cDNA Clone USP17

• Plasmid: pFBHTb USP17
• Parent Plasmid: pFastBac HTb
• Expressed: 6His USP17
• Genbank: NP_001099132
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGAGGACGACTCACTCTACTTGGGAGGTGAGTGGCAGTTCAA
CCACTTTCCAAAACTCACATCTTCTCGGCCCGATGCAGCTTTTGCTGAAA
TCCAGCGGACTTCTCTCCCTGAGAAGTCACCACTCTCATGTGAGACCCGT
GTCGACCTCTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAG
GGAGAAGCTTCCTCTGAGTAGCAGGAGACCTGCTGCGGTGGGGGCTGGGC
TCCAGAATATGGGAAATACCTGCTACGTGAACGCTTCCTTGGAGTGGCTG
ACATACACACCGCCCCTTGCCAACTACATGCTGTCCCGGGAGCACTCTCA
AACGTGTCATCGTCACAAGGGCTGCATGCTCTGTACTATGCAAGCTCACA
TCACACGGGCCCTCCACAATCCTGGCCACGTCATCCAGCCCTCACAGGCA
TTGGCTGCTGGCTTCCATAGAGGCAAGCAGGAAGATGCTCATGAATTTCT
CATGTTCACTGTGGATGCCATGAAAAAGGCATGCCTTCCCGGGCACAAGC
AGGTAGATCATCACTCTAAGGACACCACCCTCATCCACCAAATATTTGGA
GGCTACTGGAGATCTCAAATCAAGTGTCTCCACTGCCACGGCATTTCAGA
CACTTTTGACCCTTACCTGGACATCGCCCTGGATATCCAGGCAGCTCAGA
GTGTCCAGCAAGCTTTGGAACAGTTGGTGAAGCCCGAAGAACTCAATGGA
GAGAATGCCTATCATTGTGGTGTTTGTCTCCAGAGGGCGCCGGCCTCCAA
GACGTTAACTTTACACACCTCTGCCAAGGTCCTCATCCTTGTATTGAAGA
GATTCTCCGATGTCACAGGCAACAAGATTGCCAAGAATGTGCAATATCCT
GAGTGCCTTGACATGCAGCCATACATGTCTCAGCAGAACACAGGACCTCT
CGTCTATGTCCTCTATGCTGTGCTGGTCCACGCTGGGTGGAGTTGTCACA
ACGGACATTACTTCTCTTATGTCAAAGCTCAAGAAGGCCAGTGGTATAAA
ATGGATGATGCCGAGGTCACCGCCTCTAGCATCACTTCTGTCCTGAGTCA
ACAGGCCTACGTCCTCTTTTACATCCAGAAGAGTGAATGGGAAAGACACA
GTGAGAGTGTGTCAAGAGGCAGGGAACCAAGAGCCCTTGGCGCAGAAGAC
ACAGACAGGCGAGCAACGCAAGGAGAGCTCAAGAGAGACCACCCCTGCCT
CCAGGCCCCCGAGTTGGACGAGCACTTGGTGGAAAGAGCCACTCAGGAAA
GCACCTTAGACCACTGGAAATTCCTTCAAGAGCAAAACAAAACGAAGCCT
GAGTTCAACGTCAGAAAAGTCGAAGGTACCCTGCCTCCCGACGTACTTGT
GATTCATCAATCAAAATACAAGTGTGGGATGAAGAACCATCATCCTGAAC
AGCAAAGCTCCCTGCTAAACCTCTCTTCGACGACACCGACACATCAGGAG
TCCATGAACACTGGCACACTCGCTTCCCTGCGAGGGAGGGCCAGGAGATC
CAAAGGGAAGAACAAACACAGCAAGAGGGCTCTGCTTGTGTGCCAGTGAG
CGGCCGC

GTGGCAGAAATTCTCAGGAGCAGCATGCCTTGAAGCACTATCTGACGCCA
AGATCTGAACCTCACTGTCTGGTTCTTAGTTTGGACAACTGGAGTGTATG
GTGTTACGTATGTGATAATGAGGTCCAGTATTGTAGTTCAAACCAGTTGG
GTCAAGTGGTTGATTATGTCAGAAAACATGCCAGCATTACAACTCCAAAG
CCAGAGAAAGATAATGGAAATATTGAACTTGAAAATAAAAAATTAGAAAA
AGAGAGTAAGAATGAACAAGAGAGAGAAAAGAAGGAAAACATGGCTAAAG
AGAATCCTCCCATGAATTCTCCTTGCCAAATAACCGTGAAAGGACTCAGT
AATTTGGGAAACACATGTTTCTTCAATGCAGTTATGCAGAACTTGTCACA
AACACCAGTGCTTAGAGAACTACTAAAAGAAGTGAAAATGTCTGGAACAA
TTGTAAAAATTGAACCACCTGATTTGGCATTAACAGAACCATTAGAAATA
AACCTTGAGCCTCCAGGCCCTCTTACTTTAGCCATGAGCCAGTTTCTTAA
TGAGATGCAAGAGACCAAAAAGGGGGTTGTGACACCGAAAGAACTCTTTT
CTCAGGTCTGTAAAAAAGCAGTGCGGTTTAAAGGCTATCAGCAGCAAGAC
AGCCAGGAGCTGCTTCGCTACTTATTGGATGGGATGAGAGCAGAAGAACA
CCAAAGAGTGAGTAAAGGAATACTTAAAGCATTTGGTAATTCTACTGAAA
AGTTGGATGAAGAACTAAAAAATAAAGTTAAAGATTATGAGAAGAAAAAA
TCAATGCCAAGTTTTGTTGACCGCATCTTTGGTGGTGAACTAACTAGTAT
GATCATGTGTGATCAATGCAGAACTGTCTCCTTGGTTCATGAATCTTTCC
TTGATTTGTCCCTCCCAGTTTTAGATGATCAGAGTGGTAAGAAAAGTGTA
AATGATAAAAATCTGAAAAAGACAGTGGAGGATGAAGATCAAGATAGTGA
GGAAGAAAAAGATAACGACAGTTACATAAAAGAGAGAAGTGATATTCCTT
CTGGAACAAGTAAGCACTTACAGAAAAAAGCAAAGAAACAAGCCAAAAAG
CAAGCCAAGAACCAACGAAGACAACAAAAAATTCAAGGAAAAGTTCTTCA
TTTAAATGATATTTGTACTATTGACCATCCTGAAGACAGTGATAATGAAG
CTGAAATGTCACTTCAAGGAGAAGTAAATATTAAATCCAACCATATTTCA
CAAGAGGGTGTTATGCATAAAGAATATTGTGTCAACCAGAAAGATTTGAA
TGGCCAAGCAAAAATGATCGAAAGTGTAACTGACAATCAAAAATCCACAG
AGGAAGTAGATATGAAAAATATCAACATGGATAATGATCTGGAGGTTTTA
ACATCTTCTCCCACTAGGAATTTAAATGGTGCCTACCTAACGGAAGGGAG
CAATGGAGAAGTGGACATTTCCAATGGTTTCAAAAACCTAAATTTGAATG
CTGCTCTTCATCCTGATGAAATAAATATAGAGATTCTGAATGATAGTCAT
ACTCCTGGAACAAAGGTGTATGAGGTTGTAAATGAAGATCCAGAAACTGC
TTTCTGTACTCTTGCAAACAGGGAAGTTTTCAATACTGATGAGTGTTCAA
TCCAACATTGTTTATATCAGTTCACCCGTAATGAGAAACTTCGAGATGCG
AATAAACTGCTTTGTGAAGTATGCACACGGAGACAGTGTAATGGACCAAA
GGCAAATATAAAAGGTGAAAGGAAGCATGTTTACACCAATGCCAAAAAGC
AGATGCTAATTTCTCTTGCTCCTCCTGTTCTTACTCTTCATTTAAAGAGA
TTTCAGCAGGCTGGTTTTAACCTACGCAAAGTTAACAAACACATAAAGTT
TCCGGAAATCTTAGATTTGGCTCCTTTTTGCACCCTTAAATGTAAGAATG
TTGCAGAAGAAAATACAAGGGTACTCTATTCCTTATATGGAGTTGTTGAA
CACAGTGGTACTATGAGGTCGGGGCATTACACTGCCTATGCCAAGGCAAG
AACCGCAAATAGTCATCTCTCTAATCTTGTTCTTCACGGTGATATTCCAC
AAGATTTTGAAATGGAATCAAAAGGGCAGTGGTTTCACATCAGCGACACA
CATGTGCAAGCTGTGCCTACAACTAAAGTACTAAACTCACAAGCGTACCT
CCTATTTTATGAGAGAATACTGTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMEDDSLYLGGEWQFNHFPKLTS
SRPDAAFAEIQRTSLPEKSPLSCETRVDLCDDLAPVARQLAPREKLPLSS
RRPAAVGAGLQNMGNTCYVNASLEWLTYTPPLANYMLSREHSQTCHRHKG
CMLCTMQAHITRALHNPGHVIQPSQALAAGFHRGKQEDAHEFLMFTVDAM
KKACLPGHKQVDHHSKDTTLIHQIFGGYWRSQIKCLHCHGISDTFDPYLD
IALDIQAAQSVQQALEQLVKPEELNGENAYHCGVCLQRAPASKTLTLHTS
AKVLILVLKRFSDVTGNKIAKNVQYPECLDMQPYMSQQNTGPLVYVLYAV
LVHAGWSCHNGHYFSYVKAQEGQWYKMDDAEVTASSITSVLSQQAYVLFY
IQKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCLQAPELDE
HLVERATQESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDVLVIHQSKYK
CGMKNHHPEQQSSLLNLSSTTPTHQESMNTGTLASLRGRARRSKGKNKHS
KRALLVCQ*

MSGTIVKIEPPDLALTEPLEINLEPPGPLTLAMSQFLNEMQETKKGVVTP
KELFSQVCKKAVRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFG
NSTEKLDEELKNKVKDYEKKKSMPSFVDRIFGGELTSMIMCDQCRTVSLV
HESFLDLSLPVLDDQSGKKSVNDKNLKKTVEDEDQDSEEEKDNDSYIKER
SDIPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQGKVLHLNDICTIDHPED
SDNEAEMSLQGEVNIKSNHISQEGVMHKEYCVNQKDLNGQAKMIESVTDN
QKSTEEVDMKNINMDNDLEVLTSSPTRNLNGAYLTEGSNGEVDISNGFKN
LNLNAALHPDEINIEILNDSHTPGTKVYEVVNEDPETAFCTLANREVFNT
DECSIQHCLYQFTRNEKLRDANKLLCEVCTRRQCNGPKANIKGERKHVYT
NAKKQMLISLAPPVLTLHLKRFQQAGFNLRKVNKHIKFPEILDLAPFCTL
KCKNVAEENTRVLYSLYGVVEHSGTMRSGHYTAYAKARTANSHLSNLVLH
GDIPQDFEMESKGQWFHISDTHVQAVPTTKVLNSQAYLLFYERIL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU15658
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for baculovirus expression.; ; Estimated MW of expressed protein : 63119 Daltons