cDNA Clone UBC12

• Plasmid: pET156P UBC12
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His UBC12
• Genbank: AAH58924
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCGATGATCAAGCTGTTCTCGCTGAAGCAGCAGAAGAAGGAGGA
GGAGTCGGCGGGCGGCACCAAGGGCAGCAGCAAGAAGGCGTCGGCGGCGC
AGCTGCGGATCCAGAAGGACATAAACGAGCTGAACCTGCCCAAGACGTGT
GATATCAGCTTCTCAGATCCAGACGACCTCCTCAACTTCAAGCTGGTCAT
CTGTCCTGATGAGGGCTTCTACAAGAGTGGGAAGTTTGTGTTCAGTTTTA
AGGTGGGCCAGGGTTACCCGCATGATCCCCCCAAGGTGAAGTGTGAGACA
ATGGTCTATCACCCCAACATTGACCTCGAGGGCAACGTCTGCCTCAACAT
CCTCAGAGAGGACTGGAAGCCAGTCCTTACGATAAACTCCATAATTTATG
GCCTGCAGTATCTCTTCTTGGAGCCCAACCCCGAGGACCCACTGAACAAG
GAGGCCGCAGAGGTCCTGCAGAACAACCGGCGGCTGTTTGAGCAGAACGT
GCAGCGCTCCATGCGGGGTGGCTACATCGGCTCCACCTACTTTGAGCGCT
GCCTGAAATAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSPEFPGVDSKAAAMIKLFSLKQQKKEEE
SAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVIC
PDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNIL
REDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQ
RSMRGGYIGSTYFERCLK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU15804
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 24514 Daltons