cDNA Clone A20 (mouse)

• Plasmid: pFBDual6P-1 A20 (mouse)
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST-A20 (mouse)
• Genbank: AAH60221
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGGCTGAACAACTTCTTCCTCAGGCTTTGTATTTGAGCAATAT
GCGGAAAGCTGTGAAGATACGAGAGAGAACCCCAGAAGACATTTTCAAAC
CTACCAATGGGATCATCTATCACTTTAAAACCATGCACCGATACACGCTG
GAGATGTTCAGAACATGCCAGTTTTGCCCACAGTTCCGAGAGATCATCCA
CAAAGCACTTATTGACAGAAGTGTCCAGGCTTCCCTGGAAAGCCAGAAGA
AGCTCAACTGGTGTCGTGAAGTCAGGAAGCTCGTGGCTCTGAAAACCAAT
GGTGATGGAAACTGCCTCATGCATGCAGCTTGTCAGTACATGTGGGGTGT
TCAGGATACTGACCTGGTCCTGAGGAAGGCCCTCTGCAGCACCCTTAAGG
AGACAGACACTCGGAACTTTAAATTCCGCTGGCAGCTGGAATCTCTGAAA
TCTCAGGAATTTGTGGAAACAGGACTTTGCTACGACACTCGGAACTGGAA
TGACGAATGGGACAACTTGGTCAAAATGGCATCAGCAGACACACCTGCAG
CCCGAAGTGGACTTCAGTACAATTCCCTGGAAGAAATCCACATATTTGTC
CTCAGCAACATCCTCAGAAGACCCATCATTGTCATTTCAGACAAAATGCT
AAGAAGTTTGGAATCTGGTTCCAATTTTGCTCCTTTGAAAGTGGGTGGGA
TTTATCTGCCTCTTCACTGGCCTGCCCAGGAGTGTTACAGATATCCCATC
GTCCTAGGCTATGACAGCCAGCACTTTGTACCCCTGGTGACCCTGAAGGA
CAGTGGACCTGAACTTCGCGCTGTTCCACTTGTTAACAGAGACCGGGGTA
GGTTTGAAGACTTAAAAGTTCACTTCTTGACAGATCCTGAGAATGAGATG
AAGGAAAAGCTTCTAAAGGAGTACTTGATAGTGATGGAGATCCCTGTGCA
AGGCTGGGACCACGGCACGACTCACCTGATCAACGCTGCAAAATTGGATG
AAGCTAACTTACCCAAAGAAATAAATTTGGTAGACGATTACTTTGAGCTT
GTTCAGCACGAATACAAGAAATGGCAGGAGAACAGCGATCAGGCCAGGAG
AGCGGCACATGCGCAGAACCCCTTGGAGCCTTCCACACCCCAGCTATCAC
TCATGGATATAAAATGTGAGACACCCAACTGTCCTTTCTTCATGTCCGTG
AACACTCAGCCTTTATTCCACGAATGCTCAGAGAGGCGCCAAAAGAATCA
GAGCAAGCTCCCAAAGCTGAACTCGAAGCTAGGCCCTGAAGGACTCCCAG
GCGTGGGACTTGGCTCCTCAAACTGGAGCCCCGAGGAAACCGCTGGAGGA
CCTCATTCAGCCCCACCCACAGCACCCAGCCTTTTTCTCTTCAGTGAGAC
CACTGCAATGAAGTGCAGGAGTCCTGGGTGCCCTTTTACTTTGAATGTGC
AGCATAATGGATTCTGTGAGCGTTGCCACGCCCGGCAGATTAATGCCAGC
CACACCGCAGACCCTGGAAAGTGCCAAGCCTGCCTTCAGGATGTCACTCG
GACCTTTAATGGCATCTGCAGTACCTGTTTCAAAAGGACTACAGCAGAGC
CCAGCTCCAGCCTCACTTCCAGTATCCCTGCCTCCTGTCACCAACGCTCC
AAGTCTGACCCCTCACAACTCATCCAAAGTCTCACTCCACACTCTTGCCA
CCGGACTGGAAATGTCTCTCCTTCTGGCTGCCTCTCCCAGGCTGCACGGA
CTCCAGGAGACAGAGCAGGGACAAGCAAGTGCAGGAAAGCTGGCTGCATG
TATTTTGGGACTCCAGAAAACAAGGGCTTTTGCACTCTATGTTTCATCGA
ATACAGAGAAAATAAGCAGTCTGTTACTGCCTCTGAGAAAGCTGGTTCCC
CGGCCCCCAGGTTCCAGAACAATGTCCCGTGCCTGGGCAGGGAGTGCGGC
ACACTCGGAAGCACCATGTTTGAAGGGTACTGTCAGAAGTGTTTCATCGA
AGCTCAGAACCAGAGATTCCATGAAGCAAGAAGAACGGAAGAACAGCTGA
GATCAAGCCAGCATAGAGACATGCCTCGAACTACACAGGTAGCCTCAAGG
CTGAAATGTGCCCGGGCCTCCTGCAAGAACATTCTGGCCTGTCGCAGTGA
GGAACTCTGTATGGAGTGCCAGCACCTAAGCCAACGAGTAGGTTCTGTGG
CCCACCGGGGTGAGCCCACGCCTGAAGAGCCCCCTAAACAGCGCTGCCGG
GCCCCTGCTTGTGATCACTTTGGCAATGCCAAGTGTAATGGTTACTGCAA
TGAGTGCTACCAGTTCAAGCAGATGTATGGCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEQLLPQALYLSNMRKAV
KIRERTPEDIFKPTNGIIYHFKTMHRYTLEMFRTCQFCPQFREIIHKALI
DRSVQASLESQKKLNWCREVRKLVALKTNGDGNCLMHAACQYMWGVQDTD
LVLRKALCSTLKETDTRNFKFRWQLESLKSQEFVETGLCYDTRNWNDEWD
NLVKMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVISDKMLRSLE
SGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTLKDSGPE
LRAVPLVNRDRGRFEDLKVHFLTDPENEMKEKLLKEYLIVMEIPVQGWDH
GTTHLINAAKLDEANLPKEINLVDDYFELVQHEYKKWQENSDQARRAAHA
QNPLEPSTPQLSLMDIKCETPNCPFFMSVNTQPLFHECSERRQKNQSKLP
KLNSKLGPEGLPGVGLGSSNWSPEETAGGPHSAPPTAPSLFLFSETTAMK
CRSPGCPFTLNVQHNGFCERCHARQINASHTADPGKCQACLQDVTRTFNG
ICSTCFKRTTAEPSSSLTSSIPASCHQRSKSDPSQLIQSLTPHSCHRTGN
VSPSGCLSQAARTPGDRAGTSKCRKAGCMYFGTPENKGFCTLCFIEYREN
KQSVTASEKAGSPAPRFQNNVPCLGRECGTLGSTMFEGYCQKCFIEAQNQ
RFHEARRTEEQLRSSQHRDMPRTTQVASRLKCARASCKNILACRSEELCM
ECQHLSQRVGSVAHRGEPTPEEPPKQRCRAPACDHFGNAKCNGYCNECYQ
FKQMYG*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU15701
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for baculovirus expression.; ; Estimated MW of expressed protein : 114518 Daltons