cDNA Clone PP4R3a

• Plasmid: pCMV-FLAG PP4R3a
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG PP4R3a
• Genbank: AAH72409
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCATGACCGACACCCGGCGGCGGGTGAAGGTGTACACGCTCAACGA
GGACCGGCAGTGGGACGACCGGGGCACCGGGCATGTGTCGTCTGGCTACG
TGGAGCGGCTGAAGGGCATGTCCCTGCTTGTCAGGGCTGAGAGCGACGGT
TCTCTACTTTTAGAGTCGAAAATAAATCCTAACACTGCATACCAGAAACA
ACAGGACACTCTGATTGTGTGGTCTGAAGCAGAAAATTATGACTTGGCCC
TTAGCTTTCAAGAAAAAGCTGGATGTGATGAAATTTGGGAGAAAATATGT
CAGGTTCAAGGAAAGGACCCTTCCGTGGACATCACTCAGGACCTTGTGGA
TGAATCTGAAGAGGAGCGTTTTGATGATATGTCATCGCCAGGCTTAGAAT
TGCCATCTTGTGAATTAAGTCGCCTTGAAGAAATTGCAGAACTTGTGGCA
TCATCTTTACCTTCACCTCTTCGTCGTGAAAAACTTGCACTGGCACTAGA
AAATGAGGGTTATATTAAAAAGCTCCTGGAGCTTTTTCATGTGTGTGAAG
ATTTGGAAAATATTGAAGGACTGCACCACTTGTATGAAATTATCAAAGGC
ATCTTTCTCTTGAATCGAACTGCTCTTTTTGAAGTTATGTTCTCTGAAGA
ATGTATAATGGACGTCATTGGATGTTTAGAATATGATCCTGCTTTATCAC
AACCACGAAAACACAGGGAATTTCTAACAAAAACAGCCAAGTTTAAAGAA
GTGATTCCCATATCAGATCCTGAGCTGAAACAAAAAATTCATCAGACATA
CAGAGTTCAGTATATACAAGATATGGTTCTACCAACTCCTTCGGTCTTTG
AAGAAAACATGTTATCAACACTTCACTCTTTTATCTTTTTCAATAAGGTA
GAGATTGTTGGCATGTTGCAGGAAGATGAAAAATTTCTGACAGATTTGTT
TGCACAACTAACAGATGAAGCAACAGATGAGGAAAAAAGACAGGAATTGG
TTAACTTTTTAAAAGAATTTTGTGCGTTTTCCCAAACGCTACAGCCTCAA
AACAGAGATGCTTTTTTCAAGACTTTGTCAAACATGGGCATATTACCAGC
TTTAGAAGTCATCCTTGGCATGGATGATACACAGGTGCGAAGTGCTGCTA
CTGATATATTCTCATACTTGGTTGAATATAATCCATCCATGGTACGAGAG
TTTGTCATGCAGGAGGCACAACAGAATGATGATGTAAGTAAGAAGTTAAC
AGAGCAAAAAATAACCAGCAAGGATATTTTGCTCATCAACCTCATTATAG
AACATATGATTTGTGATACAGATCCTGAACTTGGAGGAGCAGTCCAGCTT
ATGGGCCTGCTTCGAACTTTAGTTGACCCAGAGAACATGCTAGCCACTGC
CAATAAAACAGAAAAGACTGAATTTCTGGGTTTCTTCTACAAGCACTGTA
TGCATGTTCTCACTGCTCCTTTACTAGCAAATACAACAGAAGACAAACCT
AGTAAAGATGATTTTCAGACTGCCCAACTATTGGCACTTGTATTGGAATT
GTTAACATTTTGTGTGGAGCACCATACCTACCACATAAAGAACTACATTA
TTAATAAGGATATCCTCCGGAGAGTGCTAGTTCTTATGGCCTCGAAGCAT
GCTTTCTTGGCATTATGTGCCCTTCGTTTTAAAAGAAAGATTATTGGATT
AAAAGATGAGTTTTACAACCGCTACATAATGAAAAGTTTTTTGTTTGAAC
CAGTAGTGAAAGCATTTCTCAACAATGGATCCCGCTACAATCTGATGAAC
TCTGCCATAATAGAGATGTTTGAATTTATTAGAGTGGAAGATATAAAATC
ATTAACTGCTCATGTAATTGAAAATTACTGGAAAGCACTGGAAGATGTAG
ATTATGTACAGACATTTAAAGGATTAAAACTGAGATTTGAACAACAAAGA
GAAAGGCAAGATAATCCCAAACTTGACAGTATGCGTTCCATTTTGAGGAA
TCACAGATATCGAAGAGATGCCAGAACACTAGAAGATGAAGAAGAGATGT
GGTTTAACACAGATGAAGATGACATGGAAGATGGAGAAGCTGTAGTGTCT
CCATCTGACAAAACTAAAAATGATGATGATATTATGGATCCAATAAGTAA
ATTCATGGAAAGGAAGAAATTAAAAGAAAGTGAGGAAAAGGAAGTGCTTC
TGAAAACAAACCTTTCTGGACGGCAGAGCCCAAGTTTCAAGCTTTCCCTG
TCCAGTGGAACGAAGACTAACCTCACCAGCCAGTCATCTACAACAAATCT
GCCTGGTTCTCCGGGATCACCTGGATCCCCAGGATCTCCAGGCTCTCCTG
GATCCGTACCTAAAAATACATCTCAGACGGCAGCTATTACTACAAAGGGA
GGCCTCGTGGGTCTGGTAGATTATCCTGATGATGATGAAGATGATGATGA
GGATGAAGATAAGGAAGATACGTTACCATTGTCAAAGAAAGCAAAATTTG
ATTCATAAGTCGAC

Amino Acid Sequence

MDYKDDDDKGSPEFMTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLK
GMSLLVRAESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQE
KAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCE
LSRLEEIAELVASSLPSPLRREKLALALENEGYIKKLLELFHVCEDLENI
EGLHHLYEIIKGIFLLNRTALFEVMFSEECIMDVIGCLEYDPALSQPRKH
REFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLPTPSVFEENML
STLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLK
EFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFS
YLVEYNPSMVREFVMQEAQQNDDVSKKLTEQKITSKDILLINLIIEHMIC
DTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLT
APLLANTTEDKPSKDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDI
LRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKA
FLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQT
FKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTD
EDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNL
SGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPK
NTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15604
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression.; ; Estimated MW of expressed protein : 97009 Daltons