cDNA Clone PIP5K1B

• Plasmid: pFBHTb PIP5K1B
• Parent Plasmid: pFastBac HTb
• Expressed: 6His PIP5K1B
• Genbank: AAH30587.1
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCGATGTCTTCTGCTGCTGAAAATGGAGAGGCAGCACCTGGAAA
ACAAAATGAAGAAAAAACCTATAAAAAGACACAAGTGAAAAACCAAATCC
TGTCTCGTTTACCCAAGATTCCGTGTACATTCATTCAAGCATGGACCAAC
ACAGAGATGATTACGTTGCTAGCCTGTTATTACTTCAGATCAGTTTCAAC
TGCATCATCTGCTATTAAAGGTGCTATTCAGCTGGGAATAGGATACACAG
TGGGTAATCTCACTTCCAAGCCAGAACGAGATGTTCTTATGCAAGACTTT
TATGTGGTGGAAAGTGTGTTCCTACCCAGCGAAGGGAGCAATCTGACCCC
AGCACATCACTACCCAGACTTTAGATTTAAGACATACGCTCCATTAGCAT
TCCGATATTTCAGAGAACTTTTTGGTATCAAGCCTGATGATTACTTGTAT
TCCATCTGCAGTGAACCTCTAATAGAACTGTCTAACCCTGGAGCCAGTGG
ATCCTTGTTTTTTGTGACCAGTGATGATGAATTTATCATCAAAACAGTTC
AGCACAAAGAAGCTGAGTTTCTTCAGAAGCTACTGCCAGGCTATTACATG
AATTTAAACCAGAATCCAAGGACTCTTTTGCCAAAATTTTACGGACTGTA
TTGTATGCAATCAGGAGGCATTAATATCAGGATTGTGGTGATGAACAACG
TTTTGCCACGCTCCATGAGAATGCACTTTACATATGACTTGAAAGGCTCA
ACGTATAAGCGAAGAGCATCCCGTAAAGAGAGAGAGAAATCCAACCCCAC
ATTTAAGGACTTAGATTTCCTGCAAGACATGCACGAAGGGTTGTATTTTG
ATACGGAAACATACAACGCGCTTATGAAAACACTTCAGAGAGACTGCCGG
GTGCTAGAAAGCTTCAAGATCATGGATTATAGCCTTCTGTTGGGAATTCA
TTTCCTGGACCATTCCCTCAAAGAGAAAGAGGAGGAGACCCCACAAAATG
TGCCTGATGCTAAGCGGACTGGGATGCAGAAGGTTCTCTACTCAACAGCC
ATGGAATCTATCCAGGGTCCAGGGAAATCTGGAGATGGGATAATCACAGA
GAACCCAGACACAATGGGAGGCATTCCAGCTAAAAGCCATAGGGGAGAAA
AACTACTTTTATTTACGGGCATTATTGACATTCTGCAATCATATAGGTTA
ATGAAGAAGTTAGAACATTCCTGGAAAGCTCTTGTTTATGATGGGGACAC
TGTTTCTGTTCATAGACCAAGCTTTTATGCAGACAGATTTCTTAAGTTCA
TGAATTCCAGAGTTTTCAAGAAAATTCAAGCTTTGAAGGCTTCACCGTCT
AAGAAACGGTGCAATTCAATCGCCGCCCTAAAGGCCACTTCACAGGAGAT
TGTGTCCTCAATTAGCCAGGAATGGAAGGATGAGAAGCGGGATTTGCTGA
CTGAAGGACAAAGTTTTAGCAGCCTTGATGAAGAAGCCCTGGGATCCCGA
CACAGGCCAGACCTGGTCCCTAGCACTCCATCACTGTTTGAAGCTGCTTC
CTTGGCAACCACAATTTCATCTTCTTCCTTATACGTCAATGAGCACTATC
CACACGACAGGCCTACACTCTATTCAAACAGGTTCAAGATGGCAACATCA
GAGCATTAAGCGGCCGCG

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSGIQRPTSTSSLVAAAMSSAAEN
GEAAPGKQNEEKTYKKTQVKNQILSRLPKIPCTFIQAWTNTEMITLLACY
YFRSVSTASSAIKGAIQLGIGYTVGNLTSKPERDVLMQDFYVVESVFLPS
EGSNLTPAHHYPDFRFKTYAPLAFRYFRELFGIKPDDYLYSICSEPLIEL
SNPGASGSLFFVTSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLL
PKFYGLYCMQSGGINIRIVVMNNVLPRSMRMHFTYDLKGSTYKRRASRKE
REKSNPTFKDLDFLQDMHEGLYFDTETYNALMKTLQRDCRVLESFKIMDY
SLLLGIHFLDHSLKEKEEETPQNVPDAKRTGMQKVLYSTAMESIQGPGKS
GDGIITENPDTMGGIPAKSHRGEKLLLFTGIIDILQSYRLMKKLEHSWKA
LVYDGDTVSVHRPSFYADRFLKFMNSRVFKKIQALKASPSKKRCNSIAAL
KATSQEIVSSISQEWKDEKRDLLTEGQSFSSLDEEALGSRHRPDLVPSTP
SLFEAASLATTISSSSLYVNEHYPHDRPTLYSNRFKMATSEH

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32293
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for baculovirus expression; ; Estimated MW of expressed protein : 67224 Daltons