cDNA Clone USP1

• Plasmid: pGEX6P-1-USP1
• Parent Plasmid: pGEX6P1
• Expressed: GST USP1
• Genbank: NP_003359.3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCCTGGTGTCATACCTAGTGAAAGTAATGGACTTTCAAGAGG
TAGCCCTTCAAAGAAAAACAGACTTTCCTTAAAGTTTTTTCAGAAAAAGG
AAACTAAGAGAGCTTTGGATTTCACAGATTCTCAAGAAAATGAAGAAAAA
GCTTCTGAATATAGAGCATCTGAAATTGATCAAGTTGTTCCTGCAGCACA
GTCTTCACCTATAAACTGTGAGAAGAGAGAAAACTTGTTACCATTTGTGG
GACTGAATAATCTCGGCAATACTTGCTATCTTAATAGTATACTTCAGGTA
TTATATTTTTGTCCCGGTTTTAAATCTGGAGTAAAGCACTTATTTAATAT
TATTTCAAGGAAGAAAGAAGCTCTAAAGGATGAAGCCAATCAAAAAGACA
AGGGAAATTGCAAAGAAGATTCTTTGGCAAGTTATGAATTGATATGCAGT
TTACAGTCCTTAATCATTTCGGTTGAACAGCTCCAGGCTAGTTTTCTCTT
AAATCCAGAGAAATATACTGATGAACTTGCCACTCAGCCAAGGCGACTGC
TTAACACACTGAGGGAACTCAACCCTATGTATGAAGGATATCTACAGCAT
GATGCACAGGAAGTATTACAATGTATTTTGGGAAACATTCAAGAAACATG
CCAACTCCTAAAAAAAGAAGAAGTAAAAAATGTGGCAGAATTACCTACTA
AGGTAGAAGAAATACCTCATCCGAAAGAGGAAATGAATGGTATTAACAGC
ATAGAGATGGACAGTATGAGGCATTCTGAAGACTTTAAAGAGAAACTCCC
AAAAGGAAATGGGAAAAGAAAAAGTGACACTGAATTTGGTAACATGAAGA
AAAAAGTTAAATTATCCAAGGAACACCAGTCATTGGAAGAGAACCAGAGA
CAAACTAGATCAAAAAGAAAAGCTACAAGTGATACATTAGAGAGTCCTCC
TAAAATAATTCCCAAGTATATTTCTGAAAATGAGAGTCCAAGACCCTCAC
AAAAGAAATCAAGAGTTAAAATAAATTGGTTAAAGTCTGCAACTAAGCAA
CCCAGCATTCTTTCTAAATTTTGTAGTCTGGGAAAAATAACAACAAACCA
AGGAGTCAAAGGACAATCTAAAGAAAATGAATGTGATCCTGAAGAGGACT
TGGGGAAGTGTGAAAGTGATAACACAACTAATGGTTGTGGACTTGAATCT
CCAGGAAATACTGTTACACCTGTAAATGTTAATGAAGTTAAACCCATAAA
CAAAGGTGAAGAACAAATTGGTTTTGAGCTAGTGGAGAAATTATTTCAAG
GTCAGCTGGTATTAAGGACGCGTTGCTTGGAATGTGAAAGTTTAACAGAA
AGAAGAGAAGATTTTCAAGACATCAGTGTGCCAGTACAAGAAGATGAGCT
TTCCAAAGTAGAGGAGAGTTCTGAAATTTCTCCAGAGCCAAAAACAGAAA
TGAAGACCCTGAGATGGGCAATTTCACAATTTGCTTCAGTAGAAAGGATT
GTAGGAGAAGATAAATATTTCTGTGAAAACTGCCATCATTATACTGAAGC
TGAACGAAGTCTTTTGTTTGACAAAATGCCTGAAGTTATAACTATTCATT
TGAAGTGCTTTGCTGCTAGTGGTTTGGAGTTTGATTGTTATGGTGGTGGA
CTTTCCAAGATCAACACTCCTTTATTGACACCTCTTAAATTGTCACTAGA
AGAATGGAGCACAAAGCCAACTAACGACAGCTATGGATTATTTGCGGTTG
TGATGCATAGTGGCATTACAATTAGTAGTGGGCATTACACTGCTTCTGTT
AAAGTCACTGACCTTAACAGTTTAGAACTAGATAAAGGAAATTTTGTGGT
TGACCAAATGTGTGAAATAGGTAAGCCAGAACCATTGAATGAGGAGGAAG
CAAGGGGTGTGGTTGAGAATTATAATGATGAAGAAGTGTCAATTAGAGTT
GGTGGAAATACACAGCCAAGTAAAGTTTTGAACAAAAAAAATGTAGAAGC
TATTGGACTTCTTGGAGGACAAAAGAGCAAAGCAGATTATGAGCTATACA
ACAAAGCCTCTAATCCTGATAAGGTTGCTAGTACAGCGTTTGCTGAAAAT
AGAAATTCTGAGACTAGTGATACTACTGGGACCCATGAATCTGATAGAAA
CAAGGAATCCAGTGACCAAACAGGCATTAATATTAGTGGATTTGAGAACA
AAATTTCATACGTAGTGCAAAGCTTAAAGGAGTATGAGGGGAAGTGGTTG
CTTTTTGATGATTCTGAAGTCAAAGTTACTGAAGAGAAGGACTTTCTGAA
TTCTCTTTCCCCTTCTACATCTCCTACTTCTACTCCTTACTTGCTATTTT
ATAAGAAATTATAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPGVIPSESNGLSRGSPSK
KNRLSLKFFQKKETKRALDFTDSQENEEKASEYRASEIDQVVPAAQSSPI
NCEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKSGVKHLFNIISRK
KEALKDEANQKDKGNCKEDSLASYELICSLQSLIISVEQLQASFLLNPEK
YTDELATQPRRLLNTLRELNPMYEGYLQHDAQEVLQCILGNIQETCQLLK
KEEVKNVAELPTKVEEIPHPKEEMNGINSIEMDSMRHSEDFKEKLPKGNG
KRKSDTEFGNMKKKVKLSKEHQSLEENQRQTRSKRKATSDTLESPPKIIP
KYISENESPRPSQKKSRVKINWLKSATKQPSILSKFCSLGKITTNQGVKG
QSKENECDPEEDLGKCESDNTTNGCGLESPGNTVTPVNVNEVKPINKGEE
QIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVE
ESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSL
LFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWST
KPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDKGNFVVDQMC
EIGKPEPLNEEEARGVVENYNDEEVSIRVGGNTQPSKVLNKKNVEAIGLL
GGQKSKADYELYNKASNPDKVASTAFAENRNSETSDTTGTHESDRNKESS
DQTGINISGFENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSP
STSPTSTPYLLFYKKL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU15631
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 115029 Daltons