCAGAGCGTGATGTCCTCATGCAA
GATTTCTACGTGGTTGAGAGTATCTTCTTTCCCAGTGAAGGGAGCAACCT
GACCCCTGCTCATCACTACAATGACTTTCGTTTCAAGACCTATGCACCTG
TTGCCTTCCGCTACTTCCGGGAGCTATTTGGTATCCGGCCCGATGATTAC
TTGTATTCCCTCTGCAGTGAGCCGCTGATTGAACTCTGTAGCTCTGGAGC
TAGTGGTTCCCTATTCTATGTGTCCAGCGACGATGAGTTCATTATTAAGA
CAGTCCAACATAAAGAGGCGGAATTTCTGCAGAAGCTGCTTCCAGGATAC
TACATGAACCTCAACCAGAACCCTCGGACTTTGCTGCCTAAATTCTATGG
ACTGTACTGTGTGCAGGCAGGTGGCAAGAACATTCGGATTGTGGTGATGA
ACAATCTTTTACCAAGATCGGTAAAAATGCATATCAAATATGACCTCAAA
GGCTCAACCTACAAACGGCGGGCTTCCCAGAAAGAGCGAGAGAAGCCTCT
TCCCACATTTAAAGACCTAGACTTCTTACAAGACATCCCTGATGGTCTTT
TTTTGGATGCTGACATGTACAACGCTCTCTGTAAGACCCTGCAGCGTGAC
TGTTTGGTGCTGCAGAGCTTCAAGATAATGGATTACAGCCTCTTGATGTC
AATCCATAATATAGATCATGCACAACGAGAGCCCTTAAGCAGTGAAACAC
AGTACTCAGTTGATACTCGAAGACCGGCCCCCCAAAAGGCTCTGTATTCC
ACAGCCATGGAATCCATCCAGGGAGAGGCTCGACGGGGTGGTACCATGGA
GACTGATGACCATATGGGTGGCATCCCTGCCCGGAATAGTAAAGGGGAAA
GGCTTCTGCTTTATATTGGCATCATTGACATTCTACAGTCTTACAGGTTT
GTTAAGAAGTTGGAGCACTCCTGGAAAGCCCTGGTACATGACGGAGACAC
TGTCTCAGTGCATCGCCCAGGCTTCTACGCTGAACGGTTCCAGCGCTTCA
TGTGCAACACAGTATTTAAGAAGATTCCCTTGAAGCCTTCTCCTTCCAAA
AAGTTTCGGTCTGGCTCATCTTTCTCTCGGCGAGCAGGCTCCAGTGGCAA
CTCCTGCATTACTTACCAGCCATCGGTCTCTGGGGAACACAAGGCACAAG
TGACAACAAAGGCAGAAGTGGAGCCAGGCGTTCACCTTGGTCGTCCTGAT
GTTTTACCTCAGACTCCACCTTTGGAGGAAATCAGTGAGGGCTCGCCTAT
TCCTGACCCCAGTTTCTCACCTCTAGTTGGAGAGACTTTGCAAATGCTAA
CTACAAGTACAACCTTGGAAAAGCTTGAAGTTGCAGAGTCAGAGTTCACC
CATTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMASASSGPSSSVGFSSFDPAVP
SCTLSSASGIKRPMASEVPYASGMPIKKIGHRSVDSSGETTYKKTTSSAL
KGAIQLGITHTVGSLSTKPERDVLMQDFYVVESIFFPSEGSNLTPAHHYN
DFRFKTYAPVAFRYFRELFGIRPDDYLYSLCSEPLIELCSSGASGSLFYV
SSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQAG
GKNIRIVVMNNLLPRSVKMHIKYDLKGSTYKRRASQKEREKPLPTFKDLD
FLQDIPDGLFLDADMYNALCKTLQRDCLVLQSFKIMDYSLLMSIHNIDHA
QREPLSSETQYSVDTRRPAPQKALYSTAMESIQGEARRGGTMETDDHMGG
IPARNSKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHRPG
FYAERFQRFMCNTVFKKIPLKPSPSKKFRSGSSFSRRAGSSGNSCITYQP
SVSGEHKAQVTTKAEVEPGVHLGRPDVLPQTPPLEEISEGSPIPDPSFSP
LVGETLQMLTTSTTLEKLEVAESEFTH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32318
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for baculovirus expression; ; Estimated MW of expressed protein : 64517 Daltons